MIR1_GIBZE
ID MIR1_GIBZE Reviewed; 609 AA.
AC I1RAK8;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=MFS siderochrome iron transporter 1 {ECO:0000303|PubMed:20507510};
GN Name=MIR1 {ECO:0000303|PubMed:20507510};
GN ORFNames=FG00539, FGRAMPH1_01T01375;
OS Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=229533;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=17823352; DOI=10.1126/science.1143708;
RA Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT "The Fusarium graminearum genome reveals a link between localized
RT polymorphism and pathogen specialization.";
RL Science 317:1400-1402(2007).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA Hammond-Kosack K.E.;
RT "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT graminearum.";
RL BMC Genomics 16:544-544(2015).
RN [4]
RP FUNCTION.
RX PubMed=17056706; DOI=10.1105/tpc.106.045633;
RA Oide S., Moeder W., Krasnoff S., Gibson D., Haas H., Yoshioka K.,
RA Turgeon B.G.;
RT "NPS6, encoding a nonribosomal peptide synthetase involved in siderophore-
RT mediated iron metabolism, is a conserved virulence determinant of plant
RT pathogenic ascomycetes.";
RL Plant Cell 18:2836-2853(2006).
RN [5]
RP FUNCTION.
RX PubMed=17043871; DOI=10.1007/s00294-006-0103-0;
RA Tobiasen C., Aahman J., Ravnholt K.S., Bjerrum M.J., Grell M.N., Giese H.;
RT "Nonribosomal peptide synthetase (NPS) genes in Fusarium graminearum, F.
RT culmorum and F. pseudograminearium and identification of NPS2 as the
RT producer of ferricrocin.";
RL Curr. Genet. 51:43-58(2007).
RN [6]
RP FUNCTION.
RX PubMed=17601875; DOI=10.1128/ec.00111-07;
RA Oide S., Krasnoff S.B., Gibson D.M., Turgeon B.G.;
RT "Intracellular siderophores are essential for ascomycete sexual development
RT in heterothallic Cochliobolus heterostrophus and homothallic Gibberella
RT zeae.";
RL Eukaryot. Cell 6:1339-1353(2007).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=20507510; DOI=10.1111/j.1364-3703.2007.00401.x;
RA Greenshields D.L., Liu G., Feng J., Selvaraj G., Wei Y.;
RT "The siderophore biosynthetic gene SID1, but not the ferroxidase gene FET3,
RT is required for full Fusarium graminearum virulence.";
RL Mol. Plant Pathol. 8:411-421(2007).
CC -!- FUNCTION: Major facilitator transporter involved in extracellular
CC siderophore uptake (PubMed:20507510). Gibberella zeae produces
CC extracellular coprogen-type siderophores as well as the intracellular
CC siderophore ferricrocin (PubMed:17056706). The role of extracellular
CC siderophores is to supply iron to the fungus during plant infection,
CC and the intracellular ferricrocin is required for intracellular iron
CC distribution and storage with a crucial role in ascus and ascospore
CC development (PubMed:17056706, PubMed:17043871, PubMed:17601875).
CC {ECO:0000269|PubMed:17043871, ECO:0000269|PubMed:17056706,
CC ECO:0000269|PubMed:17601875, ECO:0000269|PubMed:20507510}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Not expressed during infection (PubMed:20507510).
CC {ECO:0000269|PubMed:20507510}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; HG970332; CEF72488.1; -; Genomic_DNA.
DR RefSeq; XP_011316218.1; XM_011317916.1.
DR AlphaFoldDB; I1RAK8; -.
DR STRING; 5518.FGSG_00539P0; -.
DR GeneID; 23548027; -.
DR KEGG; fgr:FGSG_00539; -.
DR VEuPathDB; FungiDB:FGRAMPH1_01G01375; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_012970_1_0_1; -.
DR InParanoid; I1RAK8; -.
DR Proteomes; UP000070720; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR SUPFAM; SSF103473; SSF103473; 2.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..609
FT /note="MFS siderochrome iron transporter 1"
FT /id="PRO_0000444439"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 469..489
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 573..593
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..52
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 609 AA; 66264 MW; 60F4B8F06CCF9FC6 CRC64;
MSALTKIREG LAREDNTHVA GTLAPHEKEI HETPEVVSEV GPDHDKEATA GAINPDDKGN
DSDVPSEDVQ NGVKEIQAIT LTWGKGSLAA LLCLIWTLFL ISGFRGSFYL VLVPYVTSEW
SAHSLMTTIP IVSDAMTAAC YIPMAKALDV WGRAEGFLLM SGFATLGLIL MAVSQNLATF
CAAQVFYSVG WGGMIYAVGV LAADASNLRN RGLAFAFTSS PYMITAFAGS KAAAAFVIDV
KNWRWGFGWI ALVLPCVTIP LFLVLKVNLR KAFKNGTVTK TTRNRGFFGS IWWAFNEFDV
IGIFLFGGGL VVFLLPFNLA GHAPNGWSTG YIIAMIIVGF CTLIFFGVWE YWLAPVPFLQ
GRFLLDRSVV AACMIDLTYQ VSYYTWNYFF TSFLQVVVNL GPAEAGYVNS TFQVVSGVLL
FIVGFLIRKT GFYKWTFYFA VPIYIFALGL MIHFRAPNQY VGYIIMCEIF ISIGGAVFIL
VMQLAVLAAV AHQYVAAALA TLYVAGGVGG AVGGAISGAI WTNSFIPQLI KRLPESEVAN
ATLIAGSIVN QLAYPVDSPA RLAIQESYGF AQIRMLAAGV GIASLFFIWV PMLRNIDVKK
LKQTKGLVL
