ID   MLER_LACLA              Reviewed;         291 AA.
AC   P16400;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=Malolactic fermentation system transcriptional activator;
GN   Name=mleR; OrderedLocusNames=LL0872; ORFNames=L0148;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2498286; DOI=10.1128/jb.171.6.3108-3114.1989;
RA   Renault P., Gaillardin C., Heslot H.;
RT   "Product of the Lactococcus lactis gene required for malolactic
RT   fermentation is homologous to a family of positive regulators.";
RL   J. Bacteriol. 171:3108-3114(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Required for malolactic fermentation. It is most probably a
CC       transcriptional activator.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; M90762; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AE005176; AAK04970.1; -; Genomic_DNA.
DR   PIR; A44510; A44510.
DR   PIR; H86733; H86733.
DR   RefSeq; NP_267028.1; NC_002662.1.
DR   RefSeq; WP_010905602.1; NC_002662.1.
DR   AlphaFoldDB; P16400; -.
DR   SMR; P16400; -.
DR   STRING; 272623.L0148; -.
DR   PaxDb; P16400; -.
DR   DNASU; 1114502; -.
DR   EnsemblBacteria; AAK04970; AAK04970; L0148.
DR   KEGG; lla:L0148; -.
DR   PATRIC; fig|272623.7.peg.933; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_6_2_9; -.
DR   OMA; EPYIMLT; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..291
FT                   /note="Malolactic fermentation system transcriptional
FT                   activator"
FT                   /id="PRO_0000105682"
FT   DOMAIN          1..60
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        20..39
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   291 AA;  33965 MW;  0B2C250FD332C7CC CRC64;
     MSLNLRDLEY FYQLSKLRSF TNVAKHFRVS QPTISYAIKR LETYYDCDLF YKDSSHQVVD
     LTPEGELLAM RTNEILSELN HTKKAIHRSS MKDFKVGFPP IISSYLLSKQ DNLNFFNALH
     MIYGGSNELL SLLLNEEIDS SLLGSLEKIK HPQLEIEELF QKEFFIIMSD SHPLANEKEL
     GFKDLLNEDF ILLGEHNIHF NAFNRLNEKY NQSANVVLKL DDAHTIKELV RKNLGISLMA
     DIRLSEDFKN LVKVPFIEED KQFFHINYAY QKNSILSESE KNFLEILKTL K