MLO2_ARATH
ID MLO2_ARATH Reviewed; 573 AA.
AC Q9SXB6;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=MLO-like protein 2;
DE Short=AtMlo2;
GN Name=MLO2; OrderedLocusNames=At1g11310; ORFNames=T28P6.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12569425; DOI=10.1007/s00239-002-2382-5;
RA Devoto A., Hartmann H.A., Piffanelli P., Elliott C., Simmons C.,
RA Taramino G., Goh C.-S., Cohen F.E., Emerson B.C., Schulze-Lefert P.,
RA Panstruga R.;
RT "Molecular phylogeny and evolution of the plant-specific seven-
RT transmembrane MLO family.";
RL J. Mol. Evol. 56:77-88(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH CALMODULIN.
RX PubMed=11919636; DOI=10.1038/416447a;
RA Kim M.C., Panstruga R., Elliott C., Mueller J., Devoto A., Yoon H.W.,
RA Park H.C., Cho M.J., Schulze-Lefert P.;
RT "Calmodulin interacts with MLO protein to regulate defence against mildew
RT in barley.";
RL Nature 416:447-451(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: May be involved in modulation of pathogen defense and leaf
CC cell death. Activity seems to be regulated by Ca(2+)-dependent
CC calmodulin binding and seems not to require heterotrimeric G proteins
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SXB6-1; Sequence=Displayed;
CC -!- DOMAIN: The C-terminus contains a calmodulin-binding domain, which
CC binds calmodulin in a calcium-dependent fashion.
CC -!- SIMILARITY: Belongs to the MLO family. {ECO:0000305}.
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DR EMBL; AF369563; AAK53795.1; -; mRNA.
DR EMBL; AC007259; AAD49991.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28715.1; -; Genomic_DNA.
DR EMBL; AY086586; AAM63648.1; -; mRNA.
DR PIR; B86247; B86247.
DR RefSeq; NP_172598.1; NM_101004.4. [Q9SXB6-1]
DR AlphaFoldDB; Q9SXB6; -.
DR SMR; Q9SXB6; -.
DR BioGRID; 22913; 13.
DR IntAct; Q9SXB6; 2.
DR STRING; 3702.AT1G11310.1; -.
DR iPTMnet; Q9SXB6; -.
DR PaxDb; Q9SXB6; -.
DR PRIDE; Q9SXB6; -.
DR ProteomicsDB; 251413; -. [Q9SXB6-1]
DR EnsemblPlants; AT1G11310.1; AT1G11310.1; AT1G11310. [Q9SXB6-1]
DR GeneID; 837673; -.
DR Gramene; AT1G11310.1; AT1G11310.1; AT1G11310. [Q9SXB6-1]
DR KEGG; ath:AT1G11310; -.
DR Araport; AT1G11310; -.
DR TAIR; locus:2202064; AT1G11310.
DR eggNOG; ENOG502QVKX; Eukaryota.
DR InParanoid; Q9SXB6; -.
DR PhylomeDB; Q9SXB6; -.
DR PRO; PR:Q9SXB6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SXB6; baseline and differential.
DR Genevisible; Q9SXB6; AT.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0031348; P:negative regulation of defense response; IMP:TAIR.
DR GO; GO:0009620; P:response to fungus; IMP:TAIR.
DR InterPro; IPR004326; Mlo.
DR PANTHER; PTHR31942; PTHR31942; 1.
DR Pfam; PF03094; Mlo; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calmodulin-binding; Membrane;
KW Pathogenesis-related protein; Phosphoprotein; Plant defense;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..573
FT /note="MLO-like protein 2"
FT /id="PRO_0000209932"
FT TOPO_DOM 1..15
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..164
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..287
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..317
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..415
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 437..573
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 450..471
FT /note="Calmodulin-binding"
FT REGION 462..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 472..513
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 516..543
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 512
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 573 AA; 65544 MW; 36FA911F8B1D94A6 CRC64;
MADQVKERTL EETSTWAVAV VCFVLLFISI VLEHSIHKIG TWFKKKHKQA LFEALEKVKA
ELMLLGFISL LLTIGQTPIS NICISQKVAS TMHPCSAAEE AKKYGKKDAG KKDDGDGDKP
GRRLLLELAE SYIHRRSLAT KGYDKCAEKG KVAFVSAYGI HQLHIFIFVL AVVHVVYCIV
TYAFGKIKMR TWKSWEEETK TIEYQYSNDP ERFRFARDTS FGRRHLNFWS KTRVTLWIVC
FFRQFFGSVT KVDYLALRHG FIMAHFAPGN ESRFDFRKYI QRSLEKDFKT VVEISPVIWF
VAVLFLLTNS YGLRSYLWLP FIPLVVILIV GTKLEVIITK LGLRIQEKGD VVRGAPVVQP
GDDLFWFGKP RFILFLIHLV LFTNAFQLAF FAWSTYEFNL NNCFHESTAD VVIRLVVGAV
VQILCSYVTL PLYALVTQMG SKMKPTVFND RVATALKKWH HTAKNETKHG RHSGSNTPFS
SRPTTPTHGS SPIHLLHNFN NRSVENYPSS PSPRYSGHGH HEHQFWDPES QHQEAETSTH
HSLAHESSEP VLASVELPPI RTSKSLRDFS FKK
