ID   MIMR_MYCS2              Reviewed;         583 AA.
AC   A0QTU7;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Propane 2-monooxygenase operon transcriptional activator MimR {ECO:0000303|PubMed:21856847};
GN   Name=mimR {ECO:0000303|PubMed:21856847};
GN   OrderedLocusNames=MSMEG_1970 {ECO:0000312|EMBL:ABK75314.1};
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155 {ECO:0000312|Proteomes:UP000000757};
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=21856847; DOI=10.1128/jb.05525-11;
RA   Furuya T., Hirose S., Semba H., Kino K.;
RT   "Identification of the regulator gene responsible for the acetone-
RT   responsive expression of the binuclear iron monooxygenase gene cluster in
RT   mycobacteria.";
RL   J. Bacteriol. 193:5817-5823(2011).
CC   -!- FUNCTION: Acts as a transcriptional activator of the mimABCD operon
CC       encoding the propane 2-monooxygenase complex.
CC       {ECO:0000269|PubMed:21856847}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are unable to express the
CC       mimABCD operon. {ECO:0000269|PubMed:21856847}.
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DR   EMBL; CP000480; ABK75314.1; -; Genomic_DNA.
DR   RefSeq; WP_011728053.1; NZ_SIJM01000020.1.
DR   RefSeq; YP_886335.1; NC_008596.1.
DR   AlphaFoldDB; A0QTU7; -.
DR   SMR; A0QTU7; -.
DR   STRING; 246196.MSMEI_1926; -.
DR   EnsemblBacteria; ABK75314; ABK75314; MSMEG_1970.
DR   GeneID; 66733400; -.
DR   KEGG; msm:MSMEG_1970; -.
DR   PATRIC; fig|246196.19.peg.1947; -.
DR   eggNOG; COG3284; Bacteria.
DR   OMA; HVEVTFE; -.
DR   OrthoDB; 123059at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   Pfam; PF02954; HTH_8; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Nucleotide-binding;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..583
FT                   /note="Propane 2-monooxygenase operon transcriptional
FT                   activator MimR"
FT                   /id="PRO_0000442976"
FT   DOMAIN          320..513
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         349..356
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         395..404
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   583 AA;  64130 MW;  A9C1CFBA55F16885 CRC64;
     MVRHADTPTD AQRAREQFLS TGALQPDAVA SSVLNSWQRS RELHVHPDRV ELPYVRDPDT
     DTPLMHAAAP VLRRMAEDLS DQSVSVVLTS ADGLVLDRVA ADTEFERVLD DVRLARGYSY
     AEEFAGTNGI GTALETGRPA FIRGSEHYVG TLGGLACAGS PIREPVTRRI LGVIDLTCRA
     RQADPLLFVL AKSAGSQIED RLRTMNNETE TALLDAYLKQ SRRYPGGVLA IGGDVVLMNR
     YLRQALDAAD QTVLLDHAAE LTRSSFTSTT VAHLPSGASV KISAAERIIV GVRSDSVVFH
     VSVHVAESIP VRSSQHIPRL AGQSSSFRRS AQQVERCYRD REWVVIEGER GSGRTYLGYS
     VVHFVTPERS IPVLHIDNFD TPESFVAAFE SETDSADFAV IVSDVDELPD EVLNPMAAIM
     QTRAGRGWIA ATTSTERDSQ LVDLLMLPFF THTVTVPALR HRIEDLQELV PMILNELSRG
     EARMDGEAMR QLAKLPWPGN IAQLRHVLTE TVRRQRSGVI GVDKLPAECR SVTRRKLTPL
     EAMERDAIVR SLLENDGNKA DAAAALGMSR ATIYRKIKDF GIA