MKB_XENLA
ID MKB_XENLA Reviewed; 142 AA.
AC P48531; Q6GQG8;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Midkine-B {ECO:0000305};
DE Short=MK-B;
DE AltName: Full=Pleiotrophic factor-alpha-2 {ECO:0000303|PubMed:7677748};
DE Short=PTF-alpha-2;
DE Short=X-PTF-alpha2;
DE Flags: Precursor;
GN Name=mdk-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Brain;
RX PubMed=7677748; DOI=10.1006/bbrc.1995.2305;
RA Tsujimura A., Yasojima K., Kuboki Y., Suzuki A., Ueno N., Shiokawa K.,
RA Hashimoto-Gotoh T.;
RT "Developmental and differential regulations in gene expression of Xenopus
RT pleiotrophic factors-alpha and -beta.";
RL Biochem. Biophys. Res. Commun. 214:432-439(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Secreted protein that functions as cytokine and growth factor
CC and mediates its signal through cell-surface proteoglycan and non-
CC proteoglycan receptors. Binds cell-surface proteoglycan receptors via
CC their chondroitin sulfate (CS) groups. Thereby regulates many processes
CC like inflammatory response, cell proliferation, cell adhesion, cell
CC growth, cell survival, tissue regeneration, cell differentiation and
CC cell migration (By similarity). Inhibits mesoderm formation and
CC promotes neural formation during development. Plays a role in
CC development of the neuromuscular junction (NMJ). Has antibacterial
CC activity against both Gram-positive and Gram-negative bacteria (By
CC similarity). {ECO:0000250|UniProtKB:P21741,
CC ECO:0000250|UniProtKB:P48530}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: In adults, expression is highest in the brain, eye
CC and bone, with lower expression in the heart and lung. Not expressed in
CC the ovary. In the tailbud stage embryo, expressed in the head and tail
CC regions as well as in the central nervous system (CNS).
CC {ECO:0000269|PubMed:7677748}.
CC -!- SIMILARITY: Belongs to the pleiotrophin family. {ECO:0000305}.
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DR EMBL; D42057; BAA07657.1; -; mRNA.
DR EMBL; BC072776; AAH72776.1; -; mRNA.
DR PIR; JC4273; JC4273.
DR RefSeq; NP_001084019.1; NM_001090550.1.
DR RefSeq; XP_018112064.1; XM_018256575.1.
DR RefSeq; XP_018112065.1; XM_018256576.1.
DR AlphaFoldDB; P48531; -.
DR SMR; P48531; -.
DR PRIDE; P48531; -.
DR DNASU; 399256; -.
DR GeneID; 399256; -.
DR KEGG; xla:399256; -.
DR CTD; 399256; -.
DR Xenbase; XB-GENE-6254190; mdk.L.
DR OMA; ATECAEW; -.
DR OrthoDB; 1489280at2759; -.
DR Proteomes; UP000186698; Chromosome 4L.
DR Bgee; 399256; Expressed in internal ear and 19 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0043395; F:heparan sulfate proteoglycan binding; ISS:UniProtKB.
DR GO; GO:0008201; F:heparin binding; ISS:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; ISS:UniProtKB.
DR GO; GO:0007528; P:neuromuscular junction development; ISS:UniProtKB.
DR GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; ISS:UniProtKB.
DR Gene3D; 2.20.60.10; -; 1.
DR Gene3D; 2.30.90.10; -; 1.
DR InterPro; IPR000762; Midkine_heparin-bd_GF.
DR InterPro; IPR020090; PTN/MK_C_dom.
DR InterPro; IPR038130; PTN/MK_C_dom_sf.
DR InterPro; IPR020091; PTN/MK_diS_sf.
DR InterPro; IPR020089; PTN/MK_N_dom.
DR InterPro; IPR037122; PTN/MK_N_dom_sf.
DR InterPro; IPR020092; PTN_MK_heparin-bd_GF_CS.
DR PANTHER; PTHR13850; PTHR13850; 1.
DR Pfam; PF01091; PTN_MK_C; 1.
DR Pfam; PF05196; PTN_MK_N; 1.
DR PRINTS; PR00269; PTNMIDKINE.
DR SMART; SM00193; PTN; 1.
DR SUPFAM; SSF57288; SSF57288; 2.
DR PROSITE; PS00619; PTN_MK_1; 1.
DR PROSITE; PS00620; PTN_MK_2; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Developmental protein; Disulfide bond;
KW Growth factor; Heparin-binding; Mitogen; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..142
FT /note="Midkine-B"
FT /id="PRO_0000024667"
FT DISULFID 36..60
FT /evidence="ECO:0000250"
FT DISULFID 44..69
FT /evidence="ECO:0000250"
FT DISULFID 51..73
FT /evidence="ECO:0000250"
FT DISULFID 83..115
FT /evidence="ECO:0000250"
FT DISULFID 93..125
FT /evidence="ECO:0000250"
SQ SEQUENCE 142 AA; 15595 MW; BB47060745F88B28 CRC64;
MELRAFCVIL LITILAVSSQ AAKNKKEKGK KGASDCTEWT WGSCIPNSKD CGAGTREGTC
KEETRKLKCK IPCNWKKDFG ADCKYKFENW GECNATTGQK VRSGTLKKAL YNADCQQTVE
AAKPCSLKTK SKSKGKKGKG KE
