ID   MJE1_VITVI              Reviewed;         261 AA.
AC   D7SSD8; A0A438FCE1;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Methyl jasmonate esterase 1 {ECO:0000303|PubMed:26934101};
DE            Short=VvMJE1 {ECO:0000303|PubMed:26934101};
DE            EC=3.1.1.- {ECO:0000269|PubMed:26934101};
DE   AltName: Full=Methyl esterase 5 {ECO:0000303|PubMed:26934101};
DE            Short=VvMES5 {ECO:0000303|PubMed:26934101};
DE   AltName: Full=Salicylic acid-binding protein 2-4 {ECO:0000312|EMBL:RVW57636.1};
GN   Name=MJE1 {ECO:0000303|PubMed:26934101};
GN   Synonyms=MES5 {ECO:0000303|PubMed:26934101},
GN   SABP2_4 {ECO:0000312|EMBL:RVW57636.1};
GN   ORFNames=CK203_096003 {ECO:0000312|EMBL:RVW57636.1},
GN   VIT_00s0253g00150 {ECO:0000312|EMBL:CBI18570.3};
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; Vitales; Vitaceae; Viteae; Vitis.
OX   NCBI_TaxID=29760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP   BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND INDUCTION BY COLD AND UV-B.
RC   STRAIN=cv. Chardonnay; TISSUE=Leaf;
RX   PubMed=26934101; DOI=10.1016/j.plaphy.2016.02.027;
RA   Zhao N., Lin H., Lan S., Jia Q., Chen X., Guo H., Chen F.;
RT   "VvMJE1 of the grapevine (Vitis vinifera) VvMES methylesterase family
RT   encodes for methyl jasmonate esterase and has a role in stress response.";
RL   Plant Physiol. Biochem. 102:125-132(2016).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Pinot noir / PN40024;
RX   PubMed=17721507; DOI=10.1038/nature06148;
RA   Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA   Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA   Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA   Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA   Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA   Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA   Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA   Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA   Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA   Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA   Wincker P.;
RT   "The grapevine genome sequence suggests ancestral hexaploidization in major
RT   angiosperm phyla.";
RL   Nature 449:463-467(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Chardonnay; TISSUE=Leaf;
RX   PubMed=30458008; DOI=10.1371/journal.pgen.1007807;
RA   Roach M.J., Johnson D.L., Bohlmann J., van Vuuren H.J., Jones S.J.,
RA   Pretorius I.S., Schmidt S.A., Borneman A.R.;
RT   "Population sequencing reveals clonal diversity and ancestral inbreeding in
RT   the grapevine cultivar Chardonnay.";
RL   PLoS Genet. 14:e1007807-e1007807(2018).
CC   -!- FUNCTION: Methylesterase that catalyzes the hydrolysis of methyl
CC       jasmonate (MeJA) into jasmonate (JA) (PubMed:26934101). Can also use
CC       methyl salicylate (MeSA) as substrate with a lower efficiency
CC       (PubMed:26934101). {ECO:0000269|PubMed:26934101}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + methyl (-)-jasmonate = H(+) + jasmonate + methanol;
CC         Xref=Rhea:RHEA:55372, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15929, ChEBI:CHEBI:17790, ChEBI:CHEBI:58431;
CC         Evidence={ECO:0000269|PubMed:26934101};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55373;
CC         Evidence={ECO:0000269|PubMed:26934101};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + methyl salicylate = H(+) + methanol + salicylate;
CC         Xref=Rhea:RHEA:33611, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17790, ChEBI:CHEBI:30762, ChEBI:CHEBI:31832;
CC         Evidence={ECO:0000269|PubMed:26934101};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33612;
CC         Evidence={ECO:0000269|PubMed:26934101};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=92.9 uM for methyl jasmonate {ECO:0000269|PubMed:26934101};
CC   -!- PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:26934101}.
CC   -!- PATHWAY: Lipid metabolism; oxylipin biosynthesis.
CC       {ECO:0000269|PubMed:26934101}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q9SE93}.
CC   -!- INDUCTION: By cold and UV-B. {ECO:0000269|PubMed:26934101}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase
CC       family. {ECO:0000305}.
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DR   EMBL; FN594975; CBI18570.3; -; Genomic_DNA.
DR   EMBL; QGNW01001049; RVW57636.1; -; Genomic_DNA.
DR   RefSeq; XP_010646594.1; XM_010648292.1.
DR   STRING; 29760.VIT_00s0253g00150.t01; -.
DR   ESTHER; vitvi-d7ssd8; Hydroxynitrile_lyase.
DR   GeneID; 100253882; -.
DR   KEGG; vvi:100253882; -.
DR   eggNOG; ENOG502QVWZ; Eukaryota.
DR   HOGENOM; CLU_046066_0_1_1; -.
DR   InParanoid; D7SSD8; -.
DR   OMA; HEVKMIN; -.
DR   OrthoDB; 923240at2759; -.
DR   UniPathway; UPA00382; -.
DR   Proteomes; UP000009183; Unassembled WGS sequence, unordered.
DR   Proteomes; UP000288805; Unassembled WGS sequence.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IBA:GO_Central.
DR   GO; GO:0080032; F:methyl jasmonate esterase activity; IDA:UniProtKB.
DR   GO; GO:0080031; F:methyl salicylate esterase activity; IBA:GO_Central.
DR   GO; GO:0009694; P:jasmonic acid metabolic process; IDA:UniProtKB.
DR   GO; GO:0031408; P:oxylipin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009409; P:response to cold; IEP:UniProtKB.
DR   GO; GO:0010224; P:response to UV-B; IEP:UniProtKB.
DR   GO; GO:0009696; P:salicylic acid metabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR045889; MES/HNL.
DR   PANTHER; PTHR10992; PTHR10992; 1.
DR   Pfam; PF12697; Abhydrolase_6; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..261
FT                   /note="Methyl jasmonate esterase 1"
FT                   /id="PRO_0000454242"
FT   DOMAIN          8..251
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        82
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT   ACT_SITE        211
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT   ACT_SITE        239
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6RYA0"
FT   CONFLICT        202
FT                   /note="R -> H (in Ref. 3; RVW57636)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   261 AA;  29486 MW;  736434B97B8E84C6 CRC64;
     MEKRERHFVL VHGACHGAWC WYKVTTFLRS AGHKVTALDL AAAGANGKRL DELNSISDYH
     EPLMKFMTSL VAGEKVILVA HSLGGVSVSV AMERFPQKIS VAVFVSAYMP GPDFNLSTVY
     QELHQRRQGA SKDTQYTFDR GSNNPPTSII FSPEDLAAKL YQLSPPEDLT LATTLMRPTK
     LFRGENLLKE TTVTREKYGT VRRVYIVCDK DNILKEDFQR WMIKNNPSDE VKVIMGSDHM
     PMFSKPLDLC AYLQEIVESY S