MIM2_YEAST
ID MIM2_YEAST Reviewed; 87 AA.
AC Q3E798; D6VYA0;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Mitochondrial import protein 2 {ECO:0000303|PubMed:22467864};
GN Name=MIM2 {ECO:0000303|PubMed:22467864}; OrderedLocusNames=YLR099W-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=11152879; DOI=10.1016/s0014-5793(00)02275-4;
RA Blandin G., Durrens P., Tekaia F., Aigle M., Bolotin-Fukuhara M., Bon E.,
RA Casaregola S., de Montigny J., Gaillardin C., Lepingle A., Llorente B.,
RA Malpertuy A., Neuveglise C., Ozier-Kalogeropoulos O., Perrin A., Potier S.,
RA Souciet J.-L., Talla E., Toffano-Nioche C., Wesolowski-Louvel M., Marck C.,
RA Dujon B.;
RT "Genomic exploration of the hemiascomycetous yeasts: 4. The genome of
RT Saccharomyces cerevisiae revisited.";
RL FEBS Lett. 487:31-36(2000).
RN [4]
RP SUBCELLULAR LOCATION, FUNCTION, SUBUNIT, INTERACTION WITH MIM1, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=22467864; DOI=10.1242/jcs.103804;
RA Dimmer K.S., Papic D., Schumann B., Sperl D., Krumpe K., Walther D.M.,
RA Rapaport D.;
RT "A crucial role for Mim2 in the biogenesis of mitochondrial outer membrane
RT proteins.";
RL J. Cell Sci. 125:3464-3473(2012).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA He C., Jia C., Zhang Y., Xu P.;
RT "Enrichment-based proteogenomics identifies microproteins, missing
RT proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL J. Proteome Res. 17:2335-2344(2018).
CC -!- FUNCTION: Component of the MIM complex required for outer membrane
CC protein import (PubMed:22467864). Involved in import of the subset of
CC proteins with multiple alpha-helical transmembrane segments, including
CC UGO1, TOM20 and FZO1 (PubMed:22467864). {ECO:0000269|PubMed:22467864}.
CC -!- SUBUNIT: Component of the MIM complex containing at least MIM1 and MIM2
CC (PubMed:22467864). Interacts with MIM1; interaction is direct
CC (PubMed:22467864). {ECO:0000269|PubMed:22467864}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:22467864}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Causes severe growth phenotype and leads to
CC reduced biogenesis of mitochondrial proteins and abnormal mitochondrial
CC morphology (PubMed:22467864). Compromises import of multispan
CC mitochondrial outer membrane (MOM) proteins (PubMed:22467864).
CC {ECO:0000269|PubMed:22467864}.
CC -!- SIMILARITY: Belongs to the MIM2 family. {ECO:0000305}.
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DR EMBL; U53876; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006945; DAA09416.1; -; Genomic_DNA.
DR RefSeq; NP_076901.1; NM_001184484.1.
DR AlphaFoldDB; Q3E798; -.
DR SMR; Q3E798; -.
DR BioGRID; 31373; 6.
DR STRING; 4932.YLR099W-A; -.
DR TCDB; 1.B.33.3.1; the outer membrane protein insertion porin (bam complex) (ompip) family.
DR MaxQB; Q3E798; -.
DR PaxDb; Q3E798; -.
DR PRIDE; Q3E798; -.
DR EnsemblFungi; YLR099W-A_mRNA; YLR099W-A; YLR099W-A.
DR GeneID; 850789; -.
DR KEGG; sce:YLR099W-A; -.
DR SGD; S000007618; MIM2.
DR VEuPathDB; FungiDB:YLR099W-A; -.
DR eggNOG; ENOG502SBHE; Eukaryota.
DR HOGENOM; CLU_186257_0_0_1; -.
DR InParanoid; Q3E798; -.
DR BioCyc; YEAST:G3O-32570-MON; -.
DR PRO; PR:Q3E798; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q3E798; protein.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0070096; P:mitochondrial outer membrane translocase complex assembly; IGI:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IMP:SGD.
DR InterPro; IPR037652; Mim2.
DR PANTHER; PTHR28230; PTHR28230; 1.
DR Pfam; PF19117; Mim2; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion outer membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..87
FT /note="Mitochondrial import protein 2"
FT /id="PRO_0000247352"
FT TOPO_DOM 1..53
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:22467864"
FT TRANSMEM 54..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..87
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000269|PubMed:22467864"
SQ SEQUENCE 87 AA; 9985 MW; F0BEA00C30B47D27 CRC64;
MADSEDTSVI LQGIDTINSV EGLEEDGYLS DEDTSLSNEL ADAQRQWEES LQQLNKLLNW
VLLPLLGKYI GRRMAKTLWS RFIEHFV
