ID   MLO5_ARATH              Reviewed;         501 AA.
AC   O22815;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=MLO-like protein 5;
DE            Short=AtMlo5;
GN   Name=MLO5; OrderedLocusNames=At2g33670; ORFNames=F4P9.44, T1B8.26;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12569425; DOI=10.1007/s00239-002-2382-5;
RA   Devoto A., Hartmann H.A., Piffanelli P., Elliott C., Simmons C.,
RA   Taramino G., Goh C.-S., Cohen F.E., Emerson B.C., Schulze-Lefert P.,
RA   Panstruga R.;
RT   "Molecular phylogeny and evolution of the plant-specific seven-
RT   transmembrane MLO family.";
RL   J. Mol. Evol. 56:77-88(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: May be involved in modulation of pathogen defense and leaf
CC       cell death. Activity seems to be regulated by Ca(2+)-dependent
CC       calmodulin binding and seems not to require heterotrimeric G proteins
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- DOMAIN: The C-terminus contains a calmodulin-binding domain, which
CC       binds calmodulin in a calcium-dependent fashion. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MLO family. {ECO:0000305}.
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DR   EMBL; AF369566; AAK53798.1; -; mRNA.
DR   EMBL; AC002332; AAM14803.1; -; Genomic_DNA.
DR   EMBL; U78721; AAC69142.3; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08867.1; -; Genomic_DNA.
DR   PIR; B84748; B84748.
DR   RefSeq; NP_180923.1; NM_128925.2.
DR   AlphaFoldDB; O22815; -.
DR   BioGRID; 3279; 13.
DR   IntAct; O22815; 13.
DR   STRING; 3702.AT2G33670.1; -.
DR   TCDB; 9.B.197.1.2; the mildew resistance locus o (mlo) family.
DR   PaxDb; O22815; -.
DR   PRIDE; O22815; -.
DR   ProteomicsDB; 238712; -.
DR   EnsemblPlants; AT2G33670.1; AT2G33670.1; AT2G33670.
DR   GeneID; 817932; -.
DR   Gramene; AT2G33670.1; AT2G33670.1; AT2G33670.
DR   KEGG; ath:AT2G33670; -.
DR   Araport; AT2G33670; -.
DR   TAIR; locus:2051073; AT2G33670.
DR   eggNOG; ENOG502QPZ5; Eukaryota.
DR   HOGENOM; CLU_024720_1_0_1; -.
DR   InParanoid; O22815; -.
DR   OMA; EWEKETC; -.
DR   OrthoDB; 603362at2759; -.
DR   PhylomeDB; O22815; -.
DR   PRO; PR:O22815; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O22815; baseline and differential.
DR   Genevisible; O22815; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   GO; GO:0043621; F:protein self-association; IPI:TAIR.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0010183; P:pollen tube guidance; IGI:TAIR.
DR   GO; GO:0072659; P:protein localization to plasma membrane; IGI:TAIR.
DR   GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR   InterPro; IPR004326; Mlo.
DR   PANTHER; PTHR31942; PTHR31942; 1.
DR   Pfam; PF03094; Mlo; 1.
PE   2: Evidence at transcript level;
KW   Calmodulin-binding; Membrane; Pathogenesis-related protein; Plant defense;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..501
FT                   /note="MLO-like protein 5"
FT                   /id="PRO_0000209935"
FT   TOPO_DOM        1..22
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        23..43
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        44..68
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        90..151
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        173..273
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        295
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        296..316
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        317..359
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        360..380
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        381..391
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        413..501
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          443..501
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          450..471
FT                   /note="Calmodulin-binding"
FT   COMPBIAS        474..501
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        458
FT                   /note="Missing (in Ref. 1; AAK53798)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   501 AA;  56896 MW;  4B3F95D0FB18DCF8 CRC64;
     MAGGGGGSTS GEGPRELDQT PTWAVSTVCG VIILISIVLE LMIHKIGEVF TERRKKALYE
     ALQKIKNELM VLGFISLLLT FGQNYIASLC VASRYGHAMS FCGPYDGPSG ESKKPKTTEH
     LERRVLADAA PAQCKKGYVP LISLNALHQV HIFIFFLAVF HVIYSAITMM LGRAKIRGWK
     VWEEEVINDH EMMNDPSRFR LTHETSFVRE HVNPWAKNRF SFYVMCFFRQ MLRSVRKSDY
     LTMRHGFISV HLAPGMKFNF QKYIKRSLED DFKVVVGISP ELWAFVMLFL LFDVHGWYVT
     AVITMIPPLL TLAIGTKLQA IISDMALEIQ ERHAVIQGMP LVNVSDRHFW FSRPALVLHI
     IHFILFQNAF EITYFFWIWY EFGLRSCFHH HFALIIIRVA LGVGVQFLCS YITLPLYALV
     TQMGSTMKRS VFDDQTSKAL KNWHKNAKKK SETPGQTQPP LPNLRPKTGG DIESASPANI
     TASVDVKESD QSQSRDLLSG P