ID   MINY3_XENTR             Reviewed;         441 AA.
AC   Q6NX27;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Ubiquitin carboxyl-terminal hydrolase MINDY-3;
DE            EC=3.4.19.12;
DE   AltName: Full=Deubiquitinating enzyme MINDY-3;
DE   AltName: Full=Protein CARP;
GN   Name=mindy3; Synonyms=carp, fam188a;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Hydrolase that can remove 'Lys-48'-linked conjugated
CC       ubiquitin from proteins. {ECO:0000250|UniProtKB:Q9H8M7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q9H8M7};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H8M7}.
CC   -!- SIMILARITY: Belongs to the MINDY deubiquitinase family. FAM188
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BC067311; AAH67311.1; -; mRNA.
DR   RefSeq; NP_001001204.1; NM_001001204.1.
DR   RefSeq; XP_017950090.1; XM_018094601.1.
DR   AlphaFoldDB; Q6NX27; -.
DR   STRING; 8364.ENSXETP00000062860; -.
DR   PaxDb; Q6NX27; -.
DR   DNASU; 407862; -.
DR   GeneID; 407862; -.
DR   KEGG; xtr:407862; -.
DR   CTD; 80013; -.
DR   Xenbase; XB-GENE-957056; mindy3.
DR   eggNOG; KOG2871; Eukaryota.
DR   InParanoid; Q6NX27; -.
DR   OrthoDB; 1276386at2759; -.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000020297; Expressed in brain and 12 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:1990380; F:Lys48-specific deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR025257; MINDY-3/4_CD.
DR   InterPro; IPR039785; MINY3/4.
DR   PANTHER; PTHR12473; PTHR12473; 1.
DR   Pfam; PF13898; DUF4205; 1.
DR   SMART; SM01174; DUF4205; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Hydrolase; Nucleus; Protease; Reference proteome;
KW   Thiol protease; Ubl conjugation pathway.
FT   CHAIN           1..441
FT                   /note="Ubiquitin carboxyl-terminal hydrolase MINDY-3"
FT                   /id="PRO_0000317564"
FT   ACT_SITE        51
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT   ACT_SITE        284
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N5J2"
SQ   SEQUENCE   441 AA;  49840 MW;  B745F9F088B77FA2 CRC64;
     MSECDKELVD MVWGRNNSNG LADSVFKRWT QGFVFSASEP TALEQFEGGP CAVLAPVQAF
     LLKRQLFNTE HSNWRSCQDE EQKEILCHTL SDILEIVSFN SNSYCLASWL KEKATRETEQ
     ENPAESSQQN EQPTALAAEE LGFERFHASI QKRKFNSLSE LKEAVLETYS TWRNKYGVLL
     FLYSVILTKG IENVKNEIED AERPLIDPVY GHGSQSLINL LLTGHAVSNV WDGDRECSGM
     KLQGIHSHAD VGFLTILESL RFCKVGSFLK SPKFPIWVIG SETHLTVFFT KEMALVAPEA
     PSEQARRVFE TYDPEDNGFI PDAVLEDVMK ALDLVSDTDY VNLMKTKLDP EGLGIILLGP
     FLLEFFPEQS SKVPESFTVY HYNGLRQSNH NEKVAYIEGT AVMGFEDPRL QTDDTPVKCC
     LQTKWPFVEL LWSADRPPSL I