MIS20_SCHPO
ID MIS20_SCHPO Reviewed; 253 AA.
AC O94682;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=CENP-A recruiting complex protein mis20 {ECO:0000305};
DE AltName: Full=Eighteen-interacting centromere protein 2 {ECO:0000303|PubMed:24789708};
DE AltName: Full=Kinetochore protein mis20 {ECO:0000305};
GN Name=mis20 {ECO:0000303|PubMed:24774534};
GN Synonyms=eic2 {ECO:0000303|PubMed:24789708}; ORFNames=SPBC776.16;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP FUNCTION, IDENTIFICATION IN THE CENP-A RECRUITING COMPLEX, AND SUBCELLULAR
RP LOCATION.
RX PubMed=24774534; DOI=10.1111/gtc.12152;
RA Hayashi T., Ebe M., Nagao K., Kokubu A., Sajiki K., Yanagida M.;
RT "Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18
RT to recruit CENP-A through interacting with NMD factors and the SWI/SNF
RT complex.";
RL Genes Cells 19:541-554(2014).
RN [4]
RP FUNCTION, IDENTIFICATION IN THE CENP-A RECRUITING COMPLEX, AND SUBCELLULAR
RP LOCATION.
RX PubMed=24789708; DOI=10.1098/rsob.140043;
RA Subramanian L., Toda N.R., Rappsilber J., Allshire R.C.;
RT "Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A
RT assembly.";
RL Open Biol. 4:140043-140043(2014).
CC -!- FUNCTION: Component of the CENP-A recruiting complex that ensures the
CC integrity of mitotic spindles through maintenance of kinetochore
CC factors mis6/CENP-I and cnp1/CENP-A (PubMed:24774534, PubMed:24789708).
CC Seems dispensable for proper chromosome segregation (PubMed:24774534).
CC {ECO:0000269|PubMed:24774534, ECO:0000269|PubMed:24789708}.
CC -!- SUBUNIT: Component of the CENP-A recruiting complex composed of at
CC least mis16, mis19, mis19 and mis20 (PubMed:24774534, PubMed:24789708).
CC {ECO:0000269|PubMed:24774534, ECO:0000269|PubMed:24789708}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole
CC body {ECO:0000269|PubMed:16823372}. Chromosome, centromere
CC {ECO:0000269|PubMed:24774534, ECO:0000269|PubMed:24789708}.
CC Note=Localizes at the centromeres during interphase, but not in mitosis
CC (PubMed:24774534). {ECO:0000269|PubMed:24774534}.
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DR EMBL; CU329671; CAA22889.1; -; Genomic_DNA.
DR PIR; T40687; T40687.
DR RefSeq; NP_596332.1; NM_001022253.2.
DR AlphaFoldDB; O94682; -.
DR SMR; O94682; -.
DR BioGRID; 277649; 9.
DR STRING; 4896.SPBC776.16.1; -.
DR iPTMnet; O94682; -.
DR PaxDb; O94682; -.
DR PRIDE; O94682; -.
DR EnsemblFungi; SPBC776.16.1; SPBC776.16.1:pep; SPBC776.16.
DR PomBase; SPBC776.16; mis20.
DR VEuPathDB; FungiDB:SPBC776.16; -.
DR HOGENOM; CLU_1099038_0_0_1; -.
DR PRO; PR:O94682; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0098654; C:CENP-A recruiting complex; IDA:PomBase.
DR GO; GO:0034506; C:chromosome, centromeric core domain; EXP:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:PomBase.
DR GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; NAS:PomBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW Mitosis; Reference proteome.
FT CHAIN 1..253
FT /note="CENP-A recruiting complex protein mis20"
FT /id="PRO_0000304095"
FT REGION 113..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..133
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 253 AA; 28725 MW; A979AA30FADDBC8A CRC64;
MLEQSESHAF INNAPKEDRI QVKFEQLFES LPLPLRAEEA LSKLRHDSAR LMILKTSDPT
LNMSTYSIED SPMGFECLKY NLSDNNKLLS QNNYRLPDYL EEDEIVSYTF SKTGPTTSKN
KHPSHSNTIR SPPYKVKKES CHTEINNVSN VSTESINVID ASRGYSPYTS VDSLSVSKNR
SFISLEESAS NQYDAAEAFY FNADSSSPLR KLSPIELPVT PIRRKTPTIN PNSELKRLQT
FGKLILHKGS RRR