MLO_HORVU
ID MLO_HORVU Reviewed; 533 AA.
AC P93766;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Protein MLO;
GN Name=MLO;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTANTS MLO-1; MLO-7; MLO-9; MLO-10;
RP MLO-13; MLO-17 AND MLO-26.
RC STRAIN=cv. Carlsberg II, cv. Diamant, cv. Foma, cv. Haisa, cv. Ingrid,
RC cv. Malteria Heda, and cv. Plena; TISSUE=Leaf;
RX PubMed=9054509; DOI=10.1016/s0092-8674(00)81912-1;
RA Bueschges R., Hollricher K., Panstruga R., Simons G., Wolter M.,
RA Frijters A., van Daelen R., van der Lee T., Diergaarde P., Groenendijk J.,
RA Toepsch S., Vos P., Salamini F., Schulze-Lefert P.;
RT "The barley Mlo gene: a novel control element of plant pathogen
RT resistance.";
RL Cell 88:695-705(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Ingrid;
RX PubMed=9461468; DOI=10.1093/nar/26.4.1056;
RA Panstruga R., Buschges R., Piffanelli P., Schulze-Lefert P.;
RT "A contiguous 60 kb genomic stretch from barley reveals molecular evidence
RT for gene islands in a monocot genome.";
RL Nucleic Acids Res. 26:1056-1062(1998).
RN [3]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Ingrid;
RX PubMed=10574976; DOI=10.1074/jbc.274.49.34993;
RA Devoto A., Piffanelli P., Nilsson I., Wallin E., Panstruga R.,
RA von Heijne G., Schulze-Lefert P.;
RT "Topology, subcellular localization, and sequence diversity of the Mlo
RT family in plants.";
RL J. Biol. Chem. 274:34993-35004(1999).
RN [4]
RP INTERACTION WITH CALMODULIN, AND MUTAGENESIS OF LEU-420 AND TRP-423.
RX PubMed=11919636; DOI=10.1038/416447a;
RA Kim M.C., Panstruga R., Elliott C., Mueller J., Devoto A., Yoon H.W.,
RA Park H.C., Cho M.J., Schulze-Lefert P.;
RT "Calmodulin interacts with MLO protein to regulate defence against mildew
RT in barley.";
RL Nature 416:447-451(2002).
RN [5]
RP FUNCTION, INDUCTION, AND MUTANTS MLO-12; MLO-27; MLO-28; MLO-29 AND MLO-30.
RX PubMed=12114562; DOI=10.1104/pp.010954;
RA Piffanelli P., Zhou F., Casais C., Orme J., Jarosch B., Schaffrath U.,
RA Collins N.C., Panstruga R., Schulze-Lefert P.;
RT "The barley MLO modulator of defense and cell death is responsive to biotic
RT and abiotic stress stimuli.";
RL Plant Physiol. 129:1076-1085(2002).
CC -!- FUNCTION: Seems to be involved in modulation of pathogen defense and
CC leaf cell death. Down-regulates broad spectrum resistance to powdery
CC mildew fungus and its defense suppression activity is enhanced by
CC Ca(2+)-dependent calmodulin binding. Signaling seems not to require
CC heterotrimeric G proteins. {ECO:0000269|PubMed:12114562}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10574976}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:10574976}.
CC -!- INDUCTION: By fungal infection and abiotic stress.
CC {ECO:0000269|PubMed:12114562}.
CC -!- DOMAIN: The C-terminus contains a calmodulin-binding domain, which
CC binds calmodulin in a calcium-dependent fashion.
CC -!- MISCELLANEOUS: Mutations in the MLO gene confer broad spectrum
CC resistance to the powdery mildew fungus and a enhanced susceptibility
CC to fungal pathogens Magnaporthe grisea and Bipolaris sorokiniana.
CC -!- SIMILARITY: Belongs to the MLO family. {ECO:0000305}.
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DR EMBL; Z83834; CAB06083.1; -; mRNA.
DR EMBL; Y14573; CAA74909.1; -; Genomic_DNA.
DR PIR; T04481; T04481.
DR AlphaFoldDB; P93766; -.
DR SMR; P93766; -.
DR TCDB; 9.B.197.1.1; the mildew resistance locus o (mlo) family.
DR EnsemblPlants; HORVU.MOREX.r2.4HG0342080.1; HORVU.MOREX.r2.4HG0342080.1; HORVU.MOREX.r2.4HG0342080.
