MLF_DROME
ID MLF_DROME Reviewed; 376 AA.
AC Q9NKV0; A1ZA61; A1ZA62; Q540Y3; Q8IG90; Q95P43; Q9V7G3;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Myeloid leukemia factor;
DE AltName: Full=Myelodysplasia-myeloid leukemia factor;
DE Short=dMLF;
GN Name=Mlf; ORFNames=CG8295;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), INTERACTION WITH DREF, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11137299; DOI=10.1016/s0378-1119(00)00447-9;
RA Ohno K., Takahashi Y., Hirose F., Inoue Y.H., Taguchi O., Nishida Y.,
RA Matsukage A., Yamaguchi M.;
RT "Characterization of a Drosophila homologue of the human
RT myelodysplasia/myeloid leukemia factor (MLF).";
RL Gene 260:133-143(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RC TISSUE=Head;
RX PubMed=12354791; DOI=10.1093/hmg/11.21.2657;
RA Kazemi-Esfarjani P., Benzer S.;
RT "Suppression of polyglutamine toxicity by a Drosophila homolog of myeloid
RT leukemia factor 1.";
RL Hum. Mol. Genet. 11:2657-2672(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-323, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- SUBUNIT: Interacts with DRE-binding factor Dref.
CC {ECO:0000269|PubMed:11137299}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11137299}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=D;
CC IsoId=Q9NKV0-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q9NKV0-2; Sequence=VSP_026176;
CC Name=B;
CC IsoId=Q9NKV0-3; Sequence=VSP_026175, VSP_026177;
CC Name=C;
CC IsoId=Q9NKV0-4; Sequence=VSP_026174;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in unfertilized eggs,
CC early embryos, pupae and adult males while a low level expression is
CC found in adult females and larvae. {ECO:0000269|PubMed:11137299}.
CC -!- DEVELOPMENTAL STAGE: High levels are seen in unfertilized eggs and
CC expression increases slightly during early embryo stages (2-3 hours).
CC Levels are high in embryos until 4 hours after fertilization and then
CC decrease gradually through embryonic and larval stages.
CC {ECO:0000269|PubMed:11137299}.
CC -!- SIMILARITY: Belongs to the MLF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB043986; BAA96391.1; -; mRNA.
DR EMBL; AY037049; AAK63191.1; -; mRNA.
DR EMBL; AE013599; AAF58093.2; -; Genomic_DNA.
DR EMBL; AE013599; AAS64849.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM70968.2; -; Genomic_DNA.
DR EMBL; AE013599; AAM70969.1; -; Genomic_DNA.
DR EMBL; AY118583; AAM49952.1; -; mRNA.
DR EMBL; BT001895; AAN71684.1; -; mRNA.
DR RefSeq; NP_523753.1; NM_079029.5. [Q9NKV0-2]
DR RefSeq; NP_725501.2; NM_166122.4. [Q9NKV0-4]
DR RefSeq; NP_725502.1; NM_166123.4. [Q9NKV0-3]
DR RefSeq; NP_995847.1; NM_206125.2. [Q9NKV0-1]
DR AlphaFoldDB; Q9NKV0; -.
DR BioGRID; 62477; 13.
DR IntAct; Q9NKV0; 1.
DR STRING; 7227.FBpp0089346; -.
DR iPTMnet; Q9NKV0; -.
DR PaxDb; Q9NKV0; -.
DR PRIDE; Q9NKV0; -.
DR DNASU; 36750; -.
DR EnsemblMetazoa; FBtr0087292; FBpp0086428; FBgn0034051. [Q9NKV0-2]
DR EnsemblMetazoa; FBtr0087293; FBpp0086429; FBgn0034051. [Q9NKV0-3]
DR EnsemblMetazoa; FBtr0087294; FBpp0086430; FBgn0034051. [Q9NKV0-4]
DR EnsemblMetazoa; FBtr0087295; FBpp0089346; FBgn0034051. [Q9NKV0-1]
DR GeneID; 36750; -.
DR KEGG; dme:Dmel_CG8295; -.
DR CTD; 36750; -.
DR FlyBase; FBgn0034051; Mlf.
DR VEuPathDB; VectorBase:FBgn0034051; -.
DR eggNOG; KOG4049; Eukaryota.
DR GeneTree; ENSGT00390000005023; -.
DR InParanoid; Q9NKV0; -.
DR OMA; MGAMAPM; -.
DR PhylomeDB; Q9NKV0; -.
DR BioGRID-ORCS; 36750; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Mlf; fly.
DR GenomeRNAi; 36750; -.
DR PRO; PR:Q9NKV0; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034051; Expressed in brain and 39 other tissues.
DR ExpressionAtlas; Q9NKV0; baseline and differential.
DR Genevisible; Q9NKV0; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR GO; GO:0005705; C:polytene chromosome interband; IDA:FlyBase.
DR GO; GO:0051087; F:chaperone binding; IPI:FlyBase.
DR GO; GO:0008134; F:transcription factor binding; IPI:FlyBase.
DR GO; GO:0042691; P:positive regulation of crystal cell differentiation; IMP:FlyBase.
DR GO; GO:0050821; P:protein stabilization; IMP:FlyBase.
DR GO; GO:2000495; P:regulation of cell proliferation involved in compound eye morphogenesis; IEP:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:FlyBase.
DR InterPro; IPR019376; Myeloid_leukemia_factor.
DR PANTHER; PTHR13105; PTHR13105; 1.
DR Pfam; PF10248; Mlf1IP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..376
FT /note="Myeloid leukemia factor"
FT /id="PRO_0000220756"
FT REGION 96..202
FT /note="Interaction with DREF"
FT /evidence="ECO:0000269|PubMed:11137299"
FT REGION 124..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 187..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..307
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 326..340
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..369
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 323
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 2..19
FT /note="SLFGALMGDFDDDLGLMN -> KQKRKSKSNRSQMELH (in isoform
FT C)"
FT /evidence="ECO:0000305"
FT /id="VSP_026174"
FT VAR_SEQ 270..273
FT /note="SGNI -> RYNY (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12354791"
FT /id="VSP_026175"
FT VAR_SEQ 271..337
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|PubMed:11137299,
FT ECO:0000303|PubMed:12537569"
FT /id="VSP_026176"
FT VAR_SEQ 274..376
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12354791"
FT /id="VSP_026177"
FT CONFLICT 139
FT /note="R -> K (in Ref. 5; AAN71684)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="S -> T (in Ref. 5; AAN71684)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="F -> Y (in Ref. 5; AAN71684)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 41377 MW; AD61C1CBD15AD0D0 CRC64;
MSLFGALMGD FDDDLGLMNN HMNHTMNAMN MQMRSMNRLM NSFMPDPFMQ VSPFDQGFQQ
NALMERPQMP AMPAMGLFGM PMMPNFNRLL NADIGGNSGA SFCQSTVMTM SSGPDGRPQI
YQASTSTKTG PGGVRETRRT VQDSRTGVKK MAIGHHIGER AHIIEKEQDM RSGQLEERQE
FINLEEGEAE QFDREFTSRA SRGAVQSRHH AGGMQAIMPA RPAAHTSTLT IEPVEDDDDD
DDDCVIQEQQ PVRSSAGRHY SSAPTAPQNS GNIATAAAPT PTSSPTTYDT SNGNNNNYVS
SRRSYLRNGH GHSLATPRRP LRTPPSSPLA TVSTSPSIHP HPYAANPRRQ QRAVKHFHTE
DEAASSYKAV KRGKKK
