ID   MINY3_BOVIN             Reviewed;         445 AA.
AC   Q0IIH8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Ubiquitin carboxyl-terminal hydrolase MINDY-3;
DE            EC=3.4.19.12;
DE   AltName: Full=Deubiquitinating enzyme MINDY-3;
DE   AltName: Full=Protein CARP;
GN   Name=MINDY3; Synonyms=CARP, FAM188A;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Basal ganglia;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Hydrolase that can remove 'Lys-48'-linked conjugated
CC       ubiquitin from proteins. {ECO:0000250|UniProtKB:Q9H8M7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q9H8M7};
CC   -!- SUBUNIT: Interacts with COPS5. {ECO:0000250|UniProtKB:Q9H8M7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H8M7}.
CC   -!- SIMILARITY: Belongs to the MINDY deubiquitinase family. FAM188
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BC122636; AAI22637.1; -; mRNA.
DR   RefSeq; NP_001069575.1; NM_001076107.1.
DR   AlphaFoldDB; Q0IIH8; -.
DR   STRING; 9913.ENSBTAP00000013714; -.
DR   PaxDb; Q0IIH8; -.
DR   PRIDE; Q0IIH8; -.
DR   Ensembl; ENSBTAT00000013714; ENSBTAP00000013714; ENSBTAG00000010387.
DR   GeneID; 538509; -.
DR   KEGG; bta:538509; -.
DR   CTD; 80013; -.
DR   VEuPathDB; HostDB:ENSBTAG00000010387; -.
DR   VGNC; VGNC:31478; MINDY3.
DR   eggNOG; KOG2871; Eukaryota.
DR   GeneTree; ENSGT00940000155958; -.
DR   HOGENOM; CLU_033478_0_0_1; -.
DR   InParanoid; Q0IIH8; -.
DR   OMA; HVWDHDQ; -.
DR   OrthoDB; 1276386at2759; -.
DR   TreeFam; TF323996; -.
DR   Proteomes; UP000009136; Chromosome 13.
DR   Bgee; ENSBTAG00000010387; Expressed in oocyte and 108 other tissues.
DR   GO; GO:0031965; C:nuclear membrane; IEA:Ensembl.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:1990380; F:Lys48-specific deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR025257; MINDY-3/4_CD.
DR   InterPro; IPR039785; MINY3/4.
DR   PANTHER; PTHR12473; PTHR12473; 1.
DR   Pfam; PF13898; DUF4205; 1.
DR   SMART; SM01174; DUF4205; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Hydrolase; Nucleus; Phosphoprotein; Protease;
KW   Reference proteome; Thiol protease; Ubl conjugation pathway.
FT   CHAIN           1..445
FT                   /note="Ubiquitin carboxyl-terminal hydrolase MINDY-3"
FT                   /id="PRO_0000317559"
FT   ACT_SITE        51
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT   ACT_SITE        287
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT   MOD_RES         125
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H8M7"
SQ   SEQUENCE   445 AA;  49678 MW;  7C0FF05D75F0AC66 CRC64;
     MSELTKELME LVWGTNSSPG LSDTIFCRWT QGFVFSESEG SALEQFEGGP CAVIAPVQAF
     LLKKLLFSSE KSSWRDCPEE ERKELLCHTL CDILESAGCD NSGSYCLVSW LRGKTTEETA
     SLSGSPAQSS CQVEHSSALA VEELGFERFH ALIQKRSFRS LAELRDAVLD QYSMWGNKFG
     VLLFLYSVLL TKGIENIKNE IEDSSEPLID PVYGHGSQSL INLLLTGHAV SNVWDGDREC
     SGMKLLGIHE QAAVGFLTLM EALRYCKVGS YLKSPKFPIW IVGSETHLTV FFAKDMALVA
     PEAPSEQARR VFQTYDPEDN GFIPDSLLED VMKALDLVSD PEYINLMKNK LDPEGLGIIL
     LGPFLQEFFP DQGSSGPESF TVYHYNGLKQ SNYNEKVMYV EGTAVVMGFE DPLLQTDDTP
     IKRCLQTKWP YIELLWTTDR SPSLN