MIS12_MOUSE
ID MIS12_MOUSE Reviewed; 206 AA.
AC Q9CY25; Q3V261; Q5QNU7; Q5QNU8; Q5QNU9;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein MIS12 homolog;
GN Name=Mis12 {ECO:0000312|MGI:MGI:1914389};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:BAB27314.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAB27314.1}, and
RC NOD {ECO:0000312|EMBL:BAE32656.1};
RC TISSUE=Dendritic cell {ECO:0000312|EMBL:BAE32656.1},
RC Egg {ECO:0000312|EMBL:BAE24195.1}, Embryo {ECO:0000312|EMBL:BAE20937.1},
RC and Embryonic liver {ECO:0000312|EMBL:BAB27314.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000312|EMBL:AAH26790.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland {ECO:0000312|EMBL:AAH26790.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Part of the MIS12 complex which is required for normal
CC chromosome alignment and segregation and for kinetochore formation
CC during mitosis. Essential for proper kinetochore microtubule
CC attachments. {ECO:0000250|UniProtKB:Q9H081}.
CC -!- SUBUNIT: Component of the MIS12 complex composed of MIS12, DSN1, NSL1
CC and PMF1. Also interacts with KNL1, CBX3, CBX5, NDC80 and ZWINT.
CC {ECO:0000250|UniProtKB:Q9H081}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q9H081}. Note=Associated with the kinetochore.
CC {ECO:0000250|UniProtKB:Q9H081}.
CC -!- SIMILARITY: Belongs to the mis12 family. {ECO:0000305}.
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DR EMBL; AK010995; BAB27314.1; -; mRNA.
DR EMBL; AK132010; BAE20937.1; -; mRNA.
DR EMBL; AK139957; BAE24195.1; -; mRNA.
DR EMBL; AK154531; BAE32656.1; -; mRNA.
DR EMBL; AL596136; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC026790; AAH26790.1; -; mRNA.
DR CCDS; CCDS24973.1; -.
DR RefSeq; NP_080269.1; NM_025993.2.
DR RefSeq; XP_006534031.1; XM_006533968.2.
DR RefSeq; XP_006534032.1; XM_006533969.1.
DR AlphaFoldDB; Q9CY25; -.
DR SMR; Q9CY25; -.
DR BioGRID; 211970; 84.
DR ComplexPortal; CPX-5701; Kinetochore MIS12 complex.
DR IntAct; Q9CY25; 87.
DR MINT; Q9CY25; -.
DR STRING; 10090.ENSMUSP00000039500; -.
DR iPTMnet; Q9CY25; -.
DR PhosphoSitePlus; Q9CY25; -.
DR EPD; Q9CY25; -.
DR MaxQB; Q9CY25; -.
DR PaxDb; Q9CY25; -.
DR PeptideAtlas; Q9CY25; -.
DR PRIDE; Q9CY25; -.
DR ProteomicsDB; 295617; -.
DR Antibodypedia; 23713; 172 antibodies from 24 providers.
DR Ensembl; ENSMUST00000048807; ENSMUSP00000039500; ENSMUSG00000040599.
DR Ensembl; ENSMUST00000164220; ENSMUSP00000127782; ENSMUSG00000040599.
DR GeneID; 67139; -.
DR KEGG; mmu:67139; -.
DR UCSC; uc007jxm.1; mouse.
DR CTD; 79003; -.
DR MGI; MGI:1914389; Mis12.
DR VEuPathDB; HostDB:ENSMUSG00000040599; -.
DR eggNOG; ENOG502RXZ1; Eukaryota.
DR GeneTree; ENSGT00390000018665; -.
DR HOGENOM; CLU_097032_0_0_1; -.
DR InParanoid; Q9CY25; -.
DR OMA; DYLFEMM; -.
DR OrthoDB; 1197218at2759; -.
DR PhylomeDB; Q9CY25; -.
DR TreeFam; TF101136; -.
DR Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR Reactome; R-MMU-68877; Mitotic Prometaphase.
DR Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR BioGRID-ORCS; 67139; 22 hits in 73 CRISPR screens.
DR ChiTaRS; Mis12; mouse.
DR PRO; PR:Q9CY25; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9CY25; protein.
DR Bgee; ENSMUSG00000040599; Expressed in ear vesicle and 244 other tissues.
DR ExpressionAtlas; Q9CY25; baseline and differential.
DR Genevisible; Q9CY25; MM.
DR GO; GO:0000775; C:chromosome, centromeric region; ISO:MGI.
DR GO; GO:0000776; C:kinetochore; ISO:MGI.
DR GO; GO:0000444; C:MIS12/MIND type complex; ISS:UniProtKB.
DR GO; GO:0031617; C:NMS complex; IC:ComplexPortal.
DR GO; GO:0000818; C:nuclear MIS12/MIND complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR GO; GO:0051382; P:kinetochore assembly; ISS:UniProtKB.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR InterPro; IPR008685; Centromere_Mis12.
DR PANTHER; PTHR14527; PTHR14527; 1.
DR Pfam; PF05859; Mis12; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Kinetochore; Mitosis; Reference proteome.
FT CHAIN 1..206
FT /note="Protein MIS12 homolog"
FT /id="PRO_0000248235"
FT COILED 102..206
FT /evidence="ECO:0000255"
FT CONFLICT 161
FT /note="D -> N (in Ref. 1; BAE20937)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 206 AA; 24131 MW; 3492940A9EC23254 CRC64;
MSVDPMAYEA QFFGFTPQTC LLRIYVAFQD HLFEVMQAVE QVILKKLEDI PNCEITPVQT
RKCTEKFLCF MKGRFDNLFG KMEQLILQSI LCIPPNILLP EDKCQETNPF SEEKLELLQQ
EIKELQEKYK VELCTEQALL AELEEQKTVK AKLRETLTFF DELENIGRYQ GTSNFRESLA
SLVQSCRKLQ SIRDNVEKES RRLETQ
