ID   MIO_PONAB               Reviewed;         875 AA.
AC   Q5RCA2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=GATOR complex protein MIOS {ECO:0000305};
GN   Name=MIOS {ECO:0000250|UniProtKB:Q9NXC5};
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: As a component of the GATOR subcomplex GATOR2, functions
CC       within the amino acid-sensing branch of the TORC1 signaling pathway.
CC       Indirectly activates mTORC1 and the TORC1 signaling pathway through the
CC       inhibition of the GATOR1 subcomplex. It is negatively regulated by the
CC       upstream amino acid sensors SESN2 and CASTOR1.
CC       {ECO:0000250|UniProtKB:Q9NXC5}.
CC   -!- SUBUNIT: Within the GATOR complex, component of the GATOR2 subcomplex,
CC       made of MIOS, SEC13, SEH1L, WDR24 and WDR59. The GATOR complex strongly
CC       interacts with RRAGA/RRAGC and RRAGB/RRAGC heterodimers. The GATOR2
CC       complex interacts with CASTOR2 and CASTOR1; the interaction is
CC       negatively regulated by arginine. The GATOR2 complex interacts with the
CC       sestrins SESN1, SESN2 and SESN3; the interaction is negatively
CC       regulated by amino acids. {ECO:0000250|UniProtKB:Q9NXC5}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9NXC5}.
CC   -!- SIMILARITY: Belongs to the WD repeat mio family. {ECO:0000305}.
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DR   EMBL; CR858376; CAH90605.1; -; mRNA.
DR   RefSeq; NP_001128962.1; NM_001135490.1.
DR   AlphaFoldDB; Q5RCA2; -.
DR   SMR; Q5RCA2; -.
DR   STRING; 9601.ENSPPYP00000019940; -.
DR   GeneID; 100190802; -.
DR   KEGG; pon:100190802; -.
DR   CTD; 54468; -.
DR   eggNOG; KOG1008; Eukaryota.
DR   InParanoid; Q5RCA2; -.
DR   OrthoDB; 890767at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR   CDD; cd16691; mRING-H2-C3H3C2_Mio; 1.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR037593; MIOS/Sea4.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR031488; Zn_ribbon_mio.
DR   PANTHER; PTHR16453; PTHR16453; 1.
DR   Pfam; PF17034; zinc_ribbon_16; 1.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   2: Evidence at transcript level;
KW   Lysosome; Membrane; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..875
FT                   /note="GATOR complex protein MIOS"
FT                   /id="PRO_0000329406"
FT   REPEAT          61..100
FT                   /note="WD 1"
FT   REPEAT          111..155
FT                   /note="WD 2"
FT   REPEAT          182..221
FT                   /note="WD 3"
FT   REPEAT          223..261
FT                   /note="WD 4"
FT   REPEAT          265..306
FT                   /note="WD 5"
FT   REPEAT          395..437
FT                   /note="WD 6"
FT   MOD_RES         759
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NXC5"
FT   MOD_RES         766
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NXC5"
SQ   SEQUENCE   875 AA;  98401 MW;  FC83968BE26BA2DC CRC64;
     MSGTKPDILW APHQVDRFVV CDSELSLYHV ESTVNSELKA GSLRLSEDSA ATLLSINSDT
     PYMKCVACYL NYDPECLLAV GQANGRVVLT SLGQDHNSKF KDLIGKEFVP KHARQCNTLA
     WNPLDNNWLA AGLDKHRADF SVLIWDICSK YTPDIVPMEK VKLSAGETET TLLVTKPLYE
     LGQNDACLSL CWLPRDQKLL LAGMHRNLAI FDLRNTSQKM FVNTKAVQGV TVDPYFHDRV
     ASFYEGQVAI WDLRKFEKPV LTLTEQPKPL TKVAWCPTRT GLLATLTRDS NIIRLYDMQH
     TPTPIGDETE PTIIERSVQP CDNYIASFAW HPTSQNRMIV VTPNRTMSDF TVFERISLAW
     SPITSLMWAC GRHLYECTEE ENDNSLEKDI ATKMRLRALS RYGLDTEQVW RNHILAGNED
     PQLKSLWYTL HFMKQYTEDM DQKSPGNKGS LVYAGIKSIV KSSLGMVESS RHNWSGLDKQ
     SDIQNLNEER ILALQLCGWI KKGTDVDVGP FLNSLVQEGE RERAAAVALF NLDIRRAIQI
     LNEGASSEKG DLNLNVVAMA LSGYTGEKNS LWREMCSTLR LQLNNPYLCV MFAFLTSETG
     SYDGVLYENK VAVRDRVAFA CKFLSDTQLN RYIEKLTNEM KEAGNLEGIL LTGLTKDGVD
     LMESYVDRTG DVQTASYCML QGSPLDVLKD ERVQYWIENY RNLLDAWRFW HKRAEFDIHR
     SKLDPSSKPL AQVFVSCNFC GKSISYSCSA VPHQGRGFSQ YGVSGSPTKS KVTSCPGCRK
     PLPRCALCLI NTGTPVSSCP GGTKSDEKVD LSKDKKLAQF NNWFTWCHNC RHGGHAGHML
     SWFRDHAECP VSACTCKCMQ LDTTGNLVPA ETVQP