ID   MLIC_CUPNH              Reviewed;         125 AA.
AC   Q0K8S4;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Membrane-bound lysozyme inhibitor of C-type lysozyme {ECO:0000250|UniProtKB:Q9I574};
DE   Flags: Precursor;
GN   Name=mliC; OrderedLocusNames=H16_A2509;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: Specifically inhibits C-type lysozymes.
CC       {ECO:0000250|UniProtKB:Q9I574}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q9I574}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000250|UniProtKB:P28224}; Lipid-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}. Note=Anchored to the periplasmic side.
CC       {ECO:0000250|UniProtKB:P28224}.
CC   -!- SIMILARITY: Belongs to the MliC family. Type 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AM260479; CAJ93597.1; -; Genomic_DNA.
DR   RefSeq; WP_011615702.1; NZ_CP039287.1.
DR   AlphaFoldDB; Q0K8S4; -.
DR   SMR; Q0K8S4; -.
DR   STRING; 381666.H16_A2509; -.
DR   EnsemblBacteria; CAJ93597; CAJ93597; H16_A2509.
DR   GeneID; 57644635; -.
DR   KEGG; reh:H16_A2509; -.
DR   PATRIC; fig|381666.6.peg.2901; -.
DR   eggNOG; COG3895; Bacteria.
DR   HOGENOM; CLU_151151_0_0_4; -.
DR   OMA; TWWTKGP; -.
DR   OrthoDB; 1987224at2; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.128.200; -; 1.
DR   InterPro; IPR018660; MliC.
DR   InterPro; IPR036328; MliC_sf.
DR   Pfam; PF09864; MliC; 1.
DR   SUPFAM; SSF141488; SSF141488; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Disulfide bond; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           22..125
FT                   /note="Membrane-bound lysozyme inhibitor of C-type
FT                   lysozyme"
FT                   /id="PRO_5000079991"
FT   SITE            85
FT                   /note="Directly involved in lysozyme active site
FT                   inhibition"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I574"
FT   SITE            99
FT                   /note="Directly involved in lysozyme active site
FT                   inhibition"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I574"
FT   LIPID           22
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           22
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   DISULFID        48..121
FT                   /evidence="ECO:0000250|UniProtKB:Q9I574"
SQ   SEQUENCE   125 AA;  13197 MW;  A765E10361CD34F4 CRC64;
     MTTRPILSCA RAVALLATLG ACAAAHAARA PGIDGVRFPD VRAVRYQCDG GKTLSVRYFN
     SPDNQAAVFR IDGKPVLAVS TVSASGARYA GGRYEWWTKG EEGTLRDLMQ AENAAPALAN
     CQAKP