MINY4_HUMAN
ID MINY4_HUMAN Reviewed; 757 AA.
AC Q4G0A6; Q71AZ7; Q9H6D2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable ubiquitin carboxyl-terminal hydrolase MINDY-4;
DE EC=3.4.19.12;
DE AltName: Full=Probable deubiquitinating enzyme MINDY-4;
GN Name=MINDY4 {ECO:0000312|HGNC:HGNC:21916}; Synonyms=C7orf67, FAM188B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Ding P., Han W., Xia D., Li X., Song Q., Zhang Y., Ma D.;
RL Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT MET-322.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT MET-322.
RC TISSUE=Chondrosarcoma, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-451, AND VARIANT MET-322.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219; SER-223 AND SER-289, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Probable hydrolase that can remove 'Lys-48'-linked conjugated
CC ubiquitin from proteins. {ECO:0000250|UniProtKB:Q8NBR6}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q8NBR6};
CC -!- SIMILARITY: Belongs to the MINDY deubiquitinase family. FAM188
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ10898.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB15327.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; AF538962; AAQ10898.1; ALT_FRAME; mRNA.
DR EMBL; AC004691; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471073; EAW93970.1; -; Genomic_DNA.
DR EMBL; BC098569; AAH98569.1; -; mRNA.
DR EMBL; AK026027; BAB15327.1; ALT_SEQ; mRNA.
DR CCDS; CCDS43565.1; -.
DR RefSeq; NP_115598.2; NM_032222.2.
DR AlphaFoldDB; Q4G0A6; -.
DR BioGRID; 123932; 71.
DR IntAct; Q4G0A6; 1.
DR STRING; 9606.ENSP00000265299; -.
DR iPTMnet; Q4G0A6; -.
DR PhosphoSitePlus; Q4G0A6; -.
DR BioMuta; MINDY4; -.
DR DMDM; 296434499; -.
DR EPD; Q4G0A6; -.
DR jPOST; Q4G0A6; -.
DR MassIVE; Q4G0A6; -.
DR MaxQB; Q4G0A6; -.
DR PaxDb; Q4G0A6; -.
DR PeptideAtlas; Q4G0A6; -.
DR PRIDE; Q4G0A6; -.
DR ProteomicsDB; 62101; -.
DR Antibodypedia; 3440; 42 antibodies from 13 providers.
DR DNASU; 84182; -.
DR Ensembl; ENST00000265299.6; ENSP00000265299.6; ENSG00000106125.14.
DR GeneID; 84182; -.
DR KEGG; hsa:84182; -.
DR MANE-Select; ENST00000265299.6; ENSP00000265299.6; NM_032222.3; NP_115598.2.
DR UCSC; uc003tbt.3; human.
DR CTD; 84182; -.
DR DisGeNET; 84182; -.
DR GeneCards; MINDY4; -.
DR HGNC; HGNC:21916; MINDY4.
DR HPA; ENSG00000106125; Low tissue specificity.
DR neXtProt; NX_Q4G0A6; -.
DR OpenTargets; ENSG00000106125; -.
DR PharmGKB; PA165618038; -.
DR VEuPathDB; HostDB:ENSG00000106125; -.
DR eggNOG; KOG2871; Eukaryota.
DR GeneTree; ENSGT00940000159600; -.
DR HOGENOM; CLU_011769_1_0_1; -.
DR InParanoid; Q4G0A6; -.
DR OMA; QFESGPF; -.
DR OrthoDB; 1276386at2759; -.
DR PhylomeDB; Q4G0A6; -.
DR TreeFam; TF323996; -.
DR PathwayCommons; Q4G0A6; -.
DR BioGRID-ORCS; 84182; 10 hits in 1109 CRISPR screens.
DR GeneWiki; C7orf67; -.
DR GenomeRNAi; 84182; -.
DR Pharos; Q4G0A6; Tdark.
DR PRO; PR:Q4G0A6; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q4G0A6; protein.
DR Bgee; ENSG00000106125; Expressed in right uterine tube and 98 other tissues.
