MLPA_BORBU
ID MLPA_BORBU Reviewed; 148 AA.
AC H7C7P1;
DT 13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2012, sequence version 1.
DT 25-MAY-2022, entry version 44.
DE RecName: Full=Lipoprotein MlpA {ECO:0000303|PubMed:10948116};
DE Flags: Precursor;
GN Name=mlpA; OrderedLocusNames=BB_P28;
OS Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS (Borrelia burgdorferi).
OG Plasmid cp32-1.
OC Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX NCBI_TaxID=224326;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=9403685; DOI=10.1038/37551;
RA Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA Smith H.O., Venter J.C.;
RT "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL Nature 390:580-586(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=10672174; DOI=10.1046/j.1365-2958.2000.01698.x;
RA Casjens S., Palmer N., van Vugt R., Huang W.M., Stevenson B., Rosa P.,
RA Lathigra R., Sutton G.G., Peterson J.D., Dodson R.J., Haft D.H.,
RA Hickey E.K., Gwinn M.L., White O., Fraser C.M.;
RT "A bacterial genome in flux: the twelve linear and nine circular
RT extrachromosomal DNAs in an infectious isolate of the Lyme disease
RT spirochete Borrelia burgdorferi.";
RL Mol. Microbiol. 35:490-516(2000).
RN [3]
RP FUNCTION, ANTIGENICITY, SUBCELLULAR LOCATION, AND INDUCTION AT 35 DEGREES
RP CELSIUS.
RC STRAIN=B31-4A; PLASMID=cp32-1;
RX PubMed=10948116; DOI=10.1128/iai.68.9.4992-5001.2000;
RA Porcella S.F., Fitzpatrick C.A., Bono J.L.;
RT "Expression and immunological analysis of the plasmid-borne mlp genes of
RT Borrelia burgdorferi strain B31.";
RL Infect. Immun. 68:4992-5001(2000).
CC -!- FUNCTION: An outer membrane protein that may participate in
CC pathogenesis. Some human Lyme disease patients have antibodies against
CC this protein (PubMed:10948116). The Mlp proteins probably undergo
CC intragenic recombination, generating new alleles (Probable).
CC {ECO:0000269|PubMed:10948116, ECO:0000305|PubMed:10948116}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:10948116}; Lipid-anchor
CC {ECO:0000305|PubMed:10948116}.
CC -!- INDUCTION: Strongly induced when grown at 35 degrees Celsius.
CC {ECO:0000269|PubMed:10948116}.
CC -!- SIMILARITY: Belongs to the Multicopy lipoprotein (Mlp) family.
CC {ECO:0000305}.
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DR EMBL; AE001575; AAF07417.1; -; Genomic_DNA.
DR RefSeq; NP_051189.1; NC_000948.1.
DR RefSeq; WP_010883731.1; NC_000948.1.
DR PDB; 6QBI; NMR; -; A=51-148.
DR PDBsum; 6QBI; -.
DR AlphaFoldDB; H7C7P1; -.
DR BMRB; H7C7P1; -.
DR SMR; H7C7P1; -.
DR EnsemblBacteria; AAF07417; AAF07417; BB_P28.
DR KEGG; bbu:BB_P28; -.
DR PATRIC; fig|224326.49.peg.28; -.
DR HOGENOM; CLU_134260_0_0_12; -.
DR Proteomes; UP000001807; Plasmid cp32-1.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR004983; Mlp.
DR Pfam; PF03304; Mlp; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW Plasmid; Reference proteome; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000305"
FT CHAIN 18..148
FT /note="Lipoprotein MlpA"
FT /evidence="ECO:0000305"
FT /id="PRO_5003609303"
FT REGION 26..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..58
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 18
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 18
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
FT HELIX 54..61
FT /evidence="ECO:0007829|PDB:6QBI"
FT HELIX 64..77
FT /evidence="ECO:0007829|PDB:6QBI"
FT HELIX 80..87
FT /evidence="ECO:0007829|PDB:6QBI"
FT HELIX 91..106
FT /evidence="ECO:0007829|PDB:6QBI"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:6QBI"
FT HELIX 113..125
FT /evidence="ECO:0007829|PDB:6QBI"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:6QBI"
FT TURN 137..139
FT /evidence="ECO:0007829|PDB:6QBI"
FT TURN 143..145
FT /evidence="ECO:0007829|PDB:6QBI"
SQ SEQUENCE 148 AA; 16413 MW; 97C9ACAA3026EAFF CRC64;
MKIINILFCL FLLLLNSCNS NDNDTLKNNA QQTKSRGKRD LTQKEATPEK PKSKEELLRE
KLSEDQKTHL DWLKEALGND GEFDKFLGYD ESKIKTALDH IKSELDKCNG NDADQQKTTF
KQTVQGALSG GIDGFGSNNA VTTCGNGS