MLC1_PROCL
ID MLC1_PROCL Reviewed; 153 AA.
AC J7HCX7;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2012, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=Myosin light chain 1 {ECO:0000303|PubMed:26083097, ECO:0000312|EMBL:AFP95338.1};
DE AltName: Allergen=Pro c 5.0101 {ECO:0000305};
GN Name=MLC1 {ECO:0000303|PubMed:26083097};
OS Procambarus clarkii (Red swamp crayfish).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea;
OC Astacoidea; Cambaridae; Procambarus.
OX NCBI_TaxID=6728 {ECO:0000312|EMBL:AFP95338.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-51, BIOPHYSICOCHEMICAL
RP PROPERTIES, TISSUE SPECIFICITY, GLYCOSYLATION, ALLERGEN, 3D-STRUCTURE
RP MODELING, AND CIRCULAR DICHROISM ANALYSIS.
RX PubMed=26083097; DOI=10.1021/acs.jafc.5b01318;
RA Zhang Y.X., Chen H.L., Maleki S.J., Cao M.J., Zhang L.J., Su W.J.,
RA Liu G.M.;
RT "Purification, Characterization, and Analysis of the Allergenic Properties
RT of Myosin Light Chain in Procambarus clarkii.";
RL J. Agric. Food Chem. 63:6271-6282(2015).
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Stable at acidic (pH 1.0-5.0) and alkaline (pH 9.0 and 11.0)
CC conditions. {ECO:0000269|PubMed:26083097};
CC Temperature dependence:
CC Thermostable even up to 100 degrees Celsius.
CC {ECO:0000269|PubMed:26083097};
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Muscle (at protein level).
CC {ECO:0000269|PubMed:26083097}.
CC -!- PTM: O-glycosylated. {ECO:0000269|PubMed:26083097}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE in all 7
CC patients tested allergic to shrimp. The IgE-binding is not affected in
CC the temperature range of 30 to 100 degrees Celsius, by acidic or
CC alkaline pH or by pepsin digestion, but is reduced by 4-hour digestion
CC with pancreatin. The conformational epitopes rather than the linear
CC epitopes seem to be predominantly recognized by IgE.
CC {ECO:0000269|PubMed:26083097}.
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DR EMBL; JX173714; AFP95338.1; -; mRNA.
DR AlphaFoldDB; J7HCX7; -.
DR SMR; J7HCX7; -.
DR Allergome; 12147; Pro c 5.
DR Allergome; 12148; Pro c 5.0101.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003012; P:muscle system process; IEP:UniProtKB.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR029655; Mlc1.
DR PANTHER; PTHR23048:SF33; PTHR23048:SF33; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Glycoprotein; Motor protein;
KW Muscle protein; Myosin; Repeat.
FT CHAIN 1..153
FT /note="Myosin light chain 1"
FT /id="PRO_0000450597"
FT DOMAIN 8..43
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 82..117
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 135..153
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 48
FT /note="I -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 153 AA; 17591 MW; 35C242E190BE8C6A CRC64;
MAADLSARDV ERVKFAFSIY DFEGNGTMDA FYIGDCLRAL NLNPTLAIIE KVGGKEKRKE
KIIKLEEFIP IFAQVKKDKD AGSYEDFMEV LKLYDKSENG TMMYAELEHI LLSLGERLEK
SELEPVLKEC CNEEDEDGFI PYEPFLKKMT QLL
