MIS19_SCHPO
ID MIS19_SCHPO Reviewed; 112 AA.
AC O42995;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=CENP-A recruiting complex protein mis19 {ECO:0000305};
DE AltName: Full=Eighteen-interacting centromere protein 1 {ECO:0000303|PubMed:24789708};
DE AltName: Full=Kinetochore protein mis19 {ECO:0000305};
GN Name=mis19 {ECO:0000303|PubMed:24774534};
GN Synonyms=eic1 {ECO:0000303|PubMed:24789708},
GN kis1 {ECO:0000303|PubMed:25375240}; ORFNames=SPBC27B12.02, SPBC30B4.10;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP FUNCTION, IDENTIFICATION IN THE CENP-A RECRUITING COMPLEX, SUBCELLULAR
RP LOCATION, AND MUTAGENESIS OF PHE-32.
RX PubMed=24774534; DOI=10.1111/gtc.12152;
RA Hayashi T., Ebe M., Nagao K., Kokubu A., Sajiki K., Yanagida M.;
RT "Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18
RT to recruit CENP-A through interacting with NMD factors and the SWI/SNF
RT complex.";
RL Genes Cells 19:541-554(2014).
RN [4]
RP FUNCTION, IDENTIFICATION IN THE CENP-A RECRUITING COMPLEX, AND SUBCELLULAR
RP LOCATION.
RX PubMed=24789708; DOI=10.1098/rsob.140043;
RA Subramanian L., Toda N.R., Rappsilber J., Allshire R.C.;
RT "Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A
RT assembly.";
RL Open Biol. 4:140043-140043(2014).
RN [5]
RP FUNCTION, IDENTIFICATION IN THE CENP-A RECRUITING COMPLEX, SUBCELLULAR
RP LOCATION, AND MUTAGENESIS OF ARG-65.
RX PubMed=25375240; DOI=10.1371/journal.pone.0111905;
RA Hirai H., Arai K., Kariyazono R., Yamamoto M., Sato M.;
RT "The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic
RT spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-
RT A.";
RL PLoS ONE 9:E111905-E111905(2014).
CC -!- FUNCTION: Component of the CENP-A recruiting complex that ensures the
CC integrity of mitotic spindles through maintenance of kinetochore
CC factors mis6/CENP-I and cnp1/CENP-A (PubMed:24774534, PubMed:24789708,
CC PubMed:25375240). Links mis16 and mis18 to recruit CENP-A through
CC interacting with non-sense-mediated mRNA decay (NMD) factors and the
CC SWI/SNF complex (PubMed:24774534). Links also mis18 with the
CC CCAN/mis6/ctf19 complex to promote CENP-A assembly (PubMed:24789708).
CC {ECO:0000269|PubMed:24774534, ECO:0000269|PubMed:24789708,
CC ECO:0000269|PubMed:25375240}.
CC -!- SUBUNIT: Component of the CENP-A recruiting complex composed of at
CC least mis16, mis19, mis19 and mis20 (PubMed:24774534, PubMed:24789708,
CC PubMed:25375240). {ECO:0000269|PubMed:24774534,
CC ECO:0000269|PubMed:24789708, ECO:0000269|PubMed:25375240}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:24774534, ECO:0000269|PubMed:24789708,
CC ECO:0000269|PubMed:25375240}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:25375240}. Note=Localizes at the centromeres during
CC interphase, but not in mitosis (PubMed:24774534, PubMed:25375240).
CC {ECO:0000269|PubMed:24774534, ECO:0000269|PubMed:25375240}.
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DR EMBL; CU329671; CAB63198.2; -; Genomic_DNA.
DR PIR; T40026; T40026.
DR RefSeq; NP_595535.2; NM_001021445.2.
DR PDB; 5WJC; X-ray; 2.30 A; B=1-112.
DR PDB; 6S29; X-ray; 1.99 A; B/D=52-112.
DR PDBsum; 5WJC; -.
DR PDBsum; 6S29; -.
DR AlphaFoldDB; O42995; -.
DR SMR; O42995; -.
DR BioGRID; 276898; 17.
DR STRING; 4896.SPBC27B12.02.1; -.
DR MaxQB; O42995; -.
DR PaxDb; O42995; -.
DR EnsemblFungi; SPBC27B12.02.1; SPBC27B12.02.1:pep; SPBC27B12.02.
DR GeneID; 2540369; -.
DR KEGG; spo:SPBC27B12.02; -.
DR PomBase; SPBC27B12.02; mis19.
DR VEuPathDB; FungiDB:SPBC27B12.02; -.
DR HOGENOM; CLU_2110385_0_0_1; -.
DR OMA; RANSWTQ; -.
DR PRO; PR:O42995; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0098654; C:CENP-A recruiting complex; IDA:PomBase.
DR GO; GO:0034506; C:chromosome, centromeric core domain; EXP:PomBase.
DR GO; GO:0000776; C:kinetochore; EXP:PomBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:PomBase.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR GO; GO:0071459; P:protein localization to chromosome, centromeric region; IMP:PomBase.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Centromere; Chromosome;
KW Kinetochore; Mitosis; Reference proteome.
FT CHAIN 1..112
FT /note="CENP-A recruiting complex protein mis19"
FT /id="PRO_0000116509"
FT MUTAGEN 32
FT /note="F->I: In mis19-1: Leads to a chromosome
FT missegregation phenotype that results in large and small
FT daughter nuclei."
FT /evidence="ECO:0000269|PubMed:24774534"
FT MUTAGEN 65
FT /note="R->C: In kis1-1: Leads to defective kinetochore-
FT microtubule attachment."
FT /evidence="ECO:0000269|PubMed:25375240"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:6S29"
FT HELIX 79..97
FT /evidence="ECO:0007829|PDB:6S29"
FT HELIX 104..111
FT /evidence="ECO:0007829|PDB:6S29"
SQ SEQUENCE 112 AA; 13426 MW; 9684034F30AB2853 CRC64;
MDLMPLEKAR AIEIAFDNVF HNTKIPDNLQ QFDAILKRLE RRRFIPTENQ KPRVYETELL
VLRFREFGVK DNHNHPINLH SLRSKSLIRA QGKKLDLHNR VFLRRNVRAV KM
