MLF2_HUMAN
ID MLF2_HUMAN Reviewed; 248 AA.
AC Q15773;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Myeloid leukemia factor 2;
DE AltName: Full=Myelodysplasia-myeloid leukemia factor 2;
GN Name=MLF2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8661158; DOI=10.1006/geno.1996.0376;
RA Kuefer M.U., Look A.T., Williams D.C., Valentine V., Naeve C.W., Behm F.G.,
RA Mullersman J.E., Yoneda-Kato N., Montgomery K., Kucherlapati R.,
RA Morris S.W.;
RT "cDNA cloning, tissue distribution, and chromosomal localization of
RT myelodysplasia/myeloid leukemia factor 2 (MLF2).";
RL Genomics 35:392-396(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RA Yu W., Gibbs R.A.;
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT networks.";
RL Cell 127:635-648(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19369195; DOI=10.1074/mcp.m800588-mcp200;
RA Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,
RA Mann M., Daub H.;
RT "Large-scale proteomics analysis of the human kinome.";
RL Mol. Cell. Proteomics 8:1751-1764(2009).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238 AND SER-240, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238 AND SER-240, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [14]
RP VARIANT [LARGE SCALE ANALYSIS] CYS-80.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- INTERACTION:
CC Q15773; Q99933: BAG1; NbExp=2; IntAct=EBI-1051875, EBI-1030678;
CC Q15773; O95816: BAG2; NbExp=4; IntAct=EBI-1051875, EBI-355275;
CC Q15773; O95817: BAG3; NbExp=4; IntAct=EBI-1051875, EBI-747185;
CC Q15773; O75190: DNAJB6; NbExp=4; IntAct=EBI-1051875, EBI-1053164;
CC Q15773; Q9UJY1: HSPB8; NbExp=3; IntAct=EBI-1051875, EBI-739074;
CC Q15773; P58340: MLF1; NbExp=2; IntAct=EBI-1051875, EBI-721328;
CC Q15773; Q9UNE7: STUB1; NbExp=3; IntAct=EBI-1051875, EBI-357085;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC -!- SIMILARITY: Belongs to the MLF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U57342; AAB08988.1; -; mRNA.
DR EMBL; AF070539; AAC28632.1; -; mRNA.
DR EMBL; BC000898; AAH00898.1; -; mRNA.
DR EMBL; BC002340; AAH02340.1; -; mRNA.
DR CCDS; CCDS8559.1; -.
DR RefSeq; NP_005430.1; NM_005439.2.
DR AlphaFoldDB; Q15773; -.
DR BioGRID; 113752; 138.
DR IntAct; Q15773; 133.
DR MINT; Q15773; -.
DR STRING; 9606.ENSP00000203630; -.
DR ChEMBL; CHEMBL4295830; -.
DR MoonDB; Q15773; Predicted.
DR iPTMnet; Q15773; -.
DR PhosphoSitePlus; Q15773; -.
DR BioMuta; MLF2; -.
DR DMDM; 17368482; -.
DR EPD; Q15773; -.
DR jPOST; Q15773; -.
DR MassIVE; Q15773; -.
DR PaxDb; Q15773; -.
DR PeptideAtlas; Q15773; -.
DR PRIDE; Q15773; -.
DR ProteomicsDB; 60755; -.
DR Antibodypedia; 2268; 250 antibodies from 28 providers.
DR DNASU; 8079; -.
DR Ensembl; ENST00000203630.10; ENSP00000203630.5; ENSG00000089693.11.
DR Ensembl; ENST00000435120.5; ENSP00000403941.1; ENSG00000089693.11.
DR Ensembl; ENST00000539187.5; ENSP00000446317.1; ENSG00000089693.11.
DR GeneID; 8079; -.
DR KEGG; hsa:8079; -.
DR MANE-Select; ENST00000203630.10; ENSP00000203630.5; NM_001382226.1; NP_001369155.1.
DR CTD; 8079; -.
DR DisGeNET; 8079; -.
DR GeneCards; MLF2; -.
DR HGNC; HGNC:7126; MLF2.
DR HPA; ENSG00000089693; Low tissue specificity.
DR MIM; 601401; gene.
DR neXtProt; NX_Q15773; -.
DR OpenTargets; ENSG00000089693; -.
DR PharmGKB; PA30844; -.
DR VEuPathDB; HostDB:ENSG00000089693; -.
DR eggNOG; KOG4049; Eukaryota.
DR GeneTree; ENSGT00390000005023; -.
DR HOGENOM; CLU_063313_3_0_1; -.
DR InParanoid; Q15773; -.
DR OMA; DDPFMMD; -.
DR OrthoDB; 1471165at2759; -.
DR PhylomeDB; Q15773; -.
DR TreeFam; TF317561; -.
DR PathwayCommons; Q15773; -.
DR SignaLink; Q15773; -.
DR BioGRID-ORCS; 8079; 16 hits in 1069 CRISPR screens.
DR ChiTaRS; MLF2; human.
DR GenomeRNAi; 8079; -.
DR Pharos; Q15773; Tbio.
DR PRO; PR:Q15773; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q15773; protein.
DR Bgee; ENSG00000089693; Expressed in right frontal lobe and 211 other tissues.
DR ExpressionAtlas; Q15773; baseline and differential.
DR Genevisible; Q15773; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR019376; Myeloid_leukemia_factor.
DR PANTHER; PTHR13105; PTHR13105; 1.
DR Pfam; PF10248; Mlf1IP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..248
FT /note="Myeloid leukemia factor 2"
FT /id="PRO_0000220754"
FT REGION 130..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 217
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 238
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 240
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT VARIANT 80
FT /note="F -> C (in a colorectal cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036429"
SQ SEQUENCE 248 AA; 28147 MW; EB1DC52AD1767715 CRC64;
MFRFMRDVEP EDPMFLMDPF AIHRQHMSRM LSGGFGYSPF LSITDGNMPG TRPASRRMQQ
AGAVSPFGML GMSGGFMDMF GMMNDMIGNM EHMTAGGNCQ TFSSSTVISY SNTGDGAPKV
YQETSEMRSA PGGIRETRRT VRDSDSGLEQ MSIGHHIRDR AHILQRSRNH RTGDQEERQD
YINLDESEAA AFDDEWRRET SRFRQQRPLE FRRLESSGAG GRRAEGPPRL AIQGPEDSPS
RQSRRYDW
