MKS1_YEAST
ID MKS1_YEAST Reviewed; 584 AA.
AC P34072; D6W1A3; Q02533;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Negative regulator of RAS-cAMP pathway;
GN Name=MKS1; Synonyms=LYS80; OrderedLocusNames=YNL076W;
GN ORFNames=N2344, N2347;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8701611;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<391::aid-yea921>3.0.co;2-n;
RA Poehlmann R., Philippsen P.;
RT "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV
RT reveals 12 new open reading frames (ORFs) and an ancient duplication of six
RT ORFs.";
RL Yeast 12:391-402(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-584.
RX PubMed=8386801; DOI=10.1007/bf00279524;
RA Matsuura A., Anraku Y.;
RT "Characterization of the MKS1 gene, a new negative regulator of the Ras-
RT cyclic AMP pathway in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 238:6-16(1993).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=YAL6B;
RX PubMed=15665377; DOI=10.1074/mcp.m400219-mcp200;
RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M.,
RA Jensen O.N.;
RT "Quantitative phosphoproteomics applied to the yeast pheromone signaling
RT pathway.";
RL Mol. Cell. Proteomics 4:310-327(2005).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-25; SER-247 AND SER-276, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Negative regulator of Ras-cAMP pathway. Involved in
CC transcriptional regulation of galactose-inducible genes.
CC -!- INTERACTION:
CC P34072; P35169: TOR1; NbExp=3; IntAct=EBI-10978, EBI-19374;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA60181.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X86470; CAA60181.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X86470; CAA60182.1; -; Genomic_DNA.
DR EMBL; Z71352; CAA95950.1; -; Genomic_DNA.
DR EMBL; D13715; BAA02868.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10469.1; -; Genomic_DNA.
DR PIR; S53902; S53902.
DR RefSeq; NP_014323.1; NM_001182914.1.
DR AlphaFoldDB; P34072; -.
DR SMR; P34072; -.
DR BioGRID; 35747; 380.
DR DIP; DIP-6677N; -.
DR IntAct; P34072; 19.
DR MINT; P34072; -.
DR STRING; 4932.YNL076W; -.
DR iPTMnet; P34072; -.
DR MaxQB; P34072; -.
DR PaxDb; P34072; -.
DR PRIDE; P34072; -.
DR EnsemblFungi; YNL076W_mRNA; YNL076W; YNL076W.
DR GeneID; 855648; -.
DR KEGG; sce:YNL076W; -.
DR SGD; S000005020; MKS1.
DR VEuPathDB; FungiDB:YNL076W; -.
DR eggNOG; ENOG502RFNU; Eukaryota.
DR HOGENOM; CLU_023053_0_0_1; -.
DR InParanoid; P34072; -.
DR OMA; MDIPSKN; -.
DR BioCyc; YEAST:G3O-33105-MON; -.
DR PRO; PR:P34072; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P34072; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0031930; P:mitochondria-nucleus signaling pathway; IPI:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IMP:SGD.
DR GO; GO:2000220; P:regulation of pseudohyphal growth; IMP:SGD.
DR InterPro; IPR013860; AreA_GATA.
DR Pfam; PF08550; DUF1752; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..584
FT /note="Negative regulator of RAS-cAMP pathway"
FT /id="PRO_0000096494"
FT REGION 95..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..366
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 551..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..128
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..320
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 25
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 247
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956"
FT MOD_RES 276
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 442
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:15665377,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 518
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000255"
SQ SEQUENCE 584 AA; 65643 MW; 79B0EE5F8DDE0145 CRC64;
MSREAFDVPN IGTNKFLKVT PNLFTPERLN LFDDVELYLT LIKASKCVEQ GERLHNISWR
ILNKAVLKEH NINRSKKRDG VKNIYYVLNP NNKQPIKPKQ AAVKQPPLQK ANLPPTTAKQ
NVLTRPMTSP AIAQGAHDRS LDNPNSTNND VKNDVAPNRQ FSKSTTSGLF SNFADKYQKM
KNVNHVANKE EPQTIITGFD TSTVITKKPL QSRRSRSPFQ HIGDMNMNCI DNETSKSTSP
TLENMGSRKS SFPQKESLFG RPRSYKNDQN GQLSLSKTSS RKGKNKIFFS SEDEDSDWDS
VSNDSEFYAD EDDEEYDDYN EEEADQYYRR QWDKLLFAKN QQNLDSTKSS VSSANTINSN
TSHDPVRKSL LSGLFLSEAN SNSNNHNTAH SEYASKHVSP TPQSSHSNIG PQPQQNPPSA
NGIKQQKPSL KTSNVTALAS LSPPQPSNNE RLSMDIQKDF KTDNESNHLY ESNAPLTAQT
ILPTALSTHM FLPNNIHQQR MAIATGSNTR HRFSRRQSMD IPSKNRNTGF LKTRMEISEE
EKMVRTISRL DNTSIANSNG NGNDDTSNQR TEALGRKTSN GGRI