DR EnsemblPlants; HORVU.MOREX.r2.4HG0342080.1.mrna1; HORVU.MOREX.r2.4HG0342080.1.mrna1; HORVU.MOREX.r2.4HG0342080.1.
DR Gramene; HORVU.MOREX.r2.4HG0342080.1; HORVU.MOREX.r2.4HG0342080.1; HORVU.MOREX.r2.4HG0342080.
DR Gramene; HORVU.MOREX.r2.4HG0342080.1.mrna1; HORVU.MOREX.r2.4HG0342080.1.mrna1; HORVU.MOREX.r2.4HG0342080.1.
DR OMA; LWYAGDA; -.
DR ExpressionAtlas; P93766; differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR InterPro; IPR004326; Mlo.
DR PANTHER; PTHR31942; PTHR31942; 1.
DR Pfam; PF03094; Mlo; 1.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Membrane; Pathogenesis-related protein; Plant defense;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..533
FT /note="Protein MLO"
FT /id="PRO_0000209928"
FT TOPO_DOM 1..17
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..251
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..335
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 357..379
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..533
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 414..435
FT /note="Calmodulin-binding"
FT REGION 446..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 511..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 10
FT /note="R->W: In mlo-9; confers broad spectrum resistance to
FT powdery mildew fungus."
FT MUTAGEN 30
FT /note="V->E: In mlo-13; confers broad spectrum resistance
FT to powdery mildew fungus."
FT MUTAGEN 31
FT /note="S->F: In mlo-17; confers broad spectrum resistance
FT to powdery mildew fungus."
FT MUTAGEN 162
FT /note="W->R: In mlo-1; confers broad spectrum resistance to
FT powdery mildew fungus."
FT MUTAGEN 182..183
FT /note="Missing: In mlo-10; confers broad spectrum
FT resistance to powdery mildew fungus."
FT MUTAGEN 222
FT /note="T->I: In mlo-28; confers partial resistance to
FT powdery mildew fungus."
FT MUTAGEN 226
FT /note="G->D: In mlo-7; confers broad spectrum resistance to
FT powdery mildew fungus."
FT MUTAGEN 240
FT /note="F->L: In mlo-12; confers partial resistance to
FT powdery mildew fungus."
FT MUTAGEN 270
FT /note="L->H: In mlo-26; confers broad spectrum resistance
FT to powdery mildew fungus."
FT MUTAGEN 318
FT /note="G->E: In mlo-27; confers broad spectrum resistance
FT to powdery mildew fungus."
FT MUTAGEN 334
FT /note="P->L: In mlo-29; confers broad spectrum resistance
FT to powdery mildew fungus."
FT MUTAGEN 403..408
FT /note="Missing: In mlo-30; confers broad spectrum
FT resistance to powdery mildew fungus."
FT MUTAGEN 420
FT /note="L->R: Diminishes binding to calmodulin and reduces
FT susceptibility to powdery mildew fungus."
FT /evidence="ECO:0000269|PubMed:11919636"
FT MUTAGEN 423
FT /note="W->R: Diminishes binding to calmodulin and reduces
FT susceptibility to powdery mildew fungus."
FT /evidence="ECO:0000269|PubMed:11919636"
SQ SEQUENCE 533 AA; 60413 MW; AC0D832EE14F7D15 CRC64;
MSDKKGVPAR ELPETPSWAV AVVFAAMVLV SVLMEHGLHK LGHWFQHRHK KALWEALEKM
KAELMLVGFI SLLLIVTQDP IIAKICISED AADVMWPCKR GTEGRKPSKY VDYCPEGKVA
LMSTGSLHQL HVFIFVLAVF HVTYSVITIA LSRLKMRTWK KWETETTSLE YQFANDPARF
RFTHQTSFVK RHLGLSSTPG IRWVVAFFRQ FFRSVTKVDY LTLRAGFINA HLSQNSKFDF
HKYIKRSMED DFKVVVGISL PLWGVAILTL FLDINGVGTL IWISFIPLVI LLCVGTKLEM
IIMEMALEIQ DRASVIKGAP VVEPSNKFFW FHRPDWVLFF IHLTLFQNAF QMAHFVWTVA
TPGLKKCYHT QIGLSIMKVV VGLALQFLCS YMTFPLYALV TQMGSNMKRS IFDEQTSKAL
TNWRNTAKEK KKVRDTDMLM AQMIGDATPS RGSSPMPSRG SSPVHLLHKG MGRSDDPQSA
PTSPRTQQEA RDMYPVVVAH PVHRLNPNDR RRSASSSALE ADIPSADFSF SQG
