ID   MLEN_BACSU              Reviewed;         468 AA.
AC   P54571;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Malate-2H(+)/Na(+)-lactate antiporter;
GN   Name=mleN; Synonyms=yqkI; OrderedLocusNames=BSU23560;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=10903309; DOI=10.1074/jbc.m001112200;
RA   Wei Y., Guffanti A.A., Ito M., Krulwich T.A.;
RT   "Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that
RT   enhances growth on malate at low protonmotive force.";
RL   J. Biol. Chem. 275:30287-30292(2000).
CC   -!- FUNCTION: Couples proton uptake and Na(+) efflux to the substrate-
CC       product malate/lactate antiport, in an electroneutral malate-
CC       2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high
CC       density on malate at reduced protonmotive force.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the NhaC Na(+)/H(+) (TC 2.A.35) antiporter
CC       family. {ECO:0000305}.
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DR   EMBL; D84432; BAA12644.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14288.1; -; Genomic_DNA.
DR   PIR; B69967; B69967.
DR   RefSeq; NP_390237.1; NC_000964.3.
DR   RefSeq; WP_003230432.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P54571; -.
DR   STRING; 224308.BSU23560; -.
DR   TCDB; 2.A.35.1.2; the nhac na(+):h(+) antiporter (nhac) family.
DR   PaxDb; P54571; -.
DR   PRIDE; P54571; -.
DR   EnsemblBacteria; CAB14288; CAB14288; BSU_23560.
DR   GeneID; 938720; -.
DR   KEGG; bsu:BSU23560; -.
DR   PATRIC; fig|224308.179.peg.2568; -.
DR   eggNOG; COG1757; Bacteria.
DR   InParanoid; P54571; -.
DR   OMA; LGNIFGC; -.
DR   PhylomeDB; P54571; -.
DR   BioCyc; BSUB:BSU23560-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; IDA:CACAO.
DR   InterPro; IPR004770; Na/H_antiport_NhaC.
DR   InterPro; IPR018461; Na/H_Antiport_NhaC-like_C.
DR   Pfam; PF03553; Na_H_antiporter; 1.
DR   TIGRFAMs; TIGR00931; antiport_nhaC; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell membrane; Ion transport; Membrane; Reference proteome;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..468
FT                   /note="Malate-2H(+)/Na(+)-lactate antiporter"
FT                   /id="PRO_0000052417"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        192..212
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        233..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        428..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   468 AA;  50212 MW;  5C312F4ECDBBB4AE CRC64;
     MKDVRLPTLF EIIIVLGVFL ALVLSFTVFL DLPIQLALFV SWFIAMLLGI RLGYSYKDLQ
     NAIVHGISNG LEAVLILVSV GALIGTWIAG GVVPTLIYYG LEFIHPSIFL LATLIICSIM
     SVATGTSWGT VGTAGIAMIA IGEGLGIPLP LVAGAILSGA YFGDKLSPLS DSTVLASSLS
     KVDVLAHVRA MLYLSIPAYV ITAILFTVVG FMYGGKNIDL DKVEFLKSSL QNTFDIHIWM
     LIPAVLVIVL LAMKKPSMPV IVIGALLGAI WAVVFQGMDI AHAIATAYNG FSIKTDVEFL
     NGLLNRGGIV GMLDSLVVII FGLGFGGLLE KLGVLKVIVS TFEKKLTSAG NVTLSTLIVA
     FLANIFGCAM YVSLILTPKI MEDSYDRLHL DRRVLSRNSE VGGTLTSGMV PWSDNGIYMA
     GILGVSTFSY LPFMWLSFVA IGLAIIYGYT GKFIWYTKNN TVKAEKLG