ID   MIS13_SCHPO             Reviewed;         329 AA.
AC   Q9UUB5;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Kinetochore protein mis13;
DE   AltName: Full=NMS complex subunit mis13;
GN   Name=mis13; Synonyms=cnl1, cnl3; ORFNames=SPBC409.09c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION IN THE NMS COMPLEX.
RX   PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA   Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT   "Molecular analysis of kinetochore architecture in fission yeast.";
RL   EMBO J. 24:2919-2930(2005).
RN   [3]
RP   IDENTIFICATION IN THE NMS COMPLEX.
RX   PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA   Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT   "Reconstruction of the kinetochore during meiosis in fission yeast
RT   Schizosaccharomyces pombe.";
RL   Mol. Biol. Cell 17:5173-5184(2006).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super
CC       complex which has a role in kinetochore function during late meiotic
CC       prophase and throughout the mitotic cell cycle. Required for correct
CC       segregation of chromosomes and for maintaining the inner centromere
CC       structure.
CC   -!- SUBUNIT: Component of the NMS super complex which consists of mis12,
CC       mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7, spc24 and spc25. Interacts
CC       with dis1, mal2, mis14, ppe1 and ekc1. {ECO:0000269|PubMed:16079914,
CC       ECO:0000269|PubMed:17035632}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
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DR   EMBL; CU329671; CAB52611.1; -; Genomic_DNA.
DR   PIR; T40436; T40436.
DR   RefSeq; NP_595459.1; NM_001021369.2.
DR   AlphaFoldDB; Q9UUB5; -.
DR   SMR; Q9UUB5; -.
DR   BioGRID; 277562; 9.
DR   IntAct; Q9UUB5; 1.
DR   STRING; 4896.SPBC409.09c.1; -.
DR   iPTMnet; Q9UUB5; -.
DR   MaxQB; Q9UUB5; -.
DR   PaxDb; Q9UUB5; -.
DR   EnsemblFungi; SPBC409.09c.1; SPBC409.09c.1:pep; SPBC409.09c.
DR   GeneID; 2541047; -.
DR   KEGG; spo:SPBC409.09c; -.
DR   PomBase; SPBC409.09c; mis13.
DR   VEuPathDB; FungiDB:SPBC409.09c; -.
DR   eggNOG; ENOG502S2VJ; Eukaryota.
DR   HOGENOM; CLU_052069_0_0_1; -.
DR   InParanoid; Q9UUB5; -.
DR   OMA; IPNKPHP; -.
DR   PhylomeDB; Q9UUB5; -.
DR   Reactome; R-SPO-6798695; Neutrophil degranulation.
DR   PRO; PR:Q9UUB5; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR   GO; GO:0000939; C:inner kinetochore; IDA:PomBase.
DR   GO; GO:0031617; C:NMS complex; IDA:PomBase.
DR   GO; GO:0000818; C:nuclear MIS12/MIND complex; ISM:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR   GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR   InterPro; IPR013218; Dsn1/Mis13.
DR   PANTHER; PTHR14778; PTHR14778; 1.
DR   Pfam; PF08202; MIS13; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Meiosis; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..329
FT                   /note="Kinetochore protein mis13"
FT                   /id="PRO_0000290643"
FT   REGION          1..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          228..249
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..34
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        51..72
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   329 AA;  36798 MW;  7A85BDC557C8C04D CRC64;
     MKRKPEEQEG FVFVRKGKEK AGSKRRKSSV EDDPDTSQTD GLVELPVSDT PIVKRNKELR
     KGKGRRSSLD QRGKRASSIG TGFEALPHAD VPSHEYYRHI SKDLSEPLRI KQLLLWASSK
     ALEEQRKKYG ETEEASEAAI ARSIVQEVLN ELLANKVSVS WYQRPPDAVI PNKPHPQNLK
     NAQLVDELSA KLTQLHNEEA AWRAVAANSV SSDKSILSFK KAVESIDSKQ DLDKQDSPLP
     PDDAPELPNI SKLKPKFHTL LDMLAENIHT LHSLTNAGPE VRSSYGRLAA QDFIAHRKSL
     LSFSKYVDTM NLLRLLSEAS YKSSSNESV