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MIRO2_BOVIN
ID   MIRO2_BOVIN             Reviewed;         618 AA.
AC   Q5E9M9; A6H7G4; Q7YSE6;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Mitochondrial Rho GTPase 2;
DE            Short=MIRO-2;
DE            EC=3.6.5.-;
DE   AltName: Full=Ras homolog gene family member T2;
GN   Name=RHOT2; Synonyms=ARHT2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15218247; DOI=10.1159/000078568;
RA   Shan Y., Hexige S., Guo Z., Wan B., Chen K., Chen X., Ma L., Huang C.,
RA   Zhao S., Yu L.;
RT   "Cloning and characterization of the mouse Arht2 gene which encodes a
RT   putative atypical GTPase.";
RL   Cytogenet. Genome Res. 106:91-97(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial GTPase involved in mitochondrial trafficking.
CC       Probably involved in control of anterograde transport of mitochondria
CC       and their subcellular distribution (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the kinesin-binding proteins TRAK1/OIP106 and
CC       TRAK2/GRIF1, forming a link between mitochondria and the trafficking
CC       apparatus of the microtubules (By similarity). Interacts with ARMCX3
CC       (By similarity). Found in a complex with KIF5B, OGT, RHOT1 and TRAK1
CC       (By similarity). {ECO:0000250|UniProtKB:Q8IXI1,
CC       ECO:0000250|UniProtKB:Q8JZN7}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC       Single-pass type IV membrane protein {ECO:0000250}. Note=Colocalizes
CC       with MGARP and RHOT2 at the mitochondria. {ECO:0000250}.
CC   -!- PTM: Ubiquitinated by PRKN in a PINK1-dependent manner, leading to its
CC       degradation. {ECO:0000250|UniProtKB:Q8IXI1}.
CC   -!- SIMILARITY: Belongs to the mitochondrial Rho GTPase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00757, ECO:0000305}.
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DR   EMBL; AY247740; AAP04409.2; -; mRNA.
DR   EMBL; BT020891; AAX08908.1; -; mRNA.
DR   EMBL; BC146236; AAI46237.1; -; mRNA.
DR   RefSeq; NP_847886.3; NM_178316.3.
DR   AlphaFoldDB; Q5E9M9; -.
DR   SMR; Q5E9M9; -.
DR   STRING; 9913.ENSBTAP00000005979; -.
DR   PaxDb; Q5E9M9; -.
DR   PRIDE; Q5E9M9; -.
DR   Ensembl; ENSBTAT00000005979; ENSBTAP00000005979; ENSBTAG00000004551.
DR   GeneID; 352959; -.
DR   KEGG; bta:352959; -.
DR   CTD; 89941; -.
DR   VEuPathDB; HostDB:ENSBTAG00000004551; -.
DR   VGNC; VGNC:33954; RHOT2.
DR   eggNOG; KOG1707; Eukaryota.
DR   GeneTree; ENSGT00940000158109; -.
DR   InParanoid; Q5E9M9; -.
DR   OMA; ELHATSF; -.
DR   OrthoDB; 538388at2759; -.
DR   Proteomes; UP000009136; Chromosome 25.
DR   Bgee; ENSBTAG00000004551; Expressed in retina and 104 other tissues.
DR   GO; GO:0031307; C:integral component of mitochondrial outer membrane; ISS:UniProtKB.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0019725; P:cellular homeostasis; ISS:UniProtKB.
DR   GO; GO:0097345; P:mitochondrial outer membrane permeabilization; ISS:UniProtKB.
DR   GO; GO:0007005; P:mitochondrion organization; IBA:GO_Central.
DR   GO; GO:0047497; P:mitochondrion transport along microtubule; ISS:UniProtKB.
DR   GO; GO:0010821; P:regulation of mitochondrion organization; IBA:GO_Central.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR013566; EF_hand_assoc_1.
DR   InterPro; IPR013567; EF_hand_assoc_2.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR021181; Miro.
DR   InterPro; IPR020860; MIRO_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001806; Small_GTPase.
DR   InterPro; IPR003578; Small_GTPase_Rho.
DR   PANTHER; PTHR24072; PTHR24072; 1.
DR   Pfam; PF08355; EF_assoc_1; 1.
DR   Pfam; PF08356; EF_assoc_2; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PIRSF; PIRSF037488; Mt_Rho_GTPase; 1.
DR   SMART; SM00054; EFh; 2.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
DR   PROSITE; PS51423; MIRO; 2.
PE   2: Evidence at transcript level;
KW   Calcium; GTP-binding; Hydrolase; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion outer membrane; Nucleotide-binding; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Ubl conjugation.
FT   CHAIN           1..618
FT                   /note="Mitochondrial Rho GTPase 2"
FT                   /id="PRO_0000239317"
FT   TOPO_DOM        1..592
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        593..615
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        616..618
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          2..168
FT                   /note="Miro 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00757"
FT   DOMAIN          184..219
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          304..339
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          415..576
FT                   /note="Miro 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00757"
FT   BINDING         11..18
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         57..61
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         118..121
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         197
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         199
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         201
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         208
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         317
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         319
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         321
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         328
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         424..431
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         459..463
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         524..527
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        245
FT                   /note="Q -> P (in Ref. 1; AAP04409)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        249
FT                   /note="D -> E (in Ref. 1; AAP04409)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   618 AA;  68152 MW;  1CEA30B829B407E7 CRC64;
     MKRDVRILLL GEAQVGKTSL ILSLVGEEFP EEVPARAEEI TIPADVTPEK VPTHIVDYSE
     TEQTVEELQG EIDKADVVCV VYDVSEEATV EKIRTKWIPL VNGDTKRGPR VPIILVGNKS
     DLRPGGSMEA VLPIMSQFPE IETCVECSAK NLKNISELFY YAQKAVLHPT APLYDPEAKQ
     LRPACAQALT RIFRLSDQDM DQALSDQELN AFQTSCFGHP LAPQALEDVK MVVSKNVVGG
     VRDDQLTLDG FLFLNTLFIQ RGRHETTWTI LRRFGYGDSL ELTADYLCPP LRVPPGCSAE
     LNHRGYQFVQ RMFEKHDQDR DGALSPAELQ SLFSVFPAAP WGPHLPSTVR TKAGRLPLHG
     YLCQWTLVTY LDVRRSLEHL GYLGYPTLCE QDSQAHAITV TREKRLDQEK GQTQRNVLLC
     KVVGARGVGK SSFLRAFLGH SLGHQDAGEP SVYAIDTVQV NGQEKYLILC EVAADSLLTA
     SADASCDVAC LMFDGSDLRS FALCASVYKQ HYMDGQTPCL FVCSKADLPG GVPLPGLSPA
     EFCRRHRLPT PTLFSCAGPV EPCMGIFTRL ATMATFPHLV HGQLQATSFW LRVALGAMGA
     AVAAILSFSL YRVLVKSR
 
 
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