ID   MIO_DICDI               Reviewed;        1119 AA.
AC   Q551B5;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=GATOR complex protein MIOS {ECO:0000305};
GN   Name=mios {ECO:0000305}; ORFNames=DDB_G0276673;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: As a component of the GATOR complex may function in the amino
CC       acid-sensing branch of the TORC1 signaling pathway.
CC       {ECO:0000250|UniProtKB:Q9NXC5}.
CC   -!- SUBUNIT: Probably part of the GATOR complex.
CC       {ECO:0000250|UniProtKB:Q9NXC5}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9NXC5}.
CC   -!- SIMILARITY: Belongs to the WD repeat mio family. {ECO:0000305}.
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DR   EMBL; AAFI02000018; EAL69094.1; -; Genomic_DNA.
DR   RefSeq; XP_643023.1; XM_637931.1.
DR   AlphaFoldDB; Q551B5; -.
DR   SMR; Q551B5; -.
DR   STRING; 44689.DDB0202944; -.
DR   PaxDb; Q551B5; -.
DR   EnsemblProtists; EAL69094; EAL69094; DDB_G0276673.
DR   GeneID; 8620627; -.
DR   KEGG; ddi:DDB_G0276673; -.
DR   dictyBase; DDB_G0276673; -.
DR   eggNOG; KOG1008; Eukaryota.
DR   HOGENOM; CLU_005843_0_0_1; -.
DR   InParanoid; Q551B5; -.
DR   OMA; QSHNSTC; -.
DR   PhylomeDB; Q551B5; -.
DR   Reactome; R-DDI-9639288; Amino acids regulate mTORC1.
DR   PRO; PR:Q551B5; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd16691; mRING-H2-C3H3C2_Mio; 1.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR037593; MIOS/Sea4.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR031488; Zn_ribbon_mio.
DR   PANTHER; PTHR16453; PTHR16453; 1.
DR   Pfam; PF17034; zinc_ribbon_16; 1.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Lysosome; Membrane; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..1119
FT                   /note="GATOR complex protein MIOS"
FT                   /id="PRO_0000329411"
FT   REPEAT          3..43
FT                   /note="WD 1"
FT   REPEAT          127..169
FT                   /note="WD 2"
FT   REPEAT          183..227
FT                   /note="WD 3"
FT   REPEAT          281..321
FT                   /note="WD 4"
FT   REPEAT          324..364
FT                   /note="WD 5"
FT   REPEAT          368..409
FT                   /note="WD 6"
FT   REPEAT          459..499
FT                   /note="WD 7"
FT   REGION          41..60
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          413..455
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          601..669
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        45..60
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1119 AA;  126106 MW;  85C0EA144BABF742 CRC64;
     MSQSSTRKRL IQWSPHNKSS FIVGSNDLRL YNFKFKDKNE KKNENNINNS NQYNQNNQQQ
     QQQQIQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQHDIYSPK SITSKYMETY NFDIEYPPDD
     WVPQEKKTIS LMSVNSDVQL MKCMAWCPDE SDTNLIACGL TSGRAILTSF SSVNRILKEL
     VPKHTRSCNA ISWNPIYTNQ LAVGLDKVRG DSSTLIWDIN YLQSTTLNNH LHKNNNNYIS
     KLQQQQNNID QPYIISQISD NSFSTSTDYT DTIYQPISEF TQSEATLALA WLPNNPSCLL
     VGTGSKWLKL YDIRDVNSSQ SVMAHQKSVN GVCVDPFDFN RIATMSEDSH IKVWDLRNLD
     DPLIIVNSNC KSIQQIDWCP TRSGVLASVG KDKSSIKLWD IKAPIEFSKS PKLESTTLST
     GGGGSGSNTS NNLNKRSTSN NNNSQDPINT ISKPTKIHHS SDVVSSFSWH PTNECRMLTV
     SYSGVIDVVS LNENIPISWS PHGGICFSFG NNVLEGPTKG ETLEPNILNY KNLINDGRYE
     KDISSKIKER AIFGYSANVE ENINLANKIN DENINFLWKW IQKVPIQIQN FKRESSLIST
     PSISTTTPGG PITPSATTKT IINNNNNNNN NKNINNNINN INNNNNNNNN NNNNNNNNNN
     NNNNNNNNNN EYNGIYNILI EKDISTVESS NGFVIYKSPN RNLCLSICGW GFNQSLPLEN
     ILSRLEKSGE YERAAAIAVF HLDIKRAILV VTNATYNIHP VTQTNHHGHL MRQDREFSLK
     LLSIALAGFD GSSTSNNNNN NNNNNNNIWK ETCKSTAKSF INPYLKTCLE FLASNSDSKD
     IYSIIEDSRI NLDDKIAFSC KYLDYQDLIH FVERNTIRVI ECGNLKGVLL TGLTGRGVDL
     LSNYIDRTCD IQTAVLAISL VVPKFFRDKR VSKWSSIYSD LLDQWELWHE RAILDIQTRI
     SGIDQPPTPQ IFAKCGFCQN SFAFESISAS SIVGRNASSK PNFKAKVPFC PHCKQSLPRC
     CLCLLPLNCM VPTPEFKKSN TTIGSGGGSI ASSGGINNSG SINNPNDSQL WSNGSEPFED
     WFTWCQTCRH GGHSQHILDW FKDHSICPVT SCDCRCSQL