ID   MKT1_CRYNH              Reviewed;         768 AA.
AC   J9VT48;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Post-transcriptional regulator MKT1 {ECO:0000305};
DE   AltName: Full=Inactive endonuclease MKT1 {ECO:0000305};
GN   Name=MKT1 {ECO:0000303|PubMed:31681631};
GN   ORFNames=CNAG_04808 {ECO:0000312|EMBL:AFR97408.1};
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN   [1] {ECO:0000312|Proteomes:UP000010091}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
RN   [2] {ECO:0000305}
RP   INTERACTION WITH PBP1, IDENTIFICATION BY MASS SPECTROMETRY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=31681631; DOI=10.3389/fcimb.2019.00355;
RA   Son Y.E., Fu C., Jung W.H., Oh S.H., Kwak J.H., Cardenas M.E., Heitman J.,
RA   Park H.S.;
RT   "Pbp1-Interacting Protein Mkt1 Regulates Virulence and Sexual Reproduction
RT   in Cryptococcus neoformans.";
RL   Front. Cell. Infect. Microbiol. 9:355-355(2019).
CC   -!- FUNCTION: Involved in 3'-UTR mediated RNA regulation (By similarity).
CC       Complexes with PBP1 to promote mRNA interactions with poly(A)-binding
CC       protein (By similarity). {ECO:0000250|UniProtKB:P40850}.
CC   -!- SUBUNIT: Interacts with PBP1. {ECO:0000269|PubMed:31681631}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P40850}.
CC   -!- DISRUPTION PHENOTYPE: Abnormal sexual reproduction; decreases MFalpha1
CC       expression and abolishes hyphal elongation (PubMed:31681631). Decreases
CC       virulence in a mouse intranasal inhalation model for pulmonary
CC       infection (PubMed:31681631). Normal vegetative cell population growth
CC       at high temperature (PubMed:31681631). {ECO:0000269|PubMed:31681631}.
CC   -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. {ECO:0000305}.
CC   -!- CAUTION: Although it belongs to the XPG/RAD2 endonuclease family, only
CC       one of the seven Asp residues involved in Mg(2+) binding are conserved
CC       suggesting that it has no nuclease activity. {ECO:0000305}.
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DR   EMBL; CP003829; AFR97408.1; -; Genomic_DNA.
DR   RefSeq; XP_012052222.1; XM_012196832.1.
DR   AlphaFoldDB; J9VT48; -.
DR   SMR; J9VT48; -.
DR   EnsemblFungi; AFR97408; AFR97408; CNAG_04808.
DR   GeneID; 23888187; -.
DR   VEuPathDB; FungiDB:CNAG_04808; -.
DR   HOGENOM; CLU_378548_0_0_1; -.
DR   Proteomes; UP000010091; Chromosome 10.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0004518; F:nuclease activity; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   CDD; cd09902; H3TH_MKT1; 1.
DR   InterPro; IPR022039; MKT1_C.
DR   InterPro; IPR037314; MKT1_H3TH.
DR   InterPro; IPR022040; MKT1_N.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR006084; XPG/Rad2.
DR   InterPro; IPR006085; XPG_DNA_repair_N.
DR   PANTHER; PTHR11081; PTHR11081; 1.
DR   Pfam; PF12246; MKT1_C; 1.
DR   Pfam; PF12247; MKT1_N; 1.
DR   Pfam; PF00752; XPG_N; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Translation regulation.
FT   CHAIN           1..768
FT                   /note="Post-transcriptional regulator MKT1"
FT                   /id="PRO_0000450880"
SQ   SEQUENCE   768 AA;  86778 MW;  2C1845E8128090E4 CRC64;
     MTIRGLDNYL KERKLIQSCP ISTLANTRLG IDATHYLNHL LTDPNSREPL VAATGGLPLA
     IISKIESDLR ALERHAIKPV FVFAGLPLAS RPPPKGPDIK AERENQIKNE AWALYDEGQA
     YSAVDKLVQF NNGNFVDQRD LLRSIMRLFR HRYVEFVVAP YLGIAQLAYL LQHPKGYIHA
     IYSSSECLMW PVERVITSSD WNNNFQFVEK VRILNDLNLT SEQFLDFGIL AGSSLSRTIP
     LPQSEFSIKN IADLVRHHKS GISVCQNVRQ EPPYKAQFYT ESFWKARLAV KFSLVLTTEG
     ACVPLPTVIT PQQQAFTVQD VPGDLEEIFS PRIPDELYFH ICRGLVSAQV VGWLTSGMII
     EQQPLLETGE YRRFIKDVIT EGPTSPRCTT IALLADILHP DWSKRRINVH YYFDPPYAPV
     RGAFVPFTDA LTQSLIGRCS DWMVPMFNLE MELRRQNSST IDLKLCIGAL ASEELVHRTF
     KPKGDRVLDK KDEVVANSLW RFLEVRGFVQ QNHAHSLIGK ALYAAYTVSR VNDRFQEPIY
     LILELLRAGV LHGGKWGGPE AAPLLGGPSF GDEDEQNSVR LIMRCISVLP LVSRNQQWVG
     PLSQELLAFN AFVRGLSKSL RQLFEAVSVH LLLSGDGRRN RDDYSDIMLS LPFQTDVNTG
     FGILAKSYLD ATSYHNQLEP ITEEMIGTEG AETAKRQAIL FIEENFSSVK APVQELERGF
     RFWDAVMVAI RCLAEEQGPN PKLAQTVVGR DVIEQFERAD KWLKPMRP