MIX14_YEAST
ID MIX14_YEAST Reviewed; 121 AA.
AC Q04341; D6VS15; Q07789;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Mitochondrial intermembrane space cysteine motif-containing protein MIX14;
DE AltName: Full=Mitochondrial intermembrane space CX(n)C motif protein of 14 kDa;
GN Name=MIX14; Synonyms=MIC14; OrderedLocusNames=YDR031W; ORFNames=YD9673.01;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=17095012; DOI=10.1016/j.jmb.2006.10.038;
RA Gabriel K., Milenkovic D., Chacinska A., Mueller J., Guiard B., Pfanner N.,
RA Meisinger C.;
RT "Novel mitochondrial intermembrane space proteins as substrates of the MIA
RT import pathway.";
RL J. Mol. Biol. 365:612-620(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=22984289; DOI=10.1074/mcp.m112.021105;
RA Voegtle F.N., Burkhart J.M., Rao S., Gerbeth C., Hinrichs J.,
RA Martinou J.C., Chacinska A., Sickmann A., Zahedi R.P., Meisinger C.;
RT "Intermembrane space proteome of yeast mitochondria.";
RL Mol. Cell. Proteomics 11:1840-1852(2012).
RN [5]
RP GENE NAME.
RX PubMed=24687277; DOI=10.1083/jcb.201401006;
RA Pfanner N., van der Laan M., Amati P., Capaldi R.A., Caudy A.A.,
RA Chacinska A., Darshi M., Deckers M., Hoppins S., Icho T., Jakobs S., Ji J.,
RA Kozjak-Pavlovic V., Meisinger C., Odgren P.R., Park S.K., Rehling P.,
RA Reichert A.S., Sheikh M.S., Taylor S.S., Tsuchida N., van der Bliek A.M.,
RA van der Klei I.J., Weissman J.S., Westermann B., Zha J., Neupert W.,
RA Nunnari J.;
RT "Uniform nomenclature for the mitochondrial contact site and cristae
RT organizing system.";
RL J. Cell Biol. 204:1083-1086(2014).
CC -!- SUBCELLULAR LOCATION: Mitochondrion intermembrane space
CC {ECO:0000269|PubMed:17095012, ECO:0000269|PubMed:22984289}.
CC Note=Imported into mitochondrion intermembrane space through the MIA
CC pathway. {ECO:0000269|PubMed:17095012}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA98853.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Z68196; CAA92368.1; -; Genomic_DNA.
DR EMBL; Z74327; CAA98853.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006938; DAA11875.1; -; Genomic_DNA.
DR PIR; S67845; S67845.
DR RefSeq; NP_010314.4; NM_001180339.3.
DR AlphaFoldDB; Q04341; -.
DR SMR; Q04341; -.
DR BioGRID; 32080; 28.
DR DIP; DIP-5131N; -.
DR IntAct; Q04341; 1.
DR STRING; 4932.YDR031W; -.
DR MaxQB; Q04341; -.
DR PaxDb; Q04341; -.
DR PRIDE; Q04341; -.
DR EnsemblFungi; YDR031W_mRNA; YDR031W; YDR031W.
DR GeneID; 851595; -.
DR KEGG; sce:YDR031W; -.
DR SGD; S000002438; MIX14.
DR VEuPathDB; FungiDB:YDR031W; -.
DR eggNOG; ENOG502S4BW; Eukaryota.
DR HOGENOM; CLU_160816_0_0_1; -.
DR InParanoid; Q04341; -.
DR OMA; QFLAFHQ; -.
DR BioCyc; YEAST:G3O-29646-MON; -.
DR PRO; PR:Q04341; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04341; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0009060; P:aerobic respiration; IMP:SGD.
DR GO; GO:0045333; P:cellular respiration; IBA:GO_Central.
DR InterPro; IPR031731; CX9C.
DR InterPro; IPR016611; Mix14.
DR Pfam; PF16860; CX9C; 2.
DR PIRSF; PIRSF013232; UCP013232; 1.
DR PROSITE; PS51808; CHCH; 2.
PE 1: Evidence at protein level;
KW Disulfide bond; Mitochondrion; Reference proteome; Repeat.
FT CHAIN 1..121
FT /note="Mitochondrial intermembrane space cysteine motif-
FT containing protein MIX14"
FT /id="PRO_0000242621"
FT DOMAIN 14..56
FT /note="CHCH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DOMAIN 60..105
FT /note="CHCH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 17..27
FT /note="Cx9C motif 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 38..48
FT /note="Cx9C motif 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 63..73
FT /note="Cx9C motif 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 87..97
FT /note="Cx9C motif 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 17..48
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 27..38
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 63..97
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 73..87
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
SQ SEQUENCE 121 AA; 13866 MW; 9502632E07FC7916 CRC64;
MSDILDEIVI EDVVANCPQE FLQYHKCIRD NEENPGKCKD GRMILSTCIR EKVPSVKSIM
SECSEPMKKY DQCIRDNMGT RTINENCLGF LQDLRKCAEL QVKNKNIKPS INGVNLELIK
D
