MIP3_ARATH
ID MIP3_ARATH Reviewed; 838 AA.
AC F4IP69; Q9SJI6;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Sec1 family domain-containing protein MIP3 {ECO:0000305};
DE AltName: Full=MAG2-interacting protein 3 {ECO:0000303|PubMed:24118572};
GN Name=MIP3 {ECO:0000303|PubMed:24118572};
GN OrderedLocusNames=At2g42700 {ECO:0000312|Araport:AT2G42700};
GN ORFNames=F14N22.4 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AEC10157.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH MAG2, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=24118572; DOI=10.1111/tpj.12347;
RA Li L., Shimada T., Takahashi H., Koumoto Y., Shirakawa M., Takagi J.,
RA Zhao X., Tu B., Jin H., Shen Z., Han B., Jia M., Kondo M., Nishimura M.,
RA Hara-Nishimura I.;
RT "MAG2 and three MAG2-INTERACTING PROTEINs form an ER-localized complex to
RT facilitate storage protein transport in Arabidopsis thaliana.";
RL Plant J. 76:781-791(2013).
CC -!- FUNCTION: Required for proper maturation of seed storage proteins.
CC Forms a complex with MAG2, ZW10/MIP1 and MIP2 on the endoplasmic
CC reticulum that may be responsible for efficient transport of seed
CC storage proteins. {ECO:0000269|PubMed:24118572}.
CC -!- SUBUNIT: Forms a complex with MAG2, ZW10/MIP1 and MIP2 on the
CC endoplasmic reticulum. {ECO:0000269|PubMed:24118572}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000305|PubMed:24118572}; Peripheral membrane protein
CC {ECO:0000305|PubMed:24118572}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=F4IP69-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Accumulation of the precursors of the two major
CC storage proteins albumin 2S and globulin 12S in dry seeds.
CC {ECO:0000269|PubMed:24118572}.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21736.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM15390.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006931; AAD21736.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC007087; AAM15390.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10157.1; -; Genomic_DNA.
DR PIR; B84857; B84857.
DR RefSeq; NP_181798.2; NM_129831.5. [F4IP69-1]
DR AlphaFoldDB; F4IP69; -.
DR BioGRID; 4207; 2.
DR STRING; 3702.AT2G42700.2; -.
DR PaxDb; F4IP69; -.
DR EnsemblPlants; AT2G42700.1; AT2G42700.1; AT2G42700. [F4IP69-1]
DR GeneID; 818870; -.
DR Gramene; AT2G42700.1; AT2G42700.1; AT2G42700. [F4IP69-1]
DR KEGG; ath:AT2G42700; -.
DR Araport; AT2G42700; -.
DR eggNOG; ENOG502QQIB; Eukaryota.
DR HOGENOM; CLU_017469_0_0_1; -.
DR OMA; CPFSPRV; -.
DR PRO; PR:F4IP69; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IP69; baseline and differential.
DR Genevisible; F4IP69; AT.
DR GO; GO:0033263; C:CORVET complex; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0051604; P:protein maturation; IMP:UniProtKB.
DR GO; GO:0006624; P:vacuolar protein processing; TAS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.1910; -; 1.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR SUPFAM; SSF56815; SSF56815; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..838
FT /note="Sec1 family domain-containing protein MIP3"
FT /id="PRO_0000430535"
FT REGION 637..677
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..659
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 660..676
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 838 AA; 91703 MW; F64B697560FFCF01 CRC64;
MALIDVAISC LNSIREIEED VKDAIVYIDA GCTESFQFVG AFPLFLELGA RAVCSLENMT
SLDAVADWNS KSDCAKRIVI MTSRLLNDAH RYMLRCLSTH EGVQRCTVFT SISEGSHSAI
PDSPLGPDAY REYETLLVQD YNEHTKKSDK ISKDKGVSKF SSALESLTME PIESENVDIS
SGGAQGLVVS VHHFPLIICP FTPRAFVLPS QGSVAEASLS RQHEDSLSFG LPPISTGSMS
DTDDVPPGAT LTAHFLYQLA LKMELKLEIF SLGDQSKNVG KILTDMSSVY DVARRKRSAG
LLLVDRTLDL ITPCCHGDSL FDRIFSSLPR AERFSSQAQL KQGVPSINRP SLDVQVPLGE
LLNEEPSKIR DSGLPEGIEA FLRGWDSYTS APQNVGLFNE CDKKSTTNWT ELLNGSLVAT
ECFRGTPYLE AMIDRKTKDG SVLVKKWLQE ALRRENISVN VRARPGYATK PELQAMIKAL
SQSQSSLLKN KGIIQLGAAT AAALDESQSA KWDTFSSAEM MLNVSAGDTS QGLAAQISDL
INKSAVAELQ AKKNEKPDSS SRGLLSFRDA LLLTIVGYIL AGENFPTSGS GGPFSWQEEH
FLKEAIVDAV LENPSAGNLK FLNGLTEELE GRLNRLKSEE TKEIPSDDQL DIDALDDDPW
GKWGDEEEEE VDNSKADESY DDMQLKLDLR DRVDSLFRFL HKLSSLRTRN LPLREGSLAS
ESSFPGEPSG NKGLVYRLIT KVLSKQEIPG LEYHSSTVGR FIKSGFGRFG LGQAKPSLAD
QSVILVFVIG GINGIEVLEA QEAVSESGRP DINLVIGGTT LLTPDDMFEL LLGQFSHF
