MLE3_CHICK
ID MLE3_CHICK Reviewed; 150 AA.
AC P02605; Q90896;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Myosin light chain 3, skeletal muscle isoform;
DE AltName: Full=Alkali myosin light chain 3;
DE Short=MLC-3;
DE AltName: Full=Myosin light chain 3f;
DE AltName: Full=Myosin light chain alkali 2;
DE Short=Myosin light chain A2;
DE AltName: Full=Skeletal-muscle myosin L-4 light chain;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6128725; DOI=10.1093/nar/10.19.6099;
RA Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.;
RT "Molecular cloning and nucleotide sequences of the complementary DNAs to
RT chicken skeletal muscle myosin two alkali light chain mRNAs.";
RL Nucleic Acids Res. 10:6099-6110(1982).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Skeletal muscle;
RX PubMed=6709041; DOI=10.1038/308333a0;
RA Nabeshima Y., Fujii-Kuriyama Y., Muramatsu M., Ogata K.;
RT "Alternative transcription and two modes of splicing results in two myosin
RT light chains from one gene.";
RL Nature 308:333-338(1984).
RN [3]
RP PROTEIN SEQUENCE OF 2-150, AND BLOCKAGE OF N-TERMINUS.
RC TISSUE=Skeletal muscle;
RX PubMed=6783403; DOI=10.1111/j.1432-1033.1981.tb06169.x;
RA Maita T., Umegane T., Matsuda G.;
RT "Amino-acid sequence of the L-4 light chain of chicken skeletal-muscle
RT myosin.";
RL Eur. J. Biochem. 114:45-49(1981).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=MLC3;
CC IsoId=P02605-1; Sequence=Displayed;
CC Name=MLC1;
CC IsoId=P02604-1; Sequence=External;
CC -!- PTM: The N-terminus is blocked.
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DR EMBL; J00888; AAA48961.1; -; mRNA.
DR EMBL; K02610; AAA48956.1; -; Genomic_DNA.
DR EMBL; K02609; AAA48956.1; JOINED; Genomic_DNA.
DR PIR; I50384; MOCHA2.
DR RefSeq; NP_001038097.1; NM_001044632.1.
DR PDB; 1MVW; EM; 70.00 A; C/F/I/L/O/R=10-150.
DR PDB; 1O18; EM; 70.00 A; F/I/L/O/R=10-150.
DR PDB; 1O19; EM; 70.00 A; C/F/I/L/O/U=10-150.
DR PDB; 1O1A; EM; 70.00 A; C/F/I/L/O/R=10-150.
DR PDB; 1O1B; EM; 70.00 A; C/F/I/L=10-150.
DR PDB; 1O1C; EM; 70.00 A; C/F/I/L/R=10-150.
DR PDB; 1O1D; EM; 70.00 A; C/F/I/L/O/R=10-150.
DR PDB; 1O1E; EM; 70.00 A; C/F/I/L/O/R=10-150.
DR PDB; 1O1F; EM; 70.00 A; C/F/I/L=10-150.
DR PDB; 1O1G; EM; 70.00 A; C/F/I/L/O/R=10-150.
DR PDB; 2W4A; EM; 35.00 A; C=6-150.
DR PDB; 2W4G; EM; 35.00 A; C=6-150.
DR PDB; 2W4H; EM; 35.00 A; C=6-150.
DR PDBsum; 1MVW; -.
DR PDBsum; 1O18; -.
DR PDBsum; 1O19; -.
DR PDBsum; 1O1A; -.
DR PDBsum; 1O1B; -.
DR PDBsum; 1O1C; -.
DR PDBsum; 1O1D; -.
DR PDBsum; 1O1E; -.
DR PDBsum; 1O1F; -.
DR PDBsum; 1O1G; -.
DR PDBsum; 2W4A; -.
DR PDBsum; 2W4G; -.
DR PDBsum; 2W4H; -.
DR AlphaFoldDB; P02605; -.
DR SMR; P02605; -.
DR Ensembl; ENSGALT00000040054; ENSGALP00000039262; ENSGALG00000002907. [P02605-1]
DR GeneID; 396470; -.
DR KEGG; gga:396470; -.
DR CTD; 4632; -.
DR VEuPathDB; HostDB:geneid_396470; -.
DR GeneTree; ENSGT01030000234570; -.
DR HOGENOM; CLU_061288_13_0_1; -.
DR EvolutionaryTrace; P02605; -.
DR Proteomes; UP000000539; Chromosome 7.
DR Bgee; ENSGALG00000002907; Expressed in muscle tissue and 6 other tissues.
DR ExpressionAtlas; P02605; baseline and differential.
DR GO; GO:0043292; C:contractile fiber; IBA:GO_Central.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Direct protein sequencing;
KW Motor protein; Muscle protein; Myosin; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:6783403"
FT CHAIN 2..150
FT /note="Myosin light chain 3, skeletal muscle isoform"
FT /id="PRO_0000198705"
FT DOMAIN 6..41
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 44..79
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 83..118
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 118..150
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 2
FT /note="Blocked amino end (Ser)"
FT CONFLICT 7..9
FT /note="EIN -> QID (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 74
FT /note="Q -> QM (in Ref. 2; AAA48956)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="Missing (in Ref. 2; AAA48956)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="M -> I (in Ref. 2; AAA48961)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 150 AA; 16710 MW; 6F7BE6BA70055BE0 CRC64;
MSFSPDEIND FKEAFLLFDR TGDAKITLSQ VGDIVRALGQ NPTNAEINKI LGNPSKEEMN
AKKITFEEFL PMLQAAANNK DQGTFEDFVE GLRVFDKEGN GTVMGAELRH VLATLGEKMT
EEEVEELMKG QEDSNGCINY EAFVKHIMSV