DR Genevisible; Q4G0A6; HS.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR GO; GO:1990380; F:Lys48-specific deubiquitinase activity; IBA:GO_Central.
DR InterPro; IPR025257; MINDY-3/4_CD.
DR InterPro; IPR039785; MINY3/4.
DR PANTHER; PTHR12473; PTHR12473; 1.
DR Pfam; PF13898; DUF4205; 1.
DR SMART; SM01174; DUF4205; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease;
KW Ubl conjugation pathway.
FT CHAIN 1..757
FT /note="Probable ubiquitin carboxyl-terminal hydrolase
FT MINDY-4"
FT /id="PRO_0000320590"
FT REGION 152..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..245
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..318
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 456
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT ACT_SITE 677
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8N5J2"
FT MOD_RES 219
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 223
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 289
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 183
FT /note="D -> A (in dbSNP:rs34357272)"
FT /id="VAR_039212"
FT VARIANT 262
FT /note="S -> L (in dbSNP:rs17159453)"
FT /id="VAR_039213"
FT VARIANT 322
FT /note="T -> M (in dbSNP:rs12701034)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3"
FT /id="VAR_039214"
FT VARIANT 324
FT /note="R -> K (in dbSNP:rs35897481)"
FT /id="VAR_039215"
FT VARIANT 355
FT /note="A -> V (in dbSNP:rs12672119)"
FT /id="VAR_039216"
FT VARIANT 465
FT /note="C -> Y (in dbSNP:rs34970833)"
FT /id="VAR_062248"
FT VARIANT 655
FT /note="M -> V (in dbSNP:rs10216063)"
FT /id="VAR_039217"
FT CONFLICT 246
FT /note="R -> G (in Ref. 1; AAQ10898)"
FT /evidence="ECO:0000305"
FT CONFLICT 483
FT /note="G -> A (in Ref. 1; AAQ10898)"
FT /evidence="ECO:0000305"
FT CONFLICT 632
FT /note="I -> F (in Ref. 1; AAQ10898)"
FT /evidence="ECO:0000305"
FT CONFLICT 647
FT /note="L -> F (in Ref. 1; AAQ10898)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 757 AA; 84372 MW; 1DAB6CAE92042C8E CRC64;
MDSLFVEEVA ASLVREFLSR KGLKKTCVTM DQERPRSDLS INNRNDLRKV LHLEFLYKEN
KAKENPLKTS LELITRYFLD HFGNTANNFT QDTPIPALSV PKKNNKVPSR CSETTLVNIY
DLSDEDAGWR TSLSETSKAR HDNLDGDVLG NFVSSKRPPH KSKPMQTVPG ETPVLTSAWE
KIDKLHSEPS LDVKRMGENS RPKSGLIVRG MMSGPIASSP QDSFHRHYLR RSSPSSSSTQ
PQEESRKVPE LFVCTQQDIL ASSNSSPSRT SLGQLSELTV ERQKTTASSP PHLPSKRLPP
WDRARPRDPS EDTPAVDGST DTDRMPLKLY LPGGNSRMTQ ERLERAFKRQ GSQPAPVRKN
QLLPSDKVDG ELGALRLEDV EDELIREEVI LSPVPSVLKL QTASKPIDLS VAKEIKTLLF
GSSFCCFNEE WKLQSFSFSN TASLKYGIVQ NKGGPCGVLA AVQGCVLQKL LFEGDSKADC
AQGLQPSDAH RTRCLVLALA DIVWRAGGRE RAVVALASRT QQFSPTGKYK ADGVLETLTL
HSLTCYEDLV TFLQQSIHQF EVGPYGCILL TLSAILSRST ELIRQDFDVP TSHLIGAHGY
CTQELVNLLL TGKAVSNVFN DVVELDSGDG NITLLRGIAA RSDIGFLSLF EHYNMCQVGC
FLKTPRFPIW VVCSESHFSI LFSLQPGLLR DWRTERLFDL YYYDGLANQQ EQIRLTIDTT
QTISEDTDND LVPPLELCIR TKWKGASVNW NGSDPIL
