MIME_BOVIN
ID MIME_BOVIN Reviewed; 299 AA.
AC P19879; O18818;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 2.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Mimecan;
DE AltName: Full=Osteoglycin;
DE Contains:
DE RecName: Full=Corneal keratan sulfate proteoglycan 25 core protein;
DE Short=KSPG25 protein;
DE Contains:
DE RecName: Full=Osteoinductive factor;
DE Short=OIF;
DE Flags: Precursor;
GN Name=OGN; Synonyms=OIF;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2372374; DOI=10.1089/dna.1990.9.303;
RA Madisen L., Neubauer M., Plowman G., Rosen D.M., Segarini P.R., Dasch J.R.,
RA Thompson A.Y., Ziman J., Bentz H., Purchio A.F.;
RT "Molecular cloning of a novel bone-forming compound: osteoinductive
RT factor.";
RL DNA Cell Biol. 9:303-309(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND PROTEIN SEQUENCE OF
RP 77-88.
RC TISSUE=Cornea;
RX PubMed=9346963; DOI=10.1074/jbc.272.44.28089;
RA Funderburgh J.L., Corpuz L.M., Roth M.R., Funderburgh M.L., Tasheva E.S.,
RA Conrad G.W.;
RT "Mimecan, the 25-kDa corneal keratan sulfate proteoglycan, is a product of
RT the gene producing osteoglycin.";
RL J. Biol. Chem. 272:28089-28095(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10373482; DOI=10.1074/jbc.274.26.18693;
RA Tasheva E.S., Funderburgh M.L., McReynolds J., Funderburgh J.L.,
RA Conrad G.W.;
RT "The bovine mimecan gene. Molecular cloning and characterization of two
RT major RNA transcripts generated by alternative use of two splice acceptor
RT sites in the third exon.";
RL J. Biol. Chem. 274:18693-18701(1999).
RN [4]
RP PROTEIN SEQUENCE OF 195-299.
RC TISSUE=Brain;
RX PubMed=2180954; DOI=10.1016/s0021-9258(19)34078-5;
RA Bentz H., Chang R.-J., Thompson A.Y., Glaser C.B., Rosen D.M.;
RT "Amino acid sequence of bovine osteoinductive factor.";
RL J. Biol. Chem. 265:5024-5029(1990).
RN [5]
RP PROTEIN SEQUENCE OF 195-227.
RC TISSUE=Brain;
RX PubMed=2584240; DOI=10.1016/s0021-9258(19)47133-0;
RA Bentz H., Nathan R.M., Rosen D.M., Armstrong R.M., Thompson A.Y.,
RA Segarini P.R., Mathews M.C., Dasch J.R., Piez K.A., Seyedin S.M.;
RT "Purification and characterization of a unique osteoinductive factor from
RT bovine bone.";
RL J. Biol. Chem. 264:20805-20810(1989).
CC -!- FUNCTION: Induces bone formation in conjunction with TGF-beta-1 or TGF-
CC beta-2.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Bone and cornea. {ECO:0000269|PubMed:9346963}.
CC -!- PTM: Sugar residues appear necessary for the activity.
CC -!- PTM: The 12 kDa OIF in bone and the 25 kDa KSPG25 protein in cornea are
CC probably proteolytic fragments.
CC -!- SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP)
CC family. SLRP class III subfamily. {ECO:0000305}.
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DR EMBL; M37974; AAA30670.1; -; mRNA.
DR EMBL; AF017339; AAB70264.1; -; mRNA.
DR EMBL; AF105150; AAD40387.1; -; Genomic_DNA.
DR PIR; A35272; A35272.
DR RefSeq; NP_776371.1; NM_173946.2.
DR AlphaFoldDB; P19879; -.
DR SMR; P19879; -.
DR STRING; 9913.ENSBTAP00000055245; -.
DR iPTMnet; P19879; -.
DR PaxDb; P19879; -.
DR PeptideAtlas; P19879; -.
DR PRIDE; P19879; -.
DR GeneID; 280884; -.
DR KEGG; bta:280884; -.
DR CTD; 4969; -.
DR eggNOG; KOG0619; Eukaryota.
DR InParanoid; P19879; -.
DR OrthoDB; 927103at2759; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR027211; Mimecan.
DR InterPro; IPR043547; Mimecan/Epiphycan.
DR PANTHER; PTHR46269; PTHR46269; 1.
DR PANTHER; PTHR46269:SF1; PTHR46269:SF1; 1.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 4.
DR PROSITE; PS51450; LRR; 4.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Extracellular matrix;
KW Glycoprotein; Growth factor; Leucine-rich repeat; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..299
FT /note="Mimecan"
FT /id="PRO_0000032757"
FT CHAIN 77..299
FT /note="Corneal keratan sulfate proteoglycan 25 core
FT protein"
FT /id="PRO_0000032758"
FT CHAIN 195..299
FT /note="Osteoinductive factor"
FT /evidence="ECO:0000269|PubMed:2180954"
FT /id="PRO_0000032759"
FT REPEAT 113..132
FT /note="LRR 1"
FT REPEAT 133..156
FT /note="LRR 2"
FT REPEAT 157..180
FT /note="LRR 3"
FT REPEAT 181..200
FT /note="LRR 4"
FT REPEAT 201..226
FT /note="LRR 5"
FT REPEAT 227..247
FT /note="LRR 6"
FT REPEAT 248..278
FT /note="LRR 7"
FT CARBOHYD 246
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:2180954"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:2180954"
FT DISULFID 256..289
FT /evidence="ECO:0000269|PubMed:2180954"
FT CONFLICT 79
FT /note="N -> T (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="E -> G (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 299 AA; 34209 MW; B892D3DB18B01C79 CRC64;
MKTLQSTLLL FLFVPLIKPA PPSQQDSRII YDYGTDNLEE TFFSQDYEDK YLDGKSTKEK
ETMIIVPDEK SFQLQKDENI TPLPPKKEND EMPTCLLCVC LSGSVYCEEV DIDAVPPLPK
ESAYLYARFN KIKKLTAKDF ADIPNLRRLD FTGNLIEDIE DGTFSKLSLL EELTLAENQL
LKLPVLPPKL TLFNAKYNKI KSRGIKANTF KKLHNLSFLY LDHNALESVP LNLPESLRVI
HLQFNNITSI TDDTFCKAND TSYIRDRIEE IRLEGNPVIL GKHPNSFICL KRLPIGSYI
