ID   ML1A_LACTA              Reviewed;         121 AA.
AC   C0HJV7; A0A1B3Z577;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2018, sequence version 2.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Met-lysine-1a {ECO:0000303|PubMed:27287558};
DE            Short=MLys-1a {ECO:0000303|PubMed:27287558};
DE   Flags: Precursor;
OS   Lachesana tarabaevi (Spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC   Lachesana.
OX   NCBI_TaxID=379576 {ECO:0000303|PubMed:27287558};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 70-120, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT MET-120.
RC   TISSUE=Venom {ECO:0000303|PubMed:27287558};
RX   PubMed=27287558; DOI=10.1042/bcj20160436;
RA   Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA   Vassilevski A.A.;
RT   "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL   Biochem. J. 473:2495-2506(2016).
CC   -!- FUNCTION: Shows no antimicrobial activity against Gram-positive
CC       bacterium B.subtilis B-501 or Gram-negative bacterium E.coli DH5-alpha
CC       at concentrations up to 20 ug/ml. Shows no toxicity towards insect
CC       (S.carnaria) larvae. {ECO:0000269|PubMed:27287558}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27287558}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:27287558}.
CC   -!- MASS SPECTROMETRY: Mass=5951.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27287558};
CC   -!- SIMILARITY: Belongs to the met-lysine family.
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DR   EMBL; KT591334; AOH73456.1; -; mRNA.
DR   AlphaFoldDB; C0HJV7; -.
DR   SMR; C0HJV7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..69
FT                   /evidence="ECO:0000269|PubMed:27287558"
FT                   /id="PRO_0000444425"
FT   PEPTIDE         70..120
FT                   /note="Met-lysine-1a"
FT                   /evidence="ECO:0000269|PubMed:27287558"
FT                   /id="PRO_0000437253"
FT   MOD_RES         120
FT                   /note="Methionine amide"
FT                   /evidence="ECO:0000269|PubMed:27287558"
SQ   SEQUENCE   121 AA;  13557 MW;  84A1CE3123D91919 CRC64;
     MKSFVFALAL IVAFACISES KSDHTGYEEE ENLEDSELTD LVTAALLEEL AEASEMDDLS
     YTEEAGGERM DKLEEMALKL KDKLKTMAEK GAQMGEKLKE MLPKAIEKLK ELTEKMKNKM
     G