MLC5_CAEEL
ID MLC5_CAEEL Reviewed; 142 AA.
AC Q9XVI9;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Myosin-2 essential light chain {ECO:0000303|PubMed:19675126};
GN Name=mlc-5 {ECO:0000312|WormBase:T12D8.6};
GN ORFNames=T12D8.6 {ECO:0000312|WormBase:T12D8.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=19675126; DOI=10.1242/dev.039412;
RA Gally C., Wissler F., Zahreddine H., Quintin S., Landmann F., Labouesse M.;
RT "Myosin II regulation during C. elegans embryonic elongation: LET-502/ROCK,
RT MRCK-1 and PAK-1, three kinases with different roles.";
RL Development 136:3109-3119(2009).
CC -!- FUNCTION: Required for cytokinesis and embryo elongation. May regulate
CC myosin II complex formation and/or the association of myosin with
CC actin. May be involved in the organization of mlc-4 and nmy-2 into
CC bundles. {ECO:0000269|PubMed:19675126}.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains (two
CC regulatory light chains and two essential light chains).
CC {ECO:0000305|PubMed:19675126}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19675126}.
CC Cytoplasm, cytoskeleton {ECO:0000269|PubMed:19675126}. Note=Forms a
CC filamentous structure during 2-fold embryonic stage which may co-
CC localize with actin and whose formation may be regulated by mlc-4.
CC {ECO:0000269|PubMed:19675126}.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis in seam cells as
CC well as in the dorsal and ventral hypodermal cells where it co-
CC localizes with actin bundles. {ECO:0000269|PubMed:19675126}.
CC -!- DISRUPTION PHENOTYPE: Arrest at the 1-cell stage embryo due to a
CC cytokinesis defect characterized by a failure to form a contractile
CC ring. {ECO:0000269|PubMed:19675126}.
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DR EMBL; Z81120; CAB03346.1; -; Genomic_DNA.
DR PIR; T24862; T24862.
DR RefSeq; NP_499813.1; NM_067412.6.
DR AlphaFoldDB; Q9XVI9; -.
DR SMR; Q9XVI9; -.
DR IntAct; Q9XVI9; 1.
DR STRING; 6239.T12D8.6.2; -.
DR EPD; Q9XVI9; -.
DR PaxDb; Q9XVI9; -.
DR PeptideAtlas; Q9XVI9; -.
DR EnsemblMetazoa; T12D8.6.1; T12D8.6.1; WBGene00011734.
DR GeneID; 176796; -.
DR KEGG; cel:CELE_T12D8.6; -.
DR UCSC; T12D8.6.1; c. elegans.
DR CTD; 176796; -.
DR WormBase; T12D8.6; CE16403; WBGene00011734; mlc-5.
DR eggNOG; KOG0030; Eukaryota.
DR GeneTree; ENSGT01030000234570; -.
DR HOGENOM; CLU_061288_2_0_1; -.
DR InParanoid; Q9XVI9; -.
DR OMA; YDRTPKC; -.
DR OrthoDB; 1470794at2759; -.
DR PhylomeDB; Q9XVI9; -.
DR Reactome; R-CEL-5627123; RHO GTPases activate PAKs.
DR PRO; PR:Q9XVI9; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00011734; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:WormBase.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Motor protein; Myosin;
KW Reference proteome; Repeat.
FT CHAIN 1..142
FT /note="Myosin-2 essential light chain"
FT /id="PRO_0000432386"
FT DOMAIN 2..37
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 75..110
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 142 AA; 15971 MW; F3C09D220C797F12 CRC64;
MDDLADCREV FAYFDSKGDE RISVQQVGDV LRALGQNPTE AEIHKCVGSF DREARLSFED
FVPIFQSVSK NREKHTVEEF VEGLSHFDKE GNGMINVAEL RHLLTTLGER LSDEDVDQLL
SGHNDSHGNV NISDFVRAVM NS