MIS14_SCHPO
ID MIS14_SCHPO Reviewed; 210 AA.
AC Q9P6M3;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Kinetochore protein mis14;
DE AltName: Full=NMS complex subunit mis14;
GN Name=mis14; ORFNames=SPAC688.02c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, INTERACTION WITH MIS12, AND SUBCELLULAR LOCATION.
RX PubMed=15369671; DOI=10.1016/j.cell.2004.09.002;
RA Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M.;
RT "Mis16 and Mis18 are required for CENP-A loading and histone deacetylation
RT at centromeres.";
RL Cell 118:715-729(2004).
RN [3]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [4]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT "Reconstruction of the kinetochore during meiosis in fission yeast
RT Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 17:5173-5184(2006).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super
CC complex which has a role in kinetochore function during late meiotic
CC prophase and throughout the mitotic cell cycle. Required for correct
CC chromosome segregation where it has a role in the formation and/or
CC maintenance of specialized chromatin at the centromere.
CC {ECO:0000269|PubMed:15369671}.
CC -!- SUBUNIT: Component of the NMS super complex which consists of mis12,
CC mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7, spc24 and spc25. Interacts
CC with mis12. {ECO:0000269|PubMed:15369671, ECO:0000269|PubMed:16079914,
CC ECO:0000269|PubMed:17035632}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome,
CC centromere, kinetochore.
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DR EMBL; CU329670; CAB90769.1; -; Genomic_DNA.
DR RefSeq; NP_594061.1; NM_001019485.2.
DR AlphaFoldDB; Q9P6M3; -.
DR SMR; Q9P6M3; -.
DR BioGRID; 279902; 8.
DR IntAct; Q9P6M3; 1.
DR STRING; 4896.SPAC688.02c.1; -.
DR MaxQB; Q9P6M3; -.
DR PaxDb; Q9P6M3; -.
DR EnsemblFungi; SPAC688.02c.1; SPAC688.02c.1:pep; SPAC688.02c.
DR GeneID; 2543482; -.
DR KEGG; spo:SPAC688.02c; -.
DR PomBase; SPAC688.02c; mis14.
DR VEuPathDB; FungiDB:SPAC688.02c; -.
DR eggNOG; ENOG502SA1D; Eukaryota.
DR HOGENOM; CLU_1240755_0_0_1; -.
DR InParanoid; Q9P6M3; -.
DR OMA; GMRQNIS; -.
DR PhylomeDB; Q9P6M3; -.
DR PRO; PR:Q9P6M3; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000939; C:inner kinetochore; TAS:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0000444; C:MIS12/MIND type complex; IBA:GO_Central.
DR GO; GO:0031617; C:NMS complex; IDA:PomBase.
DR GO; GO:0000818; C:nuclear MIS12/MIND complex; IPI:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR InterPro; IPR013950; Mis14/Nsl1.
DR PANTHER; PTHR31749; PTHR31749; 1.
DR Pfam; PF08641; Mis14; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Kinetochore; Meiosis;
KW Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..210
FT /note="Kinetochore protein mis14"
FT /id="PRO_0000278390"
SQ SEQUENCE 210 AA; 24122 MW; 400DC20681812390 CRC64;
MSSHGDASVL PKIALESIND YSYVKSVFRQ AIQEKLAIHL PEQASREAGM GREDQLRIRV
KEMLNELIES TFSIIQENVT INGFDANSAL QDPKNSDQIE PFDLALRTRV QQLFNEVEDA
HVLVARYRKS VPAQYEKAYV DAMEQQTAFL RNVKDDYVSL QDKEVENPDE QTSTTVFRKE
DIDRYEQTIA KVAYLKKNLP RVVARLEKTP
