MIX17_YEAST
ID MIX17_YEAST Reviewed; 156 AA.
AC Q03667; D6VZH7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Mitochondrial intermembrane space cysteine motif-containing protein MIX17;
DE AltName: Full=Mitochondrial intermembrane space CX(n)C motif protein of 17 kDa;
DE Flags: Precursor;
GN Name=MIX17; Synonyms=MIC17; OrderedLocusNames=YMR002W; ORFNames=YM8270.04;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=17095012; DOI=10.1016/j.jmb.2006.10.038;
RA Gabriel K., Milenkovic D., Chacinska A., Mueller J., Guiard B., Pfanner N.,
RA Meisinger C.;
RT "Novel mitochondrial intermembrane space proteins as substrates of the MIA
RT import pathway.";
RL J. Mol. Biol. 365:612-620(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=22984289; DOI=10.1074/mcp.m112.021105;
RA Voegtle F.N., Burkhart J.M., Rao S., Gerbeth C., Hinrichs J.,
RA Martinou J.C., Chacinska A., Sickmann A., Zahedi R.P., Meisinger C.;
RT "Intermembrane space proteome of yeast mitochondria.";
RL Mol. Cell. Proteomics 11:1840-1852(2012).
RN [7]
RP GENE NAME.
RX PubMed=24687277; DOI=10.1083/jcb.201401006;
RA Pfanner N., van der Laan M., Amati P., Capaldi R.A., Caudy A.A.,
RA Chacinska A., Darshi M., Deckers M., Hoppins S., Icho T., Jakobs S., Ji J.,
RA Kozjak-Pavlovic V., Meisinger C., Odgren P.R., Park S.K., Rehling P.,
RA Reichert A.S., Sheikh M.S., Taylor S.S., Tsuchida N., van der Bliek A.M.,
RA van der Klei I.J., Weissman J.S., Westermann B., Zha J., Neupert W.,
RA Nunnari J.;
RT "Uniform nomenclature for the mitochondrial contact site and cristae
RT organizing system.";
RL J. Cell Biol. 204:1083-1086(2014).
CC -!- SUBCELLULAR LOCATION: Mitochondrion intermembrane space
CC {ECO:0000269|PubMed:17095012, ECO:0000269|PubMed:22984289}.
CC Note=Imported into mitochondrion intermembrane space through the MIA
CC pathway.
CC -!- MISCELLANEOUS: Present with 6960 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z48613; CAA88517.1; -; Genomic_DNA.
DR EMBL; AY557766; AAS56092.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09901.1; -; Genomic_DNA.
DR PIR; S53031; S53031.
DR RefSeq; NP_013715.1; NM_001182498.1.
DR AlphaFoldDB; Q03667; -.
DR SMR; Q03667; -.
DR BioGRID; 35172; 56.
DR DIP; DIP-2627N; -.
DR IntAct; Q03667; 2.
DR MINT; Q03667; -.
DR STRING; 4932.YMR002W; -.
DR iPTMnet; Q03667; -.
DR MaxQB; Q03667; -.
DR PaxDb; Q03667; -.
DR PRIDE; Q03667; -.
DR EnsemblFungi; YMR002W_mRNA; YMR002W; YMR002W.
DR GeneID; 855014; -.
DR KEGG; sce:YMR002W; -.
DR SGD; S000004604; MIX17.
DR VEuPathDB; FungiDB:YMR002W; -.
DR eggNOG; KOG4090; Eukaryota.
DR GeneTree; ENSGT00940000172340; -.
DR HOGENOM; CLU_093520_0_1_1; -.
DR InParanoid; Q03667; -.
DR OMA; CDADARN; -.
DR BioCyc; YEAST:G3O-32713-MON; -.
DR PRO; PR:Q03667; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q03667; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0009060; P:aerobic respiration; IMP:SGD.
DR GO; GO:0007005; P:mitochondrion organization; IBA:GO_Central.
PE 1: Evidence at protein level;
KW Disulfide bond; Mitochondrion; Reference proteome; Transit peptide.
FT TRANSIT 1..21
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 22..156
FT /note="Mitochondrial intermembrane space cysteine motif-
FT containing protein MIX17"
FT /id="PRO_0000203267"
FT DOMAIN 115..156
FT /note="CHCH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 78..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 118..128
FT /note="Cx9C motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT COMPBIAS 1..45
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 118..149
FT /evidence="ECO:0000250"
FT DISULFID 128..139
FT /evidence="ECO:0000250"
SQ SEQUENCE 156 AA; 16669 MW; 3FFA2980FDE3A6BF CRC64;
MARSRGSSRP ISRSRPTQTR SASTMAAPVH PQQQQQPNAY SHPPAAGAQT RQPGMFAQMA
STAAGVAVGS TIGHTLGAGI TGMFSGSGSD SAPVEQQQQN MANTSGQTQT DQQLGRTCEI
DARNFTRCLD ENNGNFQICD YYLQQLKACQ EAARQY
