MIO_MOUSE
ID MIO_MOUSE Reviewed; 875 AA.
AC Q8VE19; E9QM00;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=GATOR complex protein MIOS {ECO:0000305};
GN Name=Mios {ECO:0000312|MGI:MGI:2182066};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-766, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: As a component of the GATOR subcomplex GATOR2, functions
CC within the amino acid-sensing branch of the TORC1 signaling pathway.
CC Indirectly activates mTORC1 and the TORC1 signaling pathway through the
CC inhibition of the GATOR1 subcomplex. It is negatively regulated by the
CC upstream amino acid sensors SESN2 and CASTOR1.
CC {ECO:0000250|UniProtKB:Q9NXC5}.
CC -!- SUBUNIT: Within the GATOR complex, component of the GATOR2 subcomplex,
CC made of MIOS, SEC13, SEH1L, WDR24 and WDR59. The GATOR complex strongly
CC interacts with RRAGA/RRAGC and RRAGB/RRAGC heterodimers. The GATOR2
CC complex interacts with CASTOR2 and CASTOR1; the interaction is
CC negatively regulated by arginine. The GATOR2 complex interacts with the
CC sestrins SESN1, SESN2 and SESN3; the interaction is negatively
CC regulated by amino acids. {ECO:0000250|UniProtKB:Q9NXC5}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9NXC5}.
CC -!- SIMILARITY: Belongs to the WD repeat mio family. {ECO:0000305}.
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DR EMBL; AC158661; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC020002; AAH20002.1; -; mRNA.
DR CCDS; CCDS39425.1; -.
DR RefSeq; NP_663349.2; NM_145374.2.
DR RefSeq; XP_006505149.1; XM_006505086.3.
DR AlphaFoldDB; Q8VE19; -.
DR SMR; Q8VE19; -.
DR BioGRID; 232968; 15.
DR IntAct; Q8VE19; 2.
DR MINT; Q8VE19; -.
DR STRING; 10090.ENSMUSP00000039301; -.
DR iPTMnet; Q8VE19; -.
DR PhosphoSitePlus; Q8VE19; -.
DR EPD; Q8VE19; -.
DR jPOST; Q8VE19; -.
DR MaxQB; Q8VE19; -.
DR PaxDb; Q8VE19; -.
DR PeptideAtlas; Q8VE19; -.
DR PRIDE; Q8VE19; -.
DR ProteomicsDB; 290251; -.
DR Antibodypedia; 50650; 37 antibodies from 15 providers.
DR DNASU; 252875; -.
DR Ensembl; ENSMUST00000040017; ENSMUSP00000039301; ENSMUSG00000042447.
DR GeneID; 252875; -.
DR KEGG; mmu:252875; -.
DR UCSC; uc009axk.2; mouse.
DR CTD; 54468; -.
DR MGI; MGI:2182066; Mios.
DR VEuPathDB; HostDB:ENSMUSG00000042447; -.
DR eggNOG; KOG1008; Eukaryota.
DR GeneTree; ENSGT00390000015038; -.
DR HOGENOM; CLU_005843_1_0_1; -.
DR InParanoid; Q8VE19; -.
DR OMA; QSHNSTC; -.
DR OrthoDB; 890767at2759; -.
DR PhylomeDB; Q8VE19; -.
DR TreeFam; TF324074; -.
DR Reactome; R-MMU-9639288; Amino acids regulate mTORC1.
DR BioGRID-ORCS; 252875; 13 hits in 72 CRISPR screens.
DR ChiTaRS; Mios; mouse.
DR PRO; PR:Q8VE19; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8VE19; protein.
DR Bgee; ENSMUSG00000042447; Expressed in rostral migratory stream and 242 other tissues.
DR Genevisible; Q8VE19; MM.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0061700; C:GATOR2 complex; ISO:MGI.
DR GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0034198; P:cellular response to amino acid starvation; ISO:MGI.
DR GO; GO:0032008; P:positive regulation of TOR signaling; ISO:MGI.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IBA:GO_Central.
DR GO; GO:0031503; P:protein-containing complex localization; ISO:MGI.
DR CDD; cd16691; mRING-H2-C3H3C2_Mio; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR037593; MIOS/Sea4.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR031488; Zn_ribbon_mio.
DR PANTHER; PTHR16453; PTHR16453; 1.
DR Pfam; PF17034; zinc_ribbon_16; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Lysosome; Membrane; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..875
FT /note="GATOR complex protein MIOS"
FT /id="PRO_0000329405"
FT REPEAT 58..100
FT /note="WD 1"
FT REPEAT 111..155
FT /note="WD 2"
FT REPEAT 182..221
FT /note="WD 3"
FT REPEAT 223..261
FT /note="WD 4"
FT REPEAT 265..306
FT /note="WD 5"
FT REPEAT 320..360
FT /note="WD 6"
FT REPEAT 395..437
FT /note="WD 7"
FT MOD_RES 759
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NXC5"
FT MOD_RES 766
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 341
FT /note="V -> S (in Ref. 2; AAH20002)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 875 AA; 98335 MW; 19F4F5C907F75214 CRC64;
MSGTKPDILW APHQVDRFVV CDSELSLYHV ESAVNSELKA GSLRLSEDSA ATLLSINSDT
PYMKCVAWYL NYDPECLLAV GQANGRVVLT SLGQDHNSKF KDLIGKEFVP KHARQCNTLA
WNPLDSNWLA AGLDKHRADF SVLIWDICSK YTPDIVPMEK VRLSAGEAET TLLVTKPLYE
LGQNDASLSL CWLPRDQKLL LAGMHRNLAI FDLRNTSQKM FVNTKAVQGV TVDPYFHDRV
ASFYEGQVAI WDLRKFEKPV LTLTEQPKPL TKVAWCPTRT GLLATLTRDS NIIRLYDMQH
TPTPIGDETE PTIIERSVQP CDNYIASFAW HPTSQNRMIV VTPNRTMSDF TVFERISLAW
SPITSLMWAC GRHLYECAEE ESDNSLEKDI ATKMRLRALS RYGLDTEQVW RNHILAGNED
PQLKSLWYTL HFMKQYTEDN DQKSPGNKGS LVYAGIKSIV KSSLGMVESS RHNWSGLDKQ
TDIQNLNEER ILALQLCGWI KKGTDVDVGP FLNSLVQEGE WERAAAVALF NLDIRRAIQI
LNEGASSEKG DLNLNVVAMA LSGYTDEKNS LWREMCSTLR LQLNNPYLCV MFAFLTSEAG
AYDGVLYENK VAVRDRVAFA CKFLGDAQLN KYIEKLTNEM KEAGNLEGIL LTGLTKDGVD
LMESYVDRTG DVQTASYCML QGSPLDVLKD ERVQYWIENY RNLLDAWRFW HKRAEFDIHR
SKLDPSSKPL AQVFVSCNFC GKSISYSCSS VPHQGRGFSQ YGVSGSPTKS KVTSCPGCRK
PLPRCALCLI NMGTPVSSCP GGSKSDEKVD LSKDKKLAQF NNWFTWCHNC RHGGHAGHML
SWFRDHAECP VSACTCKCMQ LDTTGNLVPA ETVQP
