MIME_HUMAN
ID MIME_HUMAN Reviewed; 298 AA.
AC P20774; Q6FIB0; Q9UF90; Q9UNK5;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 202.
DE RecName: Full=Mimecan;
DE AltName: Full=Osteoglycin;
DE AltName: Full=Osteoinductive factor;
DE Short=OIF;
DE Flags: Precursor;
GN Name=OGN; Synonyms=OIF, SLRR3A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2372374; DOI=10.1089/dna.1990.9.303;
RA Madisen L., Neubauer M., Plowman G., Rosen D.M., Segarini P.R., Dasch J.R.,
RA Thompson A.Y., Ziman J., Bentz H., Purchio A.F.;
RT "Molecular cloning of a novel bone-forming compound: osteoinductive
RT factor.";
RL DNA Cell Biol. 9:303-309(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Grover J., Rodriguez E., Roughley P.J.;
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10802664; DOI=10.1038/75664;
RA Pellegata N.S., Dieguez-Lucena J.L., Joensuu T., Lau S., Montgomery K.T.,
RA Krahe R., Kivelae T., Kucherlapati R., Forsius H., de la Chapelle A.;
RT "Mutations in KERA, encoding keratocan, cause cornea plana.";
RL Nat. Genet. 25:91-95(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pituitary;
RX PubMed=10931946; DOI=10.1073/pnas.160270997;
RA Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X.,
RA Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W.,
RA Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J., Xu S.-H., Gu J.,
RA Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z.,
RA Chen M.-D., Chen J.-L.;
RT "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis
RT and full-length cDNA cloning.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
RX PubMed=10373482; DOI=10.1074/jbc.274.26.18693;
RA Tasheva E.S., Funderburgh M.L., McReynolds J., Funderburgh J.L.,
RA Conrad G.W.;
RT "The bovine mimecan gene. Molecular cloning and characterization of two
RT major RNA transcripts generated by alternative use of two splice acceptor
RT sites in the third exon.";
RL J. Biol. Chem. 274:18693-18701(1999).
RN [10]
RP PROTEIN SEQUENCE OF 21-35.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
RN [11]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 156-298.
RC TISSUE=Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [12]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-258.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
RN [13]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-80, AND STRUCTURE OF
RP CARBOHYDRATES.
RC TISSUE=Cerebrospinal fluid;
RX PubMed=19838169; DOI=10.1038/nmeth.1392;
RA Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G.,
RA Larson G.;
RT "Enrichment of glycopeptides for glycan structure and attachment site
RT identification.";
RL Nat. Methods 6:809-811(2009).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Induces bone formation in conjunction with TGF-beta-1 or TGF-
CC beta-2.
CC -!- INTERACTION:
CC P20774; P46108: CRK; NbExp=2; IntAct=EBI-1753690, EBI-886;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Bone.
CC -!- PTM: N- and O-glycosylated. O-glycosylated with a core 1 or possibly
CC core 8 glycan. {ECO:0000269|PubMed:19159218,
CC ECO:0000269|PubMed:19838169}.
CC -!- SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP)
CC family. SLRP class III subfamily. {ECO:0000305}.
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DR EMBL; AF192483; AAF19364.1; -; Genomic_DNA.
DR EMBL; AF192478; AAF19364.1; JOINED; Genomic_DNA.
DR EMBL; AF192479; AAF19364.1; JOINED; Genomic_DNA.
DR EMBL; AF192480; AAF19364.1; JOINED; Genomic_DNA.
DR EMBL; AF192481; AAF19364.1; JOINED; Genomic_DNA.
DR EMBL; AF192482; AAF19364.1; JOINED; Genomic_DNA.
DR EMBL; AF202167; AAF69109.1; -; mRNA.
DR EMBL; AF100758; AAD43022.1; -; mRNA.
DR EMBL; AL110267; CAB53706.1; -; mRNA.
DR EMBL; CR533516; CAG38547.1; -; mRNA.
DR EMBL; AL137848; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC037273; AAH37273.1; -; mRNA.
DR EMBL; BC095443; AAH95443.1; -; mRNA.
DR EMBL; AF112465; AAD40453.1; -; Genomic_DNA.
DR EMBL; AL133114; CAB61417.2; -; mRNA.
DR CCDS; CCDS6695.1; -.
DR PIR; B35272; B35272.
DR RefSeq; NP_054776.1; NM_014057.4.
DR RefSeq; NP_077727.3; NM_024416.4.
DR RefSeq; NP_148935.1; NM_033014.3.
DR AlphaFoldDB; P20774; -.
DR SMR; P20774; -.
DR BioGRID; 111019; 13.
DR IntAct; P20774; 9.
DR STRING; 9606.ENSP00000262551; -.
DR GlyConnect; 767; 13 N-Linked glycans (1 site), 1 O-Linked glycan (1 site).
DR GlyGen; P20774; 3 sites, 12 N-linked glycans (1 site), 2 O-linked glycans (1 site).
DR iPTMnet; P20774; -.
DR PhosphoSitePlus; P20774; -.
DR BioMuta; OGN; -.
DR DMDM; 129078; -.
DR REPRODUCTION-2DPAGE; IPI00025465; -.
DR jPOST; P20774; -.
