MIP6_YEAST
ID MIP6_YEAST Reviewed; 659 AA.
AC P38760; D3DKW0;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=RNA-binding protein MIP6;
DE AltName: Full=MEX67-interacting protein 6;
GN Name=MIP6; OrderedLocusNames=YHR015W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INTERACTION WITH MEX67.
RX PubMed=9214641; DOI=10.1093/emboj/16.11.3256;
RA Segref A., Sharma K., Doye V., Hellwig A., Huber J., Luehrmann R., Hurt E.;
RT "Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA
RT and nuclear pores.";
RL EMBO J. 16:3256-3271(1997).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- SUBUNIT: Interacts with MEX67. {ECO:0000269|PubMed:9214641}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 98 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U10400; AAB68942.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06704.1; -; Genomic_DNA.
DR PIR; S46788; S46788.
DR RefSeq; NP_011879.1; NM_001179145.1.
DR PDB; 5D77; X-ray; 1.30 A; A=313-388.
DR PDB; 5D78; X-ray; 1.25 A; A=313-387.
DR PDBsum; 5D77; -.
DR PDBsum; 5D78; -.
DR AlphaFoldDB; P38760; -.
DR SMR; P38760; -.
DR BioGRID; 36444; 31.
DR DIP; DIP-6647N; -.
DR IntAct; P38760; 2.
DR STRING; 4932.YHR015W; -.
DR PaxDb; P38760; -.
DR PRIDE; P38760; -.
DR EnsemblFungi; YHR015W_mRNA; YHR015W; YHR015W.
DR GeneID; 856408; -.
DR KEGG; sce:YHR015W; -.
DR SGD; S000001057; MIP6.
DR VEuPathDB; FungiDB:YHR015W; -.
DR eggNOG; KOG0123; Eukaryota.
DR GeneTree; ENSGT00940000176742; -.
DR HOGENOM; CLU_020939_0_0_1; -.
DR InParanoid; P38760; -.
DR OMA; IICGLHF; -.
DR BioCyc; YEAST:G3O-31077-MON; -.
DR Reactome; R-SCE-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-SCE-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-SCE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:P38760; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38760; protein.
DR GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005628; C:prospore membrane; IDA:SGD.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0008143; F:poly(A) binding; IBA:GO_Central.
DR GO; GO:0008266; F:poly(U) RNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0010609; P:mRNA localization resulting in post-transcriptional regulation of gene expression; IGI:SGD.
DR GO; GO:0016071; P:mRNA metabolic process; IGI:SGD.
DR GO; GO:0006417; P:regulation of translation; IGI:SGD.
DR Gene3D; 3.30.70.330; -; 4.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 4.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..659
FT /note="RNA-binding protein MIP6"
FT /id="PRO_0000082032"
FT DOMAIN 111..189
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 199..267
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 313..389
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 314..318
FT /evidence="ECO:0007829|PDB:5D78"
FT HELIX 326..333
FT /evidence="ECO:0007829|PDB:5D78"
FT TURN 334..336
FT /evidence="ECO:0007829|PDB:5D78"
FT STRAND 339..344
FT /evidence="ECO:0007829|PDB:5D78"
FT STRAND 354..361
FT /evidence="ECO:0007829|PDB:5D78"
FT HELIX 362..372
FT /evidence="ECO:0007829|PDB:5D78"
FT STRAND 375..377
FT /evidence="ECO:0007829|PDB:5D78"
FT STRAND 380..386
FT /evidence="ECO:0007829|PDB:5D78"
SQ SEQUENCE 659 AA; 75920 MW; DF8E3F4D12A63871 CRC64;
MPNSHGNVLN NISLNSKQNP RSISKSCPND KDARQKSFKT ISAQALVRVQ GAGYKLGDVK
LKDAEVKEKN SLKKYDCKNA TQEKKEQEQV FEKTVAKGSV QKYITKTSKT NSLFIGNLKS
TVTEEMLRKI FKRYQSFESA KVCRDFLTKK SLGYGYLNFK DKNDAESARK EFNYTVFFGQ
EVKIMPSMKN TLFRKNIGTN VFFSNLPLEN PQLTTRSFYL IMIEYGNVLS CLLERRKNIG
FVYFDNDISA RNVIKKYNNQ EFFGNKIICG LHFDKEVRTR PEFTKRKKMI GSDIVIEDEL
LASNNLSDNA RSKTILVKNL PSDTTQEEVL DYFSTIGPIK SVFISEKQAN TPHKAFVTYK
NEEESKKAQK CLNKTIFKNH TIWVGPGKDK PVHNQIGTNK KTKVYLKNLS FNCNKEFISQ
LCLQEKIRFS EIKITNYNSL NWTFCGHVEC FSRSDAERLF NILDRRLIGS SLVEASWSKN
NDNILNEIDY DDGNNNENYK KLINISSMMR FRTQELSAHQ KGLTSQFQQV VSPFSSYSNS
YTNMNSLVAT PMKPHPAFNL ITNTVDEKLH QPKRTKQENA EILESLKKII NRNLQRISIS
GLNKEENLRS ISEFIFDVFW EHDSERLSHF LLMTNTSLES QKILQKQVTR AAESLGFTV
