ID   MLAE_ECOL6              Reviewed;         260 AA.
AC   P64607; P45392;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Intermembrane phospholipid transport system permease protein MlaE {ECO:0000250|UniProtKB:P64606};
GN   Name=mlaE {ECO:0000250|UniProtKB:P64606}; OrderedLocusNames=c3954;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Part of the ABC transporter complex MlaFEDB, which is
CC       involved in a phospholipid transport pathway that maintains lipid
CC       asymmetry in the outer membrane by retrograde trafficking of
CC       phospholipids from the outer membrane to the inner membrane. Probably
CC       responsible for the translocation of the substrate across the membrane.
CC       {ECO:0000250|UniProtKB:P64606}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MlaF),
CC       two transmembrane proteins (MlaE), two cytoplasmic solute-binding
CC       proteins (MlaB) and six periplasmic solute-binding proteins (MlaD).
CC       {ECO:0000250|UniProtKB:P64606}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P64606}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the MlaE permease family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN82394.1; -; Genomic_DNA.
DR   RefSeq; WP_000925795.1; NC_004431.1.
DR   AlphaFoldDB; P64607; -.
DR   SMR; P64607; -.
DR   STRING; 199310.c3954; -.
DR   EnsemblBacteria; AAN82394; AAN82394; c3954.
DR   GeneID; 66672904; -.
DR   KEGG; ecc:c3954; -.
DR   eggNOG; COG0767; Bacteria.
DR   HOGENOM; CLU_045686_1_1_6; -.
DR   OMA; NYLVFPK; -.
DR   BioCyc; ECOL199310:C3954-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   InterPro; IPR003453; ABC_MlaE_Proteobac.
DR   InterPro; IPR030802; Permease_MalE.
DR   PANTHER; PTHR30188; PTHR30188; 1.
DR   Pfam; PF02405; MlaE; 1.
DR   TIGRFAMs; TIGR00056; TIGR00056; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..260
FT                   /note="Intermembrane phospholipid transport system permease
FT                   protein MlaE"
FT                   /id="PRO_0000169467"
FT   TOPO_DOM        1..50
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P64606"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72..88
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P64606"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P64606"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..198
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P64606"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        220..238
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P64606"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        260
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P64606"
SQ   SEQUENCE   260 AA;  27863 MW;  057409EA1E6E25EB CRC64;
     MLLNALASLG HKGIKTLRTF GRAGLMLFNA LVGKPEFRKH APLLVRQLYN VGVLSMLIIV
     VSGVFIGMVL GLQGYLVLTT YSAETSLGML VALSLLRELG PVVAALLFAG RAGSALTAEI
     GLMRATEQLS SMEMMAVDPL RRVISPRFWA GVISLPLLTV IFVAVGIWGG SLVGVSWKGI
     DSGFFWSAMQ NAVDWRMDLV NCLIKSVVFA ITVTWISLFN GYDAIPTSAG ISRATTRTVV
     HSSLAVLGLD FVLTALMFGN