ID   MLAA_SHIFL              Reviewed;         251 AA.
AC   P43262;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Intermembrane phospholipid transport system lipoprotein MlaA {ECO:0000250|UniProtKB:P76506};
DE   Flags: Precursor;
GN   Name=mlaA {ECO:0000250|UniProtKB:P76506};
GN   Synonyms=vacJ {ECO:0000303|PubMed:8145644};
GN   OrderedLocusNames=SF2424, S2559;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN INTERCELLULAR SPREADING.
RC   STRAIN=YSH6000 / Serotype 2a;
RX   PubMed=8145644; DOI=10.1111/j.1365-2958.1994.tb00287.x;
RA   Suzuki T., Murai T., Fukuda I., Tobe T., Yoshikawa M., Sasakawa C.;
RT   "Identification and characterization of a chromosomal virulence gene, vacJ,
RT   required for intercellular spreading of Shigella flexneri.";
RL   Mol. Microbiol. 11:31-41(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Involved in a phospholipid transport pathway that maintains
CC       lipid asymmetry in the outer membrane by retrograde trafficking of
CC       phospholipids from the outer membrane to the inner membrane (By
CC       similarity). Required for intercellular spreading of S.flexneri
CC       (PubMed:8145644). {ECO:0000250|UniProtKB:P76506,
CC       ECO:0000269|PubMed:8145644}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000250|UniProtKB:P76506}; Lipid-anchor
CC       {ECO:0000250|UniProtKB:P76506, ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the MlaA family. {ECO:0000305}.
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DR   EMBL; D16293; BAA03799.1; -; Genomic_DNA.
DR   EMBL; AE005674; AAN43935.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17748.1; -; Genomic_DNA.
DR   RefSeq; NP_708228.1; NC_004337.2.
DR   RefSeq; WP_000776763.1; NZ_WPGW01000257.1.
DR   AlphaFoldDB; P43262; -.
DR   SMR; P43262; -.
DR   STRING; 198214.SF2424; -.
DR   PRIDE; P43262; -.
DR   EnsemblBacteria; AAN43935; AAN43935; SF2424.
DR   EnsemblBacteria; AAP17748; AAP17748; S2559.
DR   GeneID; 1024405; -.
DR   KEGG; sfl:SF2424; -.
DR   KEGG; sft:NCTC1_02659; -.
DR   KEGG; sfx:S2559; -.
DR   PATRIC; fig|198214.7.peg.2896; -.
DR   HOGENOM; CLU_059326_3_2_6; -.
DR   OMA; PYRYIDH; -.
DR   OrthoDB; 1588560at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007428; MlaA.
DR   PANTHER; PTHR30035; PTHR30035; 1.
DR   Pfam; PF04333; MlaA; 1.
DR   PRINTS; PR01805; VACJLIPOPROT.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal; Virulence.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           18..251
FT                   /note="Intermembrane phospholipid transport system
FT                   lipoprotein MlaA"
FT                   /id="PRO_0000018211"
FT   REGION          228..251
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   251 AA;  28022 MW;  3C6EC026191A3166 CRC64;
     MKLRLSALAL GTTLLVGCAS SGTDQQGRSD PLEGFNRTMY NFNFNVLDPY IVRPVAVAWR
     DYVPQPARNG LSNFTGNLEE PAVMVNYFLQ GDPYQGMVHF TRFFLNTILG MGGFIDVAGM
     ANPKLQRTEP HRFGSTLGHY GVGYGPYVQL PFYGSFTLRD DGGDMADALY PVLSWLTWPM
     SVGKWTLEGI ETRAQLLDSD GLLRQSSDPY IMVREAYFQR HDFIANGGEL KPQENPNAQA
     IQDDLKDIDS E