ID   MLC1_MACFA              Reviewed;         377 AA.
AC   Q60HE7;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Membrane protein MLC1;
GN   Name=MLC1; ORFNames=QflA-15415;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Frontal cortex;
RA   Kusuda J., Osada N., Tanuma R., Hirata M., Sugano S., Hashimoto K.;
RT   "Isolation and characterization of cDNA for macaque neurological disease
RT   genes.";
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulates the response of astrocytes to hypo-osmosis by
CC       promoting calcium influx. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ATP1B1. Part of a complex containing ATP1B1,
CC       TRPV4, AQP4 and HEPACAM. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}. Cell membrane {ECO:0000250}. Cytoplasm,
CC       perinuclear region {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB125180; BAD51968.1; -; mRNA.
DR   RefSeq; XP_005567013.1; XM_005566956.2.
DR   RefSeq; XP_015312518.1; XM_015457032.1.
DR   AlphaFoldDB; Q60HE7; -.
DR   STRING; 9541.XP_005567012.1; -.
DR   Ensembl; ENSMFAT00000080566; ENSMFAP00000062932; ENSMFAG00000024753.
DR   GeneID; 102146124; -.
DR   KEGG; mcf:102146124; -.
DR   CTD; 23209; -.
DR   VEuPathDB; HostDB:ENSMFAG00000024753; -.
DR   eggNOG; ENOG502QUF1; Eukaryota.
DR   GeneTree; ENSGT00390000015442; -.
DR   OMA; FSYKTWI; -.
DR   OrthoDB; 856594at2759; -.
DR   Proteomes; UP000233100; Chromosome 10.
DR   Bgee; ENSMFAG00000024753; Expressed in frontal cortex and 2 other tissues.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR   GO; GO:0005901; C:caveola; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
DR   GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; ISS:UniProtKB.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0045121; C:membrane raft; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0055037; C:recycling endosome; ISS:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR   GO; GO:0044877; F:protein-containing complex binding; ISS:UniProtKB.
DR   GO; GO:0072584; P:caveolin-mediated endocytosis; ISS:UniProtKB.
DR   GO; GO:0071397; P:cellular response to cholesterol; ISS:UniProtKB.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0032388; P:positive regulation of intracellular transport; ISS:UniProtKB.
DR   GO; GO:0016192; P:vesicle-mediated transport; ISS:UniProtKB.
DR   InterPro; IPR033280; Membrane_MLC1.
DR   PANTHER; PTHR17597; PTHR17597; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasm; Endoplasmic reticulum; Ion channel;
KW   Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..377
FT                   /note="Membrane protein MLC1"
FT                   /id="PRO_0000096497"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         177
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8VHK5"
FT   MOD_RES         179
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8VHK5"
SQ   SEQUENCE   377 AA;  41158 MW;  936B02128951E1A1 CRC64;
     MTQEPFREEL AYDRMPTLER GRQDPASYAP DTKPSDLQLS KRLPPCFSPK TWVFSVLMGS
     CLLVTSGFSL YLGNVFPAEM DYLRCAAGSC IPSAIVSFTV SRRNANVIPN FQILFVSTFA
     VTTTCLIWFG CKLILNPSAI NINFNLILLL LLELLMAATV IMSARSSEEY CKKKKGSMSD
     GTNILGEVPF PARVLKSYSV VEVIAGISAV LGGIIALNVD DSVSGPHLSV TFFWILVACF
     PSAIASHVTA ECPSKCLVEV LIAISSLTSP LLFTASGYLS FSVMRIVEMF KDYPPAIKPS
     YDVLLLLLLL VLLLQAGLNT GTAIQCVRFK VSARLQGASW DTQSGPQERL AGEVARSPLK
     EFDKEKAWRA VVVQMAQ