MIS12_SCHPO
ID MIS12_SCHPO Reviewed; 259 AA.
AC Q9Y738;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Centromere protein mis12;
DE AltName: Full=NMS complex subunit mis12;
GN Name=mis12; ORFNames=SPBC409.04c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=972 / ATCC 24843;
RX PubMed=10398680; DOI=10.1101/gad.13.13.1664;
RA Goshima G., Saitoh S., Yanagida M.;
RT "Proper metaphase spindle length is determined by centromere proteins Mis12
RT and Mis6 required for faithful chromosome segregation.";
RL Genes Dev. 13:1664-1677(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND PHOSPHORYLATION AT SER-190;
RP THR-192 AND SER-213.
RX PubMed=12773390; DOI=10.1093/emboj/cdg266;
RA Goshima G., Iwasaki O., Obuse C., Yanagida M.;
RT "The role of Ppe1/PP6 phosphatase for equal chromosome segregation in
RT fission yeast kinetochore.";
RL EMBO J. 22:2752-2763(2003).
RN [4]
RP INTERACTION WITH MAL2.
RX PubMed=12242294; DOI=10.1128/mcb.22.20.7168-7183.2002;
RA Jin Q.-W., Pidoux A.L., Decker C., Allshire R.C., Fleig U.;
RT "The mal2p protein is an essential component of the fission yeast
RT centromere.";
RL Mol. Cell. Biol. 22:7168-7183(2002).
RN [5]
RP INTERACTION WITH MIS14.
RX PubMed=15369671; DOI=10.1016/j.cell.2004.09.002;
RA Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M.;
RT "Mis16 and Mis18 are required for CENP-A loading and histone deacetylation
RT at centromeres.";
RL Cell 118:715-729(2004).
RN [6]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [7]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT "Reconstruction of the kinetochore during meiosis in fission yeast
RT Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 17:5173-5184(2006).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super
CC complex which has a role in kinetochore function during late meiotic
CC prophase and throughout the mitotic cell cycle. Required for correct
CC segregation of chromosomes and for maintaining the inner centromere
CC structure. {ECO:0000269|PubMed:12773390}.
CC -!- SUBUNIT: Component of the NMS super complex which consists of mis12,
CC mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7, spc24 and spc25. Interacts
CC with dis1, mal2, mis14, ppe1 and ekc1. {ECO:0000269|PubMed:12242294,
CC ECO:0000269|PubMed:12773390, ECO:0000269|PubMed:15369671,
CC ECO:0000269|PubMed:16079914, ECO:0000269|PubMed:17035632}.
CC -!- INTERACTION:
CC Q9Y738; Q9Y812: cnp1; NbExp=3; IntAct=EBI-1002822, EBI-1153281;
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:12773390}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:12773390}.
CC -!- SIMILARITY: Belongs to the mis12 family. {ECO:0000305}.
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DR EMBL; AB027472; BAA77791.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB52606.1; -; Genomic_DNA.
DR PIR; T40432; T40432.
DR RefSeq; NP_595454.1; NM_001021364.2.
DR PDB; 5WWL; X-ray; 2.40 A; M=1-215.
DR PDBsum; 5WWL; -.
DR AlphaFoldDB; Q9Y738; -.
DR SMR; Q9Y738; -.
DR BioGRID; 277022; 42.
DR IntAct; Q9Y738; 3.
DR STRING; 4896.SPBC409.04c.1; -.
DR iPTMnet; Q9Y738; -.
DR MaxQB; Q9Y738; -.
DR PaxDb; Q9Y738; -.
DR EnsemblFungi; SPBC409.04c.1; SPBC409.04c.1:pep; SPBC409.04c.
DR GeneID; 2540494; -.
DR KEGG; spo:SPBC409.04c; -.
DR PomBase; SPBC409.04c; mis12.
DR VEuPathDB; FungiDB:SPBC409.04c; -.
DR eggNOG; ENOG502S72R; Eukaryota.
DR HOGENOM; CLU_046437_0_0_1; -.
DR InParanoid; Q9Y738; -.
DR OMA; EGLHKFE; -.
DR PhylomeDB; Q9Y738; -.
DR PRO; PR:Q9Y738; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0034506; C:chromosome, centromeric core domain; IDA:PomBase.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0000939; C:inner kinetochore; IDA:PomBase.
DR GO; GO:0031617; C:NMS complex; IDA:PomBase.
DR GO; GO:0000818; C:nuclear MIS12/MIND complex; IDA:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051382; P:kinetochore assembly; IBA:GO_Central.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR GO; GO:0034501; P:protein localization to kinetochore; IBA:GO_Central.
DR InterPro; IPR008685; Centromere_Mis12.
DR PANTHER; PTHR14527; PTHR14527; 1.
DR Pfam; PF05859; Mis12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Centromere; Chromosome;
KW Coiled coil; Kinetochore; Meiosis; Mitosis; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..259
FT /note="Centromere protein mis12"
FT /id="PRO_0000096492"
FT COILED 123..156
FT /evidence="ECO:0000255"
FT MOD_RES 190
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:12773390"
FT MOD_RES 192
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:12773390"
FT MOD_RES 213
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:12773390"
FT HELIX 3..7
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 9..36
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 41..44
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 46..77
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 84..89
FT /evidence="ECO:0007829|PDB:5WWL"
FT TURN 95..98
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 103..155
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 166..187
FT /evidence="ECO:0007829|PDB:5WWL"
FT HELIX 196..212
FT /evidence="ECO:0007829|PDB:5WWL"
SQ SEQUENCE 259 AA; 30195 MW; B4D0B64BB9EB9007 CRC64;
MLVELLEFTP LSFIDDVINI TNQLLYKGVN GVDKAFSQTR FAKKAPQEIE EGLHKFEVLF
ESVVDRYYDG FEVYTLRNIF SYPPELKGYM RTFGKDVDYS ITTEQDAAMD QAIQEAAEKL
VVKMQLRRDL RMRLSRKREK KTEIEKHLER ISFLNKVPEN WQVTLPETTD FLLDQLGNLQ
HAVKRVVEAS PTVHSREVDE RITYLEKGYE RLSNPIDQQK DFWSHHLSKL ESTANTETAN
NIHKLLLSSE KDVGHTDEP
