ML1B_LACTA
ID ML1B_LACTA Reviewed; 121 AA.
AC C0HJV8; A0A1B3Z578;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 2.
DT 25-MAY-2022, entry version 9.
DE RecName: Full=Met-lysine-1b {ECO:0000303|PubMed:27287558};
DE Short=MLys-1b {ECO:0000303|PubMed:27287558};
DE Flags: Precursor;
OS Lachesana tarabaevi (Spider).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC Lachesana.
OX NCBI_TaxID=379576 {ECO:0000303|PubMed:27287558};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 70-120, FUNCTION,
RP SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT MET-120.
RC TISSUE=Venom;
RX PubMed=27287558; DOI=10.1042/bcj20160436;
RA Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA Vassilevski A.A.;
RT "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL Biochem. J. 473:2495-2506(2016).
CC -!- FUNCTION: Shows no antimicrobial activity against Gram-positive
CC bacterium B.subtilis B-501 or Gram-negative bacterium E.coli DH5-alpha
CC at concentrations up to 20 ug/ml. Shows no toxicity towards insect
CC (S.carnaria) larvae. {ECO:0000269|PubMed:27287558}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27287558}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:27287558}.
CC -!- MASS SPECTROMETRY: Mass=6000; Mass_error=0.5; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:27287558};
CC -!- SIMILARITY: Belongs to the met-lysine family.
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DR EMBL; KT591335; AOH73457.1; -; mRNA.
DR AlphaFoldDB; C0HJV8; -.
DR SMR; C0HJV8; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..69
FT /evidence="ECO:0000269|PubMed:27287558"
FT /id="PRO_0000444426"
FT PEPTIDE 70..120
FT /note="Met-lysine-1b"
FT /evidence="ECO:0000269|PubMed:27287558"
FT /id="PRO_0000437254"
FT MOD_RES 120
FT /note="Methionine amide"
FT /evidence="ECO:0000269|PubMed:27287558"
SQ SEQUENCE 121 AA; 13575 MW; 87F7D48093D9090D CRC64;
MKSFVFALAL IVAFACISES KSDHTGYEEE ENLEDSELTD LVAAALLEEL AEASEMDDLS
YTEEAGGERM DKLEEMALKL KDKLKTMAEK GAQMGEKLKE MLPKAMEKLK ELMEKMKNKM
G