MKRN2_DANRE
ID MKRN2_DANRE Reviewed; 414 AA.
AC Q9DFG8; Q6P0Z6;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=E3 ubiquitin-protein ligase makorin-2;
DE EC=2.3.2.27 {ECO:0000250|UniProtKB:Q9ERV1};
DE AltName: Full=RING-type E3 ubiquitin transferase makorin-2 {ECO:0000305};
GN Name=mkrn2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11597136; DOI=10.1006/geno.2001.6627;
RA Gray T.A., Azama K., Whitmore K., Min A., Abe S., Nicholls R.D.;
RT "Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-
RT finger protein, antisense to the raf1 proto-oncogene.";
RL Genomics 77:119-126(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: E3 ubiquitin ligase catalyzing the covalent attachment of
CC ubiquitin moieties onto substrate proteins (By similarity). Inhibits
CC neurogenesis and axis formation during embryonic development by
CC modulating the phosphatidylinositol 3-kinase (PI3K) pathway (By
CC similarity). Acts downstream of PI3K and akt1 to up-regulate gsk3b mRNA
CC expression (By similarity). {ECO:0000250|UniProtKB:B0F0H3,
CC ECO:0000250|UniProtKB:Q9ERV1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q9ERV1};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9ERV1}. Nucleus
CC {ECO:0000250|UniProtKB:Q9ERV1}.
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DR EMBL; AF277172; AAG27597.1; -; mRNA.
DR EMBL; BC065352; AAH65352.1; -; mRNA.
DR RefSeq; NP_694511.1; NM_152979.2.
DR RefSeq; XP_005155725.1; XM_005155668.3.
DR AlphaFoldDB; Q9DFG8; -.
DR STRING; 7955.ENSDARP00000016210; -.
DR PaxDb; Q9DFG8; -.
DR GeneID; 170783; -.
DR KEGG; dre:170783; -.
DR CTD; 23609; -.
DR ZFIN; ZDB-GENE-020213-2; mkrn2.
DR eggNOG; KOG1039; Eukaryota.
DR InParanoid; Q9DFG8; -.
DR OrthoDB; 1388677at2759; -.
DR PhylomeDB; Q9DFG8; -.
DR TreeFam; TF315108; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9DFG8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030274; F:LIM domain binding; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0002862; P:negative regulation of inflammatory response to antigenic stimulus; ISS:UniProtKB.
DR GO; GO:1901223; P:negative regulation of NIK/NF-kappaB signaling; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:1901485; P:positive regulation of transcription factor catabolic process; ISS:UniProtKB.
DR GO; GO:0043491; P:protein kinase B signaling; ISS:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; ISS:UniProtKB.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR026293; Makorin_2.
DR InterPro; IPR045072; MKRN-like.
DR InterPro; IPR041367; Znf-CCCH_4.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR000571; Znf_CCCH.
DR InterPro; IPR036855; Znf_CCCH_sf.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11224; PTHR11224; 1.
DR PANTHER; PTHR11224:SF17; PTHR11224:SF17; 1.
DR Pfam; PF00097; zf-C3HC4; 1.
DR Pfam; PF00642; zf-CCCH; 1.
DR Pfam; PF18044; zf-CCCH_4; 2.
DR SMART; SM00184; RING; 1.
DR SMART; SM00356; ZnF_C3H1; 4.
DR SUPFAM; SSF90229; SSF90229; 3.
DR PROSITE; PS50103; ZF_C3H1; 4.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; Differentiation; Metal-binding;
KW Neurogenesis; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Transferase; Ubl conjugation pathway; Zinc;
KW Zinc-finger.
FT CHAIN 1..414
FT /note="E3 ubiquitin-protein ligase makorin-2"
FT /id="PRO_0000055957"
FT ZN_FING 2..29
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 31..58
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 164..191
FT /note="C3H1-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 237..291
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 320..349
FT /note="C3H1-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 57..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 192..221
FT /note="Makorin-type Cys-His"
FT CONFLICT 89
FT /note="N -> H (in Ref. 2; AAH65352)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="N -> S (in Ref. 1; AAG27597)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="T -> A (in Ref. 2; AAH65352)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 414 AA; 46480 MW; 8D4BB66047A3B3D6 CRC64;
MSTKQVTCRY FLHGVCREGS RCLFSHDLTT SKPSTICKYY QRGACAYGDR CRYDHIKPPG
RGSGAPADHS NRSSSSAGAS APGPGPPANT SKHLKKPLVL RDKALCSDSR PRVFSAESSE
LNECWEQRDD GAQKPHSYLE AIRSGLDASA AAAATAGTFP ELQQTSPQIC PFLAAGQCQY
GESCPYLHGE MCEICRQHVL HPHDPEQRAA HEKKCMVAFE MDMERAFAVQ QSQDKVCKIC
LDVVYEKSSP SERRFGILSS CAHTYCLNCI RQWRCVEQLH NQIRKSCPEC RVVSEFVIPS
IYWVEDQEQK NLLIEEFKSG VSKKACKYFD QGRGTCPFGG KCFYMHAYAD GRRAEPDKPR
KQLSAEGNVR FQNSVRLWDF IEEREHRSVP QLEDEVNDLG ELFMQLSGAS DAPH
