MLAA_ECOLI
ID MLAA_ECOLI Reviewed; 251 AA.
AC P76506; P76941;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Intermembrane phospholipid transport system lipoprotein MlaA {ECO:0000305};
DE Flags: Precursor;
GN Name=mlaA; Synonyms=vacJ; OrderedLocusNames=b2346, JW2343;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP 1-4.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION IN PHOSPHOLIPID TRANSPORT, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=K12 / MC4100 / JA176;
RX PubMed=19383799; DOI=10.1073/pnas.0903229106;
RA Malinverni J.C., Silhavy T.J.;
RT "An ABC transport system that maintains lipid asymmetry in the gram-
RT negative outer membrane.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:8009-8014(2009).
CC -!- FUNCTION: Involved in a phospholipid transport pathway that maintains
CC lipid asymmetry in the outer membrane by retrograde trafficking of
CC phospholipids from the outer membrane to the inner membrane.
CC {ECO:0000269|PubMed:19383799}.
CC -!- INTERACTION:
CC P76506; P06996: ompC; NbExp=4; IntAct=EBI-1128511, EBI-371155;
CC P76506; P02931: ompF; NbExp=4; IntAct=EBI-1128511, EBI-371336;
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:19383799}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000305|PubMed:19383799}.
CC -!- DISRUPTION PHENOTYPE: Mutation leads to accumulation of phospholipid in
CC the outer leaflet of the outer membrane and increased outer membrane
CC permeability. It confers sensitivity to SDS-EDTA.
CC {ECO:0000269|PubMed:19383799}.
CC -!- SIMILARITY: Belongs to the MlaA family. {ECO:0000305}.
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DR EMBL; U00096; AAC75406.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16206.2; -; Genomic_DNA.
DR PIR; H65007; H65007.
DR RefSeq; NP_416848.1; NC_000913.3.
DR RefSeq; WP_000776765.1; NZ_LN832404.1.
DR AlphaFoldDB; P76506; -.
DR SMR; P76506; -.
DR BioGRID; 4260538; 183.
DR BioGRID; 849956; 3.
DR IntAct; P76506; 8.
DR STRING; 511145.b2346; -.
DR TCDB; 3.A.1.27.3; the atp-binding cassette (abc) superfamily.
DR jPOST; P76506; -.
DR PaxDb; P76506; -.
DR PRIDE; P76506; -.
DR EnsemblBacteria; AAC75406; AAC75406; b2346.
DR EnsemblBacteria; BAA16206; BAA16206; BAA16206.
DR GeneID; 945582; -.
DR KEGG; ecj:JW2343; -.
DR KEGG; eco:b2346; -.
DR PATRIC; fig|1411691.4.peg.4386; -.
DR EchoBASE; EB4024; -.
DR eggNOG; COG2853; Bacteria.
DR HOGENOM; CLU_059326_3_2_6; -.
DR InParanoid; P76506; -.
DR OMA; PYRYIDH; -.
DR PhylomeDB; P76506; -.
DR BioCyc; EcoCyc:G7216-MON; -.
DR PRO; PR:P76506; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0120010; P:intermembrane phospholipid transfer; IDA:EcoCyc.
DR InterPro; IPR007428; MlaA.
DR PANTHER; PTHR30035; PTHR30035; 1.
DR Pfam; PF04333; MlaA; 1.
DR PRINTS; PR01805; VACJLIPOPROT.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 18..251
FT /note="Intermembrane phospholipid transport system
FT lipoprotein MlaA"
FT /id="PRO_0000018210"
FT REGION 228..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 18
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 18
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CONFLICT 1..4
FT /note="MKLR -> YEAS (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 251 AA; 28042 MW; F008CD42CB8C75B4 CRC64;
MKLRLSALAL GTTLLVGCAS SGTDQQGRSD PLEGFNRTMY NFNFNVLDPY IVRPVAVAWR
DYVPQPARNG LSNFTGNLEE PAVMVNYFLQ GDPYQGMVHF TRFFLNTILG MGGFIDVAGM
ANPKLQRTEP HRFGSTLGHY GVGYGPYVQL PFYGSFTLRD DGGDMADGFY PVLSWLTWPM
SVGKWTLEGI ETRAQLLDSD GLLRQSSDPY IMVREAYFQR HDFIANGGEL KPQENPNAQA
IQDDLKDIDS E
