MLCB_DICDI
ID MLCB_DICDI Reviewed; 73 AA.
AC Q54GL7;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Myosin-IB light chain;
DE AltName: Full=MyoB light chain;
DE AltName: Full=Myosin light chain mlcB;
DE Short=MlcB;
GN Name=mlcB; ORFNames=DDB_G0290077;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP PROTEIN SEQUENCE OF 34-57, FUNCTION, MUTAGENESIS OF ASP-16, AND SUBUNIT.
RX PubMed=16415352; DOI=10.1074/jbc.m508670200;
RA Crawley S.W., de la Roche M.A., Lee S.F., Li Z., Chitayat S., Smith S.P.,
RA Cote G.P.;
RT "Identification and characterization of an 8-kDa light chain associated
RT with Dictyostelium discoideum MyoB, a class I myosin.";
RL J. Biol. Chem. 281:6307-6315(2006).
CC -!- FUNCTION: Functions as the light chain for myosin-B. Binds calcium with
CC submicromolar affinity and may sense physiological calcium changes.
CC {ECO:0000269|PubMed:16415352}.
CC -!- SUBUNIT: Myosin I is a dimer of a heavy and a light chain. Inability to
CC self-assemble into filaments (By similarity). Interacts with myoB. Does
CC not interact with myoC or myoD. {ECO:0000250,
CC ECO:0000269|PubMed:16415352}.
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DR EMBL; AAFI02000152; EAL62399.1; -; Genomic_DNA.
DR RefSeq; XP_635900.1; XM_630808.1.
DR AlphaFoldDB; Q54GL7; -.
DR BMRB; Q54GL7; -.
DR SMR; Q54GL7; -.
DR STRING; 44689.DDB0233390; -.
DR PaxDb; Q54GL7; -.
DR EnsemblProtists; EAL62399; EAL62399; DDB_G0290077.
DR GeneID; 8627466; -.
DR KEGG; ddi:DDB_G0290077; -.
DR dictyBase; DDB_G0290077; mlcB.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_061288_22_5_1; -.
DR InParanoid; Q54GL7; -.
DR OMA; ILMDQGM; -.
DR PRO; PR:Q54GL7; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0045160; C:myosin I complex; IDA:dictyBase.
DR GO; GO:0005509; F:calcium ion binding; IDA:dictyBase.
DR GO; GO:0017024; F:myosin I binding; IPI:dictyBase.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Metal-binding; Motor protein; Myosin;
KW Reference proteome; Repeat.
FT CHAIN 1..73
FT /note="Myosin-IB light chain"
FT /id="PRO_0000391643"
FT DOMAIN 3..38
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 38..73
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 16
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT BINDING 18
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT BINDING 20
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000305"
FT MUTAGEN 16
FT /note="D->A: Unable to bind Ca(2+)."
FT /evidence="ECO:0000269|PubMed:16415352"
SQ SEQUENCE 73 AA; 8296 MW; C42D88A74B11B93F CRC64;
MSDEKTQLIE AFYNFDGDYD GFVSVEEFRG IIRDGLPMTE AEITEFFEAA DPNNTGFIDY
KAFAAMLYSV DES