DR MassIVE; P20774; -.
DR MaxQB; P20774; -.
DR PaxDb; P20774; -.
DR PeptideAtlas; P20774; -.
DR PRIDE; P20774; -.
DR ProteomicsDB; 53784; -.
DR Antibodypedia; 2122; 309 antibodies from 34 providers.
DR DNASU; 4969; -.
DR Ensembl; ENST00000262551.8; ENSP00000262551.4; ENSG00000106809.11.
DR Ensembl; ENST00000375561.10; ENSP00000364711.5; ENSG00000106809.11.
DR GeneID; 4969; -.
DR KEGG; hsa:4969; -.
DR MANE-Select; ENST00000375561.10; ENSP00000364711.5; NM_014057.5; NP_054776.1.
DR UCSC; uc004asa.4; human.
DR CTD; 4969; -.
DR DisGeNET; 4969; -.
DR GeneCards; OGN; -.
DR HGNC; HGNC:8126; OGN.
DR HPA; ENSG00000106809; Tissue enhanced (cervix).
DR MIM; 602383; gene.
DR neXtProt; NX_P20774; -.
DR OpenTargets; ENSG00000106809; -.
DR PharmGKB; PA31913; -.
DR VEuPathDB; HostDB:ENSG00000106809; -.
DR eggNOG; KOG0619; Eukaryota.
DR GeneTree; ENSGT00940000157238; -.
DR HOGENOM; CLU_067583_1_0_1; -.
DR InParanoid; P20774; -.
DR OMA; RILHLQY; -.
DR OrthoDB; 927103at2759; -.
DR PhylomeDB; P20774; -.
DR TreeFam; TF351924; -.
DR PathwayCommons; P20774; -.
DR Reactome; R-HSA-2022854; Keratan sulfate biosynthesis.
DR Reactome; R-HSA-2022857; Keratan sulfate degradation.
DR Reactome; R-HSA-3656225; Defective CHST6 causes MCDC1.
DR Reactome; R-HSA-3656243; Defective ST3GAL3 causes MCT12 and EIEE15.
DR Reactome; R-HSA-3656244; Defective B4GALT1 causes B4GALT1-CDG (CDG-2d).
DR SignaLink; P20774; -.
DR BioGRID-ORCS; 4969; 10 hits in 1066 CRISPR screens.
DR ChiTaRS; OGN; human.
DR GeneWiki; OGN_(gene); -.
DR GenomeRNAi; 4969; -.
DR Pharos; P20774; Tbio.
DR PRO; PR:P20774; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; P20774; protein.
DR Bgee; ENSG00000106809; Expressed in right coronary artery and 174 other tissues.
DR ExpressionAtlas; P20774; baseline and differential.
DR Genevisible; P20774; HS.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; HDA:BHF-UCL.
DR GO; GO:0005615; C:extracellular space; HDA:BHF-UCL.
DR GO; GO:1903561; C:extracellular vesicle; HDA:UniProtKB.
DR GO; GO:0005796; C:Golgi lumen; TAS:Reactome.
DR GO; GO:0043202; C:lysosomal lumen; TAS:Reactome.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0061975; P:articular cartilage development; IBA:GO_Central.
DR GO; GO:0060348; P:bone development; IBA:GO_Central.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISS:UniProtKB.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR027211; Mimecan.
DR InterPro; IPR043547; Mimecan/Epiphycan.
DR PANTHER; PTHR46269; PTHR46269; 1.
DR PANTHER; PTHR46269:SF1; PTHR46269:SF1; 1.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 4.
DR PROSITE; PS51450; LRR; 4.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Extracellular matrix;
KW Glycoprotein; Growth factor; Leucine-rich repeat; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 21..298
FT /note="Mimecan"
FT /id="PRO_0000032760"
FT REPEAT 112..131
FT /note="LRR 1"
FT REPEAT 132..155
FT /note="LRR 2"
FT REPEAT 156..179
FT /note="LRR 3"
FT REPEAT 180..199
FT /note="LRR 4"
FT REPEAT 200..225
FT /note="LRR 5"
FT REPEAT 226..246
FT /note="LRR 6"
FT REPEAT 247..277
FT /note="LRR 7"
FT CARBOHYD 80
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:19838169"
FT CARBOHYD 214
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 258
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218"
FT DISULFID 255..288
FT /evidence="ECO:0000250"
SQ SEQUENCE 298 AA; 33922 MW; F4590785C3849F83 CRC64;
MKTLQSTLLL LLLVPLIKPA PPTQQDSRII YDYGTDNFEE SIFSQDYEDK YLDGKNIKEK
ETVIIPNEKS LQLQKDEAIT PLPPKKENDE MPTCLLCVCL SGSVYCEEVD IDAVPPLPKE
SAYLYARFNK IKKLTAKDFA DIPNLRRLDF TGNLIEDIED GTFSKLSLLE ELSLAENQLL
KLPVLPPKLT LFNAKYNKIK SRGIKANAFK KLNNLTFLYL DHNALESVPL NLPESLRVIH
LQFNNIASIT DDTFCKANDT SYIRDRIEEI RLEGNPIVLG KHPNSFICLK RLPIGSYF
