MLE1_CHICK
ID MLE1_CHICK Reviewed; 192 AA.
AC P02604; Q90895; Q9PWQ5;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Myosin light chain 1, skeletal muscle isoform;
DE AltName: Full=Alkali myosin light chain 1;
DE Short=MLC-1;
DE AltName: Full=Myosin light chain 1f;
DE AltName: Full=Myosin light chain alkali 1;
DE Short=Myosin light chain A1;
DE AltName: Full=Skeletal-muscle myosin L-1 light chain;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Skeletal muscle;
RX PubMed=6709041; DOI=10.1038/308333a0;
RA Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.;
RT "Alternative transcription and two modes of splicing results in two myosin
RT light chains from one gene.";
RL Nature 308:333-338(1984).
RN [2]
RP PROTEIN SEQUENCE OF 3-192.
RC TISSUE=Skeletal muscle;
RX PubMed=7238855; DOI=10.1016/0014-5793(81)81045-9;
RA Matsuda G., Maita T., Umegane T.;
RT "The primary structure of L-1 light chain of chicken fast skeletal muscle
RT myosin and its genetic implication.";
RL FEBS Lett. 126:111-113(1981).
RN [3]
RP PROTEIN SEQUENCE OF 3-192.
RC TISSUE=Skeletal muscle;
RX PubMed=7173826; DOI=10.1515/bchm2.1982.363.2.1321;
RA Umegane T., Maita T., Matsuda G.;
RT "Amino-acid sequence of the L-1 light chain of chicken fast skeletal-muscle
RT myosin.";
RL Hoppe-Seyler's Z. Physiol. Chem. 363:1321-1330(1982).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 16-192.
RX PubMed=6128725; DOI=10.1093/nar/10.19.6099;
RA Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.;
RT "Molecular cloning and nucleotide sequences of the complementary DNAs to
RT chicken skeletal muscle myosin two alkali light chain mRNAs.";
RL Nucleic Acids Res. 10:6099-6110(1982).
RN [5]
RP METHYLATION AT ALA-2.
RX PubMed=3979397; DOI=10.1111/j.1432-1033.1985.tb08809.x;
RA Henry G.D., Trayer I.P., Brewer S., Levine B.A.;
RT "The widespread distribution of alpha-N-trimethylalanine as the N-terminal
RT amino acid of light chains from vertebrate striated muscle myosins.";
RL Eur. J. Biochem. 148:75-82(1985).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RX PubMed=8316857; DOI=10.1126/science.8316857;
RA Rayment I., Rypniewski W.R., Schmidt-Base K., Smith R., Tomchick D.R.,
RA Benning M.M., Winkelmann D.A., Wesenberg G., Holden H.M.;
RT "Three-dimensional structure of myosin subfragment-1: a molecular motor.";
RL Science 261:50-58(1993).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=MLC1;
CC IsoId=P02604-1; Sequence=Displayed;
CC Name=MLC3;
CC IsoId=P02605-1; Sequence=External;
CC -!- PTM: The N-terminus is blocked. N,N,N-trimethylalanine, found in other
CC myosin light chains would not have been detected in the N-terminal
CC tryptic peptide in PubMed:6709041 and PubMed:7238855 because it would
CC remain trimethylated and ninhydrin negative after hydrolysis.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; K02610; AAA48955.1; -; Genomic_DNA.
DR EMBL; K02608; AAA48955.1; JOINED; Genomic_DNA.
DR EMBL; K02609; AAA48955.1; JOINED; Genomic_DNA.
DR EMBL; J00887; AAA48960.1; -; mRNA.
DR PIR; A91284; MOCHLA.
DR RefSeq; XP_015144626.1; XM_015289140.1. [P02604-1]
DR RefSeq; XP_015144627.1; XM_015289141.1. [P02604-1]
DR PDB; 1M8Q; EM; 70.00 A; C/F/I/R=51-192.
DR PDB; 2MYS; X-ray; 2.80 A; C=51-192.
DR PDBsum; 1M8Q; -.
DR PDBsum; 2MYS; -.
DR AlphaFoldDB; P02604; -.
DR SMR; P02604; -.
DR STRING; 9031.ENSGALP00000004578; -.
DR Allergome; 11772; Gal d 7.
DR Allergome; 11773; Gal d 7.0101.
DR iPTMnet; P02604; -.
DR PaxDb; P02604; -.
DR Ensembl; ENSGALT00000004587; ENSGALP00000004578; ENSGALG00000002907. [P02604-1]
DR GeneID; 396470; -.
DR CTD; 4632; -.
DR VEuPathDB; HostDB:geneid_396470; -.
DR eggNOG; KOG0030; Eukaryota.
DR GeneTree; ENSGT01030000234570; -.
DR InParanoid; P02604; -.
DR OMA; VAKHDDK; -.
DR OrthoDB; 1470794at2759; -.
DR PhylomeDB; P02604; -.
DR BRENDA; 5.6.1.8; 1306.
DR EvolutionaryTrace; P02604; -.
DR Proteomes; UP000000539; Chromosome 7.
DR Bgee; ENSGALG00000002907; Expressed in muscle tissue and 6 other tissues.
DR ExpressionAtlas; P02604; baseline and differential.
DR GO; GO:0043292; C:contractile fiber; IBA:GO_Central.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0043520; P:regulation of myosin II filament assembly; IDA:CACAO.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Direct protein sequencing; Methylation;
KW Motor protein; Muscle protein; Myosin; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..192
FT /note="Myosin light chain 1, skeletal muscle isoform"
FT /id="PRO_0000198704"
FT DOMAIN 48..83
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 86..121
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 125..160
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 160..192
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..31
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N,N,N-trimethylalanine"
FT /evidence="ECO:0000305|PubMed:3979397"
FT CONFLICT 6
FT /note="D -> E (in Ref. 1; AAA48955)"
FT /evidence="ECO:0000305"
FT CONFLICT 46
FT /note="S -> F (in Ref. 4; AAA48960)"
FT /evidence="ECO:0000305"
FT CONFLICT 116
FT /note="Q -> QM (in Ref. 1; AAA48955)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="Missing (in Ref. 1; AAA48955)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 192 AA; 20899 MW; 6F5594D5C67E63DD CRC64;
MAPKKDVKKP AAAAAPAPAP APAPAPAPAK PKEPAIDLKS IKIEFSKEQQ DDFKEAFLLF
DRTGDAKITL SQVGDIVRAL GQNPTNAEIN KILGNPSKEE MNAKKITFEE FLPMLQAAAN
NKDQGTFEDF VEGLRVFDKE GNGTVMGAEL RHVLATLGEK MTEEEVEELM KGQEDSNGCI
NYEAFVKHIM SV
