ID   MIS12_ARATH             Reviewed;         238 AA.
AC   Q2V0Z5; Q9FJA7;
DT   13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Protein MIS12 homolog {ECO:0000305};
DE   AltName: Full=Protein MINICHROMOSOME INSTABILITY 12;
DE            Short=AtMIS12;
DE            Short=Protein MIS12-LIKE;
GN   Name=MIS12 {ECO:0000303|PubMed:16331414};
GN   OrderedLocusNames=At5g35520 {ECO:0000312|Araport:AT5G35520};
GN   ORFNames=MOK9.12 {ECO:0000312|EMBL:BAB08711.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Columbia;
RX   PubMed=16331414; DOI=10.1007/s10577-005-1016-3;
RA   Sato H., Shibata F., Murata M.;
RT   "Characterization of a Mis12 homologue in Arabidopsis thaliana.";
RL   Chromosome Res. 13:827-834(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA   Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT   features of the regions of 1,013,767 bp covered by sixteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:297-308(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=21695238; DOI=10.1371/journal.pgen.1002121;
RA   Ravi M., Shibata F., Ramahi J.S., Nagaki K., Chen C., Murata M., Chan S.W.;
RT   "Meiosis-specific loading of the centromere-specific histone CENH3 in
RT   Arabidopsis thaliana.";
RL   PLoS Genet. 7:E1002121-E1002121(2011).
CC   -!- FUNCTION: Constitutive component of kinetochores that is essential for
CC       proper cell division during mitotic cell cycle (Probable). May play a
CC       role in the modulation of centromere during meiosis (PubMed:21695238).
CC       {ECO:0000269|PubMed:21695238, ECO:0000305|PubMed:16331414}.
CC   -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC       {ECO:0000269|PubMed:16331414, ECO:0000269|PubMed:21695238}.
CC       Note=Localizes at centromeric regions throughout the mitotic cell
CC       cycle. Co-localizes with CENH3 during the cell cycle, from interphase
CC       to anaphase (PubMed:16331414). Localized at centromeres in meiotic
CC       cells (PubMed:21695238). {ECO:0000269|PubMed:16331414,
CC       ECO:0000269|PubMed:21695238}.
CC   -!- DISRUPTION PHENOTYPE: Embryonic lethality when homozygous.
CC       {ECO:0000269|PubMed:16331414}.
CC   -!- SIMILARITY: Belongs to the mis12 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08711.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB205161; BAE66672.1; -; Genomic_DNA.
DR   EMBL; AB015477; BAB08711.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED93975.1; -; Genomic_DNA.
DR   RefSeq; NP_198402.2; NM_122943.3.
DR   AlphaFoldDB; Q2V0Z5; -.
DR   SMR; Q2V0Z5; -.
DR   STRING; 3702.AT5G35520.1; -.
DR   PaxDb; Q2V0Z5; -.
DR   PRIDE; Q2V0Z5; -.
DR   ProteomicsDB; 250708; -.
DR   EnsemblPlants; AT5G35520.1; AT5G35520.1; AT5G35520.
DR   GeneID; 833516; -.
DR   Gramene; AT5G35520.1; AT5G35520.1; AT5G35520.
DR   KEGG; ath:AT5G35520; -.
DR   Araport; AT5G35520; -.
DR   TAIR; locus:2169508; AT5G35520.
DR   eggNOG; ENOG502QVA6; Eukaryota.
DR   HOGENOM; CLU_101939_0_0_1; -.
DR   InParanoid; Q2V0Z5; -.
DR   OMA; HEMFQEM; -.
DR   OrthoDB; 1482964at2759; -.
DR   PRO; PR:Q2V0Z5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q2V0Z5; baseline and differential.
DR   GO; GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
DR   GO; GO:0000818; C:nuclear MIS12/MIND complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051382; P:kinetochore assembly; IBA:GO_Central.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR   GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR   InterPro; IPR008685; Centromere_Mis12.
DR   PANTHER; PTHR14527; PTHR14527; 1.
DR   Pfam; PF05859; Mis12; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Reference proteome.
FT   CHAIN           1..238
FT                   /note="Protein MIS12 homolog"
FT                   /id="PRO_0000435852"
FT   COILED          117..149
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   238 AA;  27126 MW;  4171A12A1850AA3B CRC64;
     MEGSKSEAVF DSMNLNPQIF INEAINSVED YVDQAFDFYA RDASKSLKIK GSDKQKSQAL
     SNGIARVRGL LLSVIDNRLK LWESYSLRFC FAVPDGFVLP KSEESSSVHQ DGLYDLELDA
     ELDSLRDKLN VVGKRSVELD SELQALERSS VSRERSLRLV NEALELYDES SMDEIFKEMT
     KMASELRASV ERLKTRRMKA SESAKVKRLK NHGKEFSAMT FDGKLEDLEK FQAELRKM