MINA1_CAEEL
ID MINA1_CAEEL Reviewed; 393 AA.
AC Q93367;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Messenger RNA-binding inhibitor of apoptosis 1 {ECO:0000303|PubMed:30728462};
GN Name=mina-1 {ECO:0000303|PubMed:30728462};
GN ORFNames=C41G7.3 {ECO:0000312|WormBase:C41G7.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0007744|PDB:6FBL}
RP STRUCTURE BY NMR OF 254-334, FUNCTION, INTERACTION WITH WAGO-4, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DOMAIN, DISRUPTION PHENOTYPE, AND MUTAGENESIS
RP OF 276-ASN-ARG-277.
RX PubMed=30728462; DOI=10.1038/s41418-019-0291-z;
RA Sendoel A., Subasic D., Ducoli L., Keller M., Michel E., Kohler I.,
RA Singh K.D., Zheng X., Bruemmer A., Imig J., Kishore S., Wu Y., Kanitz A.,
RA Kaech A., Mittal N., Matia-Gonzalez A.M., Gerber A.P., Zavolan M.,
RA Aebersold R., Hall J., Allain F.H., Hengartner M.O.;
RT "MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell
RT death and RNAi in C. elegans.";
RL Cell Death Differ. 26:2157-2178(2019).
CC -!- FUNCTION: RNA-binding protein which binds to its own mRNA and target
CC mRNAs to negatively regulate gene expression to modulate apoptosis and
CC differentiation in the germline (PubMed:30728462). Negatively regulates
CC the expression of the argonaute protein wago-4, and may thus play a
CC role in RNA-mediated gene silencing (RNAi) in the germline
CC (PubMed:30728462). {ECO:0000269|PubMed:30728462}.
CC -!- SUBUNIT: May interact with wago-4. {ECO:0000269|PubMed:30728462}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:30728462}.
CC -!- TISSUE SPECIFICITY: Expressed throughout the germline and in oocytes
CC (at protein level). {ECO:0000269|PubMed:30728462}.
CC -!- DOMAIN: The KH-like 3 domain is required for binding to RNA.
CC {ECO:0000269|PubMed:30728462}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in increased
CC apoptosis and increased sensitivity to DNA damage-induced apoptosis in
CC response to ionizing radiation in the germline.
CC {ECO:0000269|PubMed:30728462}.
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DR EMBL; BX284601; CAB02840.2; -; Genomic_DNA.
DR RefSeq; NP_492528.2; NM_060127.5.
DR PDB; 6FBL; NMR; -; A=254-334.
DR PDBsum; 6FBL; -.
DR AlphaFoldDB; Q93367; -.
DR BMRB; Q93367; -.
DR SMR; Q93367; -.
DR STRING; 6239.C41G7.3; -.
DR EPD; Q93367; -.
DR PaxDb; Q93367; -.
DR EnsemblMetazoa; C41G7.3.1; C41G7.3.1; WBGene00008061.
DR GeneID; 172786; -.
DR KEGG; cel:CELE_C41G7.3; -.
DR UCSC; C41G7.3; c. elegans.
DR CTD; 172786; -.
DR WormBase; C41G7.3; CE42810; WBGene00008061; mina-1.
DR eggNOG; ENOG502TG75; Eukaryota.
DR HOGENOM; CLU_700640_0_0_1; -.
DR InParanoid; Q93367; -.
DR OMA; ITVHTEV; -.
DR OrthoDB; 1096930at2759; -.
DR PRO; PR:Q93367; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00008061; Expressed in germ line (C elegans) and 3 other tissues.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IMP:UniProtKB.
DR Gene3D; 3.30.1370.10; -; 1.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR Pfam; PF00013; KH_1; 1.
DR SMART; SM00322; KH; 2.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; RNA-binding.
FT CHAIN 1..393
FT /note="Messenger RNA-binding inhibitor of apoptosis 1"
FT /id="PRO_0000448350"
FT REGION 12..76
FT /note="KH 1-like"
FT /evidence="ECO:0000305|PubMed:30728462"
FT REGION 79..157
FT /note="KH 2-like"
FT /evidence="ECO:0000305|PubMed:30728462"
FT REGION 259..322
FT /note="KH 3-like"
FT /evidence="ECO:0000269|PubMed:30728462,
FT ECO:0007744|PDB:6FBL"
FT REGION 328..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..363
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 276..277
FT /note="NR->DD: Impairs RNA-binding."
FT /evidence="ECO:0000269|PubMed:30728462"
FT STRAND 259..265
FT /evidence="ECO:0007829|PDB:6FBL"
FT HELIX 267..270
FT /evidence="ECO:0007829|PDB:6FBL"
FT HELIX 271..274
FT /evidence="ECO:0007829|PDB:6FBL"
FT HELIX 279..288
FT /evidence="ECO:0007829|PDB:6FBL"
FT STRAND 291..294
FT /evidence="ECO:0007829|PDB:6FBL"
FT STRAND 305..313
FT /evidence="ECO:0007829|PDB:6FBL"
FT HELIX 314..321
FT /evidence="ECO:0007829|PDB:6FBL"
FT TURN 322..330
FT /evidence="ECO:0007829|PDB:6FBL"
FT HELIX 331..333
FT /evidence="ECO:0007829|PDB:6FBL"
SQ SEQUENCE 393 AA; 43736 MW; 792E48CE9E952DDD CRC64;
MDDSTPYPVP QELYIPQKMK AFMAEPQGCA LVAALEGQFQ CSIVVINDHL SVISSADGVA
VDINQIEKIL RDVWRKRDVQ IMIREAALNA SCTHICHTLL PRAYCAVVLF FSSDLQRRSR
CTDIIIDQFT GKVTMFGTEQ AVNKAREMMI ECLTEHFGLL EMNIPPTQRT TRMGYTNSYN
PEIRTHLPPN SFLNSVFPMG EPNAILTSTP PTTSIMDEPL LSASLEKHLL FPSDFSVPPP
RLSPVQELPL TPPKTCVVEK IKQWIPTTEV GKILGNRAAV KKHIERQFNC VITVHTEVQS
SFGATPVEIV AQNKEQCQEA RNAVMSLMQS HQDKPASNPP DSGFSTPGSP FTSDSSSTTP
EKRGNSRQYH RGSFRDQPKV MLALTPRKLS PSD
