ID   MIX1_XENLA              Reviewed;         377 AA.
AC   P21711;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Homeobox protein Mix.1;
GN   Name=mix-a; Synonyms=mix1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC   TISSUE=Gastrula;
RX   PubMed=2567635; DOI=10.1016/0092-8674(89)90335-8;
RA   Rosa F.M.;
RT   "Mix.1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly
RT   in the presumptive endodermal cells of Xenopus embryos.";
RL   Cell 57:965-974(1989).
RN   [2]
RP   FUNCTION.
RX   PubMed=12710967; DOI=10.1016/s0012-1606(03)00034-4;
RA   Yamamoto S., Hikasa H., Ono H., Taira M.;
RT   "Molecular link in the sequential induction of the Spemann organizer:
RT   direct activation of the cerberus gene by Xlim-1, Xotx2, Mix.1, and
RT   Siamois, immediately downstream from Nodal and Wnt signaling.";
RL   Dev. Biol. 257:190-204(2003).
CC   -!- FUNCTION: Transcription factor which plays a regulatory role in the
CC       development of the embryo. Involved in the establishment of
CC       dorsal/ventral pattern in the early mesoderm. Activates the head
CC       organizer gene cer1 by acting synergistically with otx2 and siamois
CC       through the 5'-TAATCT-3' element of the cer1 promoter. Also binds as a
CC       complex with lhx1/lim1 and siamois to the 3x 5'-TAAT-3' element of the
CC       cer1 promoter. {ECO:0000269|PubMed:12710967,
CC       ECO:0000269|PubMed:2567635}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed mostly in the presumptive endodermal
CC       cells of Xenopus embryos. {ECO:0000269|PubMed:2567635}.
CC   -!- INDUCTION: By activin and TGF-beta1 (immediate early response gene).
CC       {ECO:0000269|PubMed:2567635}.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR   EMBL; M27063; AAA49903.1; -; mRNA.
DR   PIR; A32548; A32548.
DR   RefSeq; NP_001081294.1; NM_001087825.1.
DR   AlphaFoldDB; P21711; -.
DR   SMR; P21711; -.
DR   IntAct; P21711; 1.
DR   GeneID; 397759; -.
DR   KEGG; xla:397759; -.
DR   CTD; 397759; -.
DR   Xenbase; XB-GENE-865559; mix1.S.
DR   OMA; TQGGTYG; -.
DR   OrthoDB; 1270742at2759; -.
DR   Proteomes; UP000186698; Chromosome 5S.
DR   Bgee; 397759; Expressed in gastrula and 1 other tissue.
DR   GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:BHF-UCL.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..377
FT                   /note="Homeobox protein Mix.1"
FT                   /id="PRO_0000049193"
FT   DNA_BIND        96..155
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          41..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          334..369
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   377 AA;  42250 MW;  6BA404359B57D221 CRC64;
     MDGFSQQLED LYPSCFSPCP SPLGFSEPVI QPFAMNLAPA AQKDFQQHPN RREVTKIPGA
     GEQSPVQNVR PKDAINPKEA DPRSPTADAS LVPASQRRKR TFFTQAQLDI LEQFFQTNMY
     PDIHHREELA RHIYIPESRI QVWFQNRRAK VRRQGAKATK PILAGHHYSG TSGANRAMFP
     SAPAPNSSSH QMTTSRAQVQ PLKESQMNMF HQNQGFLPYP DSSCNVSRQR FLMSQPTPGA
     YHLPQASSNV YNQNVKSQNP LWNQQTTNRV YTNMMETVLD LSRRPSQQMP VQPMLMNSFQ
     TNKNIKPEVY TTSPQIPVST TSSQVSLFAN QEPCHMSTTQ GGTYGQISPI SDSGVSDTSP
     EPSSDWEENV ASVLLNL